Posts in Core Rigor and Reproducibility (CoRRe)

Dear colleagues,

Thanks to many of you who have completed the new survey from the ABRF Committee
on Core Rigor and Reproducibility (CCoRRe).  Our goal is to assess how (or if)
awareness and implementation of guidelines, policies, and best practices for
rigor, reproducibility and transparency have shifted in the ensuing four years
since our 2017 survey on this topic (learn more: Knudtson et al, J Biomol Tech.
2019 Sep;30(3):36-44).

We are still collecting responses, and welcome participation from you and
anyone who uses or is associated with core facilities – feel free to share the
survey link to anyone in your network.  You can access our new survey here:
https://redcap.link/ABRF-Cores-and-RRT. Responses are anonymous, and it should
take less than 10 minutes to complete.

Thank you!
ABRF Committee on Core Rigor and Reproducibility (CCoRRe)
https://www.abrf.org/core-rigor-and-reproducibility-ccorre-

Susan Meyn, Vanderbilt University Medical Center (Chair)
Rebecca Davies, University of Minnesota
Kevin Knudtson, University of Iowa (Executive Board Liaison)
Christopher Gregory, University of North Carolina School of Medicine
Adrian Halme, University of Virginia
Sheenah Mische, NYU Langone Medical Center
Andrew Ott, Northwestern University
Katia Sol-Church, University of Virginia
Michael Sturges, Miltenyi Biotech

Dear colleagues,

Thanks to many of you who have completed the new survey from the ABRF Committee
on Core Rigor and Reproducibility (CCoRRe).  Our goal is to assess how (or if)
awareness and implementation of guidelines, policies, and best practices for
rigor, reproducibility and transparency have shifted in the ensuing four years
since our 2017 survey on this topic (learn more: Knudtson et al, J Biomol Tech.
2019 Sep;30(3):36-44).

We are still collecting responses, and welcome participation from you and
anyone who uses or is associated with core facilities – feel free to share the
survey link to anyone in your network.  You can access our new survey here:
https://redcap.link/ABRF-Cores-and-RRT. Responses are anonymous, and it should
take less than 10 minutes to complete.

Thank you!
ABRF Committee on Core Rigor and Reproducibility (CCoRRe)
https://www.abrf.org/core-rigor-and-reproductibility-ccore-

Susan Meyn, Vanderbilt University Medical Center (Chair)
Rebecca Davies, University of Minnesota
Kevin Knudtson, University of Iowa (Executive Board Liaison)
Christopher Gregory, University of North Carolina School of Medicine
Adrian Halme, University of Virginia
Sheenah Mische, NYU Langone Medical Center
Andrew Ott, Northwestern University
Katia Sol-Church, University of Virginia
Michael Sturges, Miltenyi Biotech

The 2022 ABRF Annual Meeting Program Committee would like your help to design
the program for the upcoming.

Please take a moment to complete this brief survey on the potential topics and
format for the meeting:

https://abrf.memberclicks.net/2022-abrf-annual-meeting-survey


Responses received by Friday, April 29 will be eligible for a drawing to
receive complimentary registration for the meeting.

Thank you.

How to Leverage CoreMarketplace and RRIDs to Raise Awareness
of Your Core Facility


The ABRF Committee on Core Rigor and Reproducibility (CCoRRe) invites you to
join the April ABRF Town Hall to learn more about how core facilities can
capitalize on tools to increase recognition for their role in advancing
research.  Participants will learn more about the role of CoreMarketplace and
to effectively use it, how Research Resource Identifiers (RRIDs) fit in the
research community, and how one leading institution is implementing these tools
to realize their vision for research cores.

Speakers:
Nate Herzog, CoreMarketplace Project Lead, Vermont Genetics Network
Anita Bandrowski, CEO, SciCrunch
Claudius Mundoma, University of Colorado Boulder


Register here:
http://abrf.memberclicks.net/message2/link/a7b1f5e4-4d6a-4c80-b43a-c54f723695ff/1

Following the presentation, there will be an Open Forum for participants to
discuss other topics or ask questions about ABRF activities.

Contact ABRF if you have any questions.

Hello Susan,
I am interested, but not currently and ABRF member. Would like to join and
ready to do so. Assume I can join as an individual member and then apply to
participate in CCoRRe with my CV and letter of intent. Please confirm that this
is the path I should follow.

Regards,
Michael

-----Original Message-----
From: Core Rigor and Reproducibility (CoRRe) <email obscured>> On Behalf Of
Meyn, Susan M
Sent: Tuesday, February 23, 2021 9:31 AM
To: Core Rigor and Reproducibility (CoRRe) <email obscured>>
Subject: [EXTERNAL]Re: [core rigor and reproducibility (corre)] Call for new
members: ABRF Committee for Core Rigor and Reproducibility (CCoRRe)

WARNING: This email was sent from an address outside the company. Do not click
on any links or open any file attachments unless you recognize the sender and
are confident the content is safe.

Hi Michael,

Thanks for your interest, we definitely welcome supplier participation as
members of  CCoRRe!  To be eligible to serve, individuals should be ABRF
members in good standing throughout their time of service on the committee.
More generally, all are welcome to participate in open discussion on topics of
shared interest via this listserv.

Let me know if you have any other questions - happy to set up time for a chat.

Thanks again,
Susan

Susan Meyn
Office of Research | Vanderbilt University Medical Center
Twitter: @VUMCResearch | <email obscured><email obscured>> | 615.322.0470


From: Michael Sturges
<email obscured><email obscured>>>
Date: Mon, Feb 22, 2021 at 4:48 PM
Subject: Re: [core rigor and reproducibility (corre)] [EXTERNAL] Call for new
members: ABRF Committee for Core Rigor and Reproducibility (CCoRRe)
To: <email obscured><email obscured>>
<email obscured><email obscured>>>


Hello ABRF CORRE Team,
Are individuals from the life science tools supplier side welcome to
participate as either team members or extended team members?

Regards,
Michael


Michael R. Sturges, Ph.D.
Marketing Manager
Miltenyi Biotec, Inc.

Office 858 202 0705
Mobile 619 481 2223
Email <email obscured><email obscured>>
Technical Support Phone 866 426 1194
Technical Support Email <email obscured><email obscured>>

https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.miltenyibiotec.com%2F&amp;data=04%7C01%7C%7C7d5b316530c24f9aa6c908d8d820c1ee%7Cf54d3069b76d43d9b2f14a23ea2ca7ce%7C0%7C0%7C637496982499931451%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=1hOvCxINp%2F2bKZVe8G%2Fb9nupbA7WPcfhGI%2FG6RZWnOg%3D&amp;reserved=0<https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.miltenyibiotec.com%2F&amp;data=04%7C01%7C%7C7d5b316530c24f9aa6c908d8d820c1ee%7Cf54d3069b76d43d9b2f14a23ea2ca7ce%7C0%7C0%7C637496982499931451%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=1hOvCxINp%2F2bKZVe8G%2Fb9nupbA7WPcfhGI%2FG6RZWnOg%3D&amp;reserved=0>

Miltenyi Biotec Flow Cytometry Instruments and Reagents -Join the Flow
Revolution!





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Unless you are the addressee (or authorized to receive for the addressee), you
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-----Original Message-----
From: Core Rigor and Reproducibility (CoRRe)
<email obscured><email obscured>>> On Behalf Of Meyn, Susan M
Sent: Monday, February 22, 2021 7:42 AM
To: Core Rigor and Reproducibility (CoRRe)
<email obscured><email obscured>>>
Subject: [EXTERNAL][core rigor and reproducibility (corre)] Call for new
members: ABRF Committee for Core Rigor and Reproducibility (CCoRRe)

WARNING: This email was sent from an address outside the company. Do not click
on any links or open any file attachments unless you recognize the sender and
are confident the content is safe.

The Committee for Core Rigor and Reproducibility (CCoRRe) is seeking to add new
members to join in our mission to promote resources for the ABRF membership in
achieving accurate and reproducible results in their shared resource
facilities.  This is a great opportunity for an interested ABRF members to work
with a dynamic team and make valuable contributions to the advancement of
better science.  You can learn more about CCoRRe below, or by visiting our
webpage:
https://eur02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.abrf.org%2Fcore-rigor-and-reproductibility-ccore-&amp;data=04%7C01%7C%7C7d5b316530c24f9aa6c908d8d820c1ee%7Cf54d3069b76d43d9b2f14a23ea2ca7ce%7C0%7C0%7C637496982499941445%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=X7ZYrTZbfosn6pnXdCmhE0oHBvvxILcYCOfDft5kou8%3D&amp;reserved=0<https://eur02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.abrf.org%2Fcore-rigor-and-reproductibility-ccore-&amp;data=04%7C01%7C%7C7d5b316530c24f9aa6c908d8d820c1ee%7Cf54d3069b76d43d9b2f14a23ea2ca7ce%7C0%7C0%7C637496982499941445%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=X7ZYrTZbfosn6pnXdCmhE0oHBvvxILcYCOfDft5kou8%3D&amp;reserved=0>
If you are an ABRF member in good standing and interested in joining CCoRRe,
please send CV and a letter of intent that outlines your interest and ideas
related to the role of cores and/or ABRF in advancing rigorous and reproducible
science, to Susan Meyn at
<email obscured><email obscured><email obscured><email obscured>>>.
Thank you!
The Committee for Core Rigor and Reproducibility (CCoRRe):
Katia Sol Church, University of Virginia School of Medicine Rebecca Davies,
University of Minnesota Chris Gregory, University of North Carolina School of
Medicine Adrian Halme, University of Virginia Kevin Knudtson, University of
Iowa (EB liaison) Susan Meyn, Vanderbilt University Medical Center (Chair)
Sheenah Mische, NYU Langone Medical Center Frances Weis-Garcia, Memorial Sloan
Kettering Cancer Center



About CCoRRE:
The mission of the ABRF Committee on Core Rigor and Reproducibility (CCoRRe) is
to enable rigorous and reproducible research through support of shared
scientific resources in advancing technology, education and communication. Core
facilities support a significant portion of the research conducted at
biomolecular research institutions, thus playing a critical role in achieving
efficient use of research funds and broadening access to advanced skills,
expertise and technologies. Shared scientific research resources generate the
majority of research data at many institutions, so their role in maintaining
necessary expertise and generating high quality data is considerable. CCoRRe
efforts are focused on building educational resources, sharing critical best
practice guidelines, and providing important tools to the core community that
will impact rigor, reproducibility and transparency across the range of science
and technology.
New members of CCoRRe will contribute to broadening our scope beyond NIH-driven
considerations, and developing resources that will support the ABRF community
in pursuing and advocating for rigor, reproducibility and transparency in all
areas of scientific endeavor.  CCoRRe plans for 2021 include:

  *   Develop an improved website (currently
https://eur02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fabrf.org%2Fcommittee%2Fcommittee-core-rigor-and-reproducibility-ccorre&amp;data=04%7C01%7C%7C7d5b316530c24f9aa6c908d8d820c1ee%7Cf54d3069b76d43d9b2f14a23ea2ca7ce%7C0%7C0%7C637496982499941445%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=dzvp3lYMtUIGkw3BxdDQFjdRKyeiVktAXQ08mkUH148%3D&amp;reserved=0<https://eur02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fabrf.org%2Fcommittee%2Fcommittee-core-rigor-and-reproducibility-ccorre&amp;data=04%7C01%7C%7C7d5b316530c24f9aa6c908d8d820c1ee%7Cf54d3069b76d43d9b2f14a23ea2ca7ce%7C0%7C0%7C637496982499941445%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=dzvp3lYMtUIGkw3BxdDQFjdRKyeiVktAXQ08mkUH148%3D&amp;reserved=0>)
with educational links and best-practice protocols that can be applied across
ABRF members' scientific disciplines.
  *   Identify opportunities for outreach and partnership with other
professional societies and scientific journals, to enable and sustain
partnerships that advance our mission.
  *   Conduct a follow up to the Survey on Scientific Shared Resource Rigor and
Reproducibility conducted by CCoRRe and reported out during 2018.  The aim of
this project is to learn how the knowledge, understanding and implementation of
RR&T principles has changed in the last 3 years.  To ensure best points of
comparison, the original survey questions will not be changed, but we will add
a few new questions that focus on challenges relating to the COVID-19 pandemic.
We plan to analyze and publish a report on our findings during 2021.
  *   Continue to work with ABRF Core MarketPlace (CMP) to expand use of CMP
with integrated Research Resource Identification (RRID), and to advance a
working group approach to guide the overall project activities.


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Hello ABRF CORRE Team,
Are individuals from the life science tools supplier side welcome to
participate as either team members or extended team members?

Regards,
Michael


Michael R. Sturges, Ph.D.
Marketing Manager
Miltenyi Biotec, Inc.

Office 858 202 0705
Mobile 619 481 2223
Email <email obscured>
Technical Support Phone 866 426 1194
Technical Support Email <email obscured>

www.miltenyibiotec.com

Miltenyi Biotec Flow Cytometry Instruments and Reagents -Join the Flow
Revolution!

This message contains information which may be confidential and privileged.
Unless you are the addressee (or authorized to receive for the addressee), you
may not use, copy or disclose to anyone the message or any information
contained in the message. If you have received the message in error, please
advise the sender by reply e-mail, and delete the message.





-----Original Message-----
From: Core Rigor and Reproducibility (CoRRe) <email obscured>> On Behalf Of
Meyn, Susan M
Sent: Monday, February 22, 2021 7:42 AM
To: Core Rigor and Reproducibility (CoRRe) <email obscured>>
Subject: [EXTERNAL][core rigor and reproducibility (corre)] Call for new
members: ABRF Committee for Core Rigor and Reproducibility (CCoRRe)

WARNING: This email was sent from an address outside the company. Do not click
on any links or open any file attachments unless you recognize the sender and
are confident the content is safe.

The Committee for Core Rigor and Reproducibility (CCoRRe) is seeking to add new
members to join in our mission to promote resources for the ABRF membership in
achieving accurate and reproducible results in their shared resource
facilities.  This is a great opportunity for an interested ABRF members to work
with a dynamic team and make valuable contributions to the advancement of
better science.  You can learn more about CCoRRe below, or by visiting our
webpage:
https://eur02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.abrf.org%2Fcore-rigor-and-reproductibility-ccore-&amp;data=04%7C01%7C%7C9fda3e0c2c29498bbfc208d8d7485a46%7Cf54d3069b76d43d9b2f14a23ea2ca7ce%7C0%7C0%7C637496053052668496%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=pTvc3ajDTprVAlTUQA7X0vQJyLqh3iihXUWvtkGixHo%3D&amp;reserved=0
If you are an ABRF member in good standing and interested in joining CCoRRe,
please send CV and a letter of intent that outlines your interest and ideas
related to the role of cores and/or ABRF in advancing rigorous and reproducible
science, to Susan Meyn at <email obscured><email obscured>>.
Thank you!
The Committee for Core Rigor and Reproducibility (CCoRRe):
Katia Sol Church, University of Virginia School of Medicine Rebecca Davies,
University of Minnesota Chris Gregory, University of North Carolina School of
Medicine Adrian Halme, University of Virginia Kevin Knudtson, University of
Iowa (EB liaison) Susan Meyn, Vanderbilt University Medical Center (Chair)
Sheenah Mische, NYU Langone Medical Center Frances Weis-Garcia, Memorial Sloan
Kettering Cancer Center



About CCoRRE:
The mission of the ABRF Committee on Core Rigor and Reproducibility (CCoRRe) is
to enable rigorous and reproducible research through support of shared
scientific resources in advancing technology, education and communication. Core
facilities support a significant portion of the research conducted at
biomolecular research institutions, thus playing a critical role in achieving
efficient use of research funds and broadening access to advanced skills,
expertise and technologies. Shared scientific research resources generate the
majority of research data at many institutions, so their role in maintaining
necessary expertise and generating high quality data is considerable. CCoRRe
efforts are focused on building educational resources, sharing critical best
practice guidelines, and providing important tools to the core community that
will impact rigor, reproducibility and transparency across the range of science
and technology.
New members of CCoRRe will contribute to broadening our scope beyond NIH-driven
considerations, and developing resources that will support the ABRF community
in pursuing and advocating for rigor, reproducibility and transparency in all
areas of scientific endeavor.  CCoRRe plans for 2021 include:

  *   Develop an improved website (currently
https://eur02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fabrf.org%2Fcommittee%2Fcommittee-core-rigor-and-reproducibility-ccorre&amp;data=04%7C01%7C%7C9fda3e0c2c29498bbfc208d8d7485a46%7Cf54d3069b76d43d9b2f14a23ea2ca7ce%7C0%7C0%7C637496053052668496%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=%2BmLUDmfJxHPmaJ6yaEMeVSiCIGuncYU%2FdbzTjgh6oyY%3D&amp;reserved=0)
with educational links and best-practice protocols that can be applied across
ABRF members' scientific disciplines.
  *   Identify opportunities for outreach and partnership with other
professional societies and scientific journals, to enable and sustain
partnerships that advance our mission.
  *   Conduct a follow up to the Survey on Scientific Shared Resource Rigor and
Reproducibility conducted by CCoRRe and reported out during 2018.  The aim of
this project is to learn how the knowledge, understanding and implementation of
RR&T principles has changed in the last 3 years.  To ensure best points of
comparison, the original survey questions will not be changed, but we will add
a few new questions that focus on challenges relating to the COVID-19 pandemic.
We plan to analyze and publish a report on our findings during 2021.
  *   Continue to work with ABRF Core MarketPlace (CMP) to expand use of CMP
with integrated Research Resource Identification (RRID), and to advance a
working group approach to guide the overall project activities.


――
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Leave group <email obscured>?Subject=Unsubscribe

The Committee for Core Rigor and Reproducibility (CCoRRe) is seeking to add new
members to join in our mission to promote resources for the ABRF membership in
achieving accurate and reproducible results in their shared resource
facilities.  This is a great opportunity for an interested ABRF members to work
with a dynamic team and make valuable contributions to the advancement of
better science.  You can learn more about CCoRRe below, or by visiting our
webpage: https://www.abrf.org/core-rigor-and-reproductibility-ccore-
If you are an ABRF member in good standing and interested in joining CCoRRe,
please send CV and a letter of intent that outlines your interest and ideas
related to the role of cores and/or ABRF in advancing rigorous and reproducible
science, to Susan Meyn at <email obscured><email obscured>>.
Thank you!
The Committee for Core Rigor and Reproducibility (CCoRRe):
Katia Sol Church, University of Virginia School of Medicine
Rebecca Davies, University of Minnesota
Chris Gregory, University of North Carolina School of Medicine
Adrian Halme, University of Virginia
Kevin Knudtson, University of Iowa (EB liaison)
Susan Meyn, Vanderbilt University Medical Center (Chair)
Sheenah Mische, NYU Langone Medical Center
Frances Weis-Garcia, Memorial Sloan Kettering Cancer Center



About CCoRRE:
The mission of the ABRF Committee on Core Rigor and Reproducibility (CCoRRe) is
to enable rigorous and reproducible research through support of shared
scientific resources in advancing technology, education and communication. Core
facilities support a significant portion of the research conducted at
biomolecular research institutions, thus playing a critical role in achieving
efficient use of research funds and broadening access to advanced skills,
expertise and technologies. Shared scientific research resources generate the
majority of research data at many institutions, so their role in maintaining
necessary expertise and generating high quality data is considerable. CCoRRe
efforts are focused on building educational resources, sharing critical best
practice guidelines, and providing important tools to the core community that
will impact rigor, reproducibility and transparency across the range of science
and technology.
New members of CCoRRe will contribute to broadening our scope beyond NIH-driven
considerations, and developing resources that will support the ABRF community
in pursuing and advocating for rigor, reproducibility and transparency in all
areas of scientific endeavor.  CCoRRe plans for 2021 include:

  *   Develop an improved website (currently
https://abrf.org/committee/committee-core-rigor-and-reproducibility-ccorre)
with educational links and best-practice protocols that can be applied across
ABRF members’ scientific disciplines.
  *   Identify opportunities for outreach and partnership with other
professional societies and scientific journals, to enable and sustain
partnerships that advance our mission.
  *   Conduct a follow up to the Survey on Scientific Shared Resource Rigor and
Reproducibility conducted by CCoRRe and reported out during 2018.  The aim of
this project is to learn how the knowledge, understanding and implementation of
RR&T principles has changed in the last 3 years.  To ensure best points of
comparison, the original survey questions will not be changed, but we will add
a few new questions that focus on challenges relating to the COVID-19 pandemic.
We plan to analyze and publish a report on our findings during 2021.
  *   Continue to work with ABRF Core MarketPlace (CMP) to expand use of CMP
with integrated Research Resource Identification (RRID), and to advance a
working group approach to guide the overall project activities.

Found quite a few have been given for a few at MSKCC … for example.



  *
SCR_017850<https://scicrunch.org/resources/Any/search?q=SCR_017850&l=SCR_017850>
- Molecular Cytogenetics Core Facility
  *
SCR_017691<https://scicrunch.org/resources/Any/search?q=SCR_017691&l=SCR_017691>
- Antibody & Bioresource Core Facility
  *
SCR_017853<https://scicrunch.org/resources/Any/search?q=SCR_017853&l=SCR_017853>
- Investigational Products Support Core Facility

From: Frances Weis-Garcia <email obscured>>
Date: Tuesday, June 2, 2020 at 11:48 AM
To: "Core Rigor and Reproducibility (CoRRe)" <email obscured>>
Subject: We have an RRID number

We have an RRID number
But cannot find other cores with RRID numbers (yet)

Only matters if we want to define the structure of the core RRID

[A screenshot of a cell phone  Description automatically generated]

image001.png 377.3kb

Sure!!

Laura Bover, PhD
Professor
Director Monoclonal Antibody Core Facility
University of Texas M.D.Anderson Cancer Center
Associate member of The University of Texas Graduate School of Biomedical
Sciences at Houston
Member of the Scientific Review Committee 2 (former CRC 2)
Member of the Steering Committee of the Immunology Program GSBS
Immunology Department/ Genomics Medicine Department
7455 Fannin St<x-apple-data-detectors://1/1> Room 1SCR4.2021
Houston- TX 77054<x-apple-data-detectors://2/0>
T  713-563-3301<tel:713-563-3301>
F 713-563-3276<tel:713-563-3276>
<email obscured><email obscured>>

On Jan 22, 2020, at 12:04 PM, Frances Weis-Garcia
<email obscured>> wrote:

I'm so glad you are attending.
We have to make time for a drink.

Frances

ZRC 1563
Office     646-888-2354
Mobile   516-967-0656


On 1/22/20, 1:01 PM, "Core Rigor and Reproducibility (CoRRe) on behalf of
Bover,Laura" <email obscured> on behalf of <email obscured>> wrote:

   Thanks for the clarification! Obviously I was thinking in my type of
experiments, trying to identify new markers upon treatment with certain agents.
   We used to have a Core here for identification of cell lines, but I am not
sure of it is still operating.
   Thanks!
   And see you soon at ABRF meeting!
   Laura

   Laura Bover, PhD
   Professor
   Director Monoclonal Antibody Core Facility
   University of Texas M.D.Anderson Cancer Center
   Associate member of The University of Texas Graduate School of Biomedical
Sciences at Houston
   Member of the Scientific Review Committee 2 (former Clinical Research
Committee 2)
   Member of the Steering Committee of the Immunology Program GSBS
   Immunology Department/ Genomics Medicine Department
   7455 Fannin St Room 1SCR4.2021
   Houston- TX 77054
   T  713-563-3301 <tel:713-563-3301>
   F 713-563-3276 <tel:713-563-3276>
   <email obscured>


   On 1/21/20, 11:22 PM, "Core Rigor and Reproducibility (CoRRe) on behalf of
Frances Weis-Garcia" <email obscured> on behalf of
<email obscured>> wrote:

       Thanks Laura and sorry for the confusion.

       What I am looking for is a way to tell cell line apart if they come from
the same parent.  For human lines that are from different people, STR profiles
are perfect ... but for lines derived from the same original (parent) line ...
there is currently no easy way to tell the difference between parent line A and
children lines A1, A2 much less grandchild A1.3.  Make sense ... If it was a
hybridoma, I would just sequence the CDRs.

       A colleague of mine and I were thinking of inserting a "DNA barcode" in
the lines, probably via CRISPR so we can put in as innocuous a site as
possible, but before developing a solving I was really hoping one already
existed ... so I thought I would poll this group ...

       All about cell line authentication ... one of the many building blocks
needed for RnR.

       Frances

       ZRC 1563
       Office     646-888-2354
       Mobile   516-967-0656


       On 1/21/20, 7:39 PM, "Core Rigor and Reproducibility (CoRRe) on behalf
of Bover,Laura" <email obscured> on behalf of <email obscured>> wrote:

           So, do you need to differentiate the parent cells from the ones
resulted from the division or proliferating?

           May be is not your question.
           What about staining with CFSE a bunch of cells and let them to
divide and sort the different peaks (usually 7, at least in normal cells such
as T cells proliferating) by FACS. The mean fluorescence as you know is going
lower with each division. Is that what you need?
           Depending on the proliferation time you will be able to separate the
last progeny, as well as the intermediate.

           In normal T cells after 5 days you can recover cells from each
division. Not sure if with a cell line you could do the same.

           Laura Bover, PhD
           Professor
           Director Monoclonal Antibody Core Facility
           University of Texas M.D.Anderson Cancer Center
           Associate member of The University of Texas Graduate School of
Biomedical Sciences at Houston
           Member of the Scientific Review Committee 2 (former CRC 2)
           Member of the Steering Committee of the Immunology Program GSBS
           Immunology Department/ Genomics Medicine Department
           7455 Fannin St Room 1SCR4.2021
           Houston- TX 77054
           T  713-563-3301 <tel:713-563-3301>
           F 713-563-3276 <tel:713-563-3276>
           <email obscured>

           In order to continue obtaining Core support in a challenging
environment, it is crucial that our users acknowledge the following grants in
all publications (see following text).  Thank you!
            “This work was supported in part by CCSG grant number P30
CA016672.”

           iLab-Solutions links:
https://mdanderson.ilabsolutions.com/service_center/show_external/3631
           or:  https://mdanderson.ilabsolutions.com/account/login
           If you do not already have an iLab account please create one prior
to sample submission


           On 1/21/20, 8:15 AM, "Core Rigor and Reproducibility (CoRRe) on
behalf of Frances Weis-Garcia" <email obscured> on behalf of
<email obscured>> wrote:

               WARNING: This email originated from outside of MD Anderson.
Please validate the sender's email address before clicking on links or
attachments as they may not be safe.

               Question ... Does anyone know of a way to reliably differentiate
between decedents / children of a cell line as well as the parent?

               One solution is to insert a plasmid to serve as an internal
barcode, but maybe there are other options ...
               ――
               View topic http://list.abrf.org/r/topic/34QaaQAFJeqW0i9xRxpxdF
               Leave group <email obscured>?Subject=Unsubscribe



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Thanks for the clarification! Obviously I was thinking in my type of
experiments, trying to identify new markers upon treatment with certain agents.
We used to have a Core here for identification of cell lines, but I am not sure
of it is still operating.
Thanks!
And see you soon at ABRF meeting!
Laura

Laura Bover, PhD
Professor
Director Monoclonal Antibody Core Facility
University of Texas M.D.Anderson Cancer Center
Associate member of The University of Texas Graduate School of Biomedical
Sciences at Houston
Member of the Scientific Review Committee 2 (former Clinical Research Committee
2)
Member of the Steering Committee of the Immunology Program GSBS
Immunology Department/ Genomics Medicine Department
7455 Fannin St Room 1SCR4.2021
Houston- TX 77054
T  713-563-3301 <tel:713-563-3301>
F 713-563-3276 <tel:713-563-3276>
<email obscured>


On 1/21/20, 11:22 PM, "Core Rigor and Reproducibility (CoRRe) on behalf of
Frances Weis-Garcia" <email obscured> on behalf of
<email obscured>> wrote:

    Thanks Laura and sorry for the confusion.

    What I am looking for is a way to tell cell line apart if they come from
the same parent.  For human lines that are from different people, STR profiles
are perfect ... but for lines derived from the same original (parent) line ...
there is currently no easy way to tell the difference between parent line A and
children lines A1, A2 much less grandchild A1.3.  Make sense ... If it was a
hybridoma, I would just sequence the CDRs.

    A colleague of mine and I were thinking of inserting a "DNA barcode" in the
lines, probably via CRISPR so we can put in as innocuous a site as possible,
but before developing a solving I was really hoping one already existed ... so
I thought I would poll this group ...

    All about cell line authentication ... one of the many building blocks
needed for RnR.

    Frances

    ZRC 1563
    Office     646-888-2354
    Mobile   516-967-0656


    On 1/21/20, 7:39 PM, "Core Rigor and Reproducibility (CoRRe) on behalf of
Bover,Laura" <email obscured> on behalf of <email obscured>> wrote:

        So, do you need to differentiate the parent cells from the ones
resulted from the division or proliferating?

        May be is not your question.
        What about staining with CFSE a bunch of cells and let them to divide
and sort the different peaks (usually 7, at least in normal cells such as T
cells proliferating) by FACS. The mean fluorescence as you know is going lower
with each division. Is that what you need?
        Depending on the proliferation time you will be able to separate the
last progeny, as well as the intermediate.

        In normal T cells after 5 days you can recover cells from each
division. Not sure if with a cell line you could do the same.

        Laura Bover, PhD
        Professor
        Director Monoclonal Antibody Core Facility
        University of Texas M.D.Anderson Cancer Center
        Associate member of The University of Texas Graduate School of
Biomedical Sciences at Houston
        Member of the Scientific Review Committee 2 (former CRC 2)
        Member of the Steering Committee of the Immunology Program GSBS
        Immunology Department/ Genomics Medicine Department
        7455 Fannin St Room 1SCR4.2021
        Houston- TX 77054
        T  713-563-3301 <tel:713-563-3301>
        F 713-563-3276 <tel:713-563-3276>
        <email obscured>

        In order to continue obtaining Core support in a challenging
environment, it is crucial that our users acknowledge the following grants in
all publications (see following text).  Thank you!
         “This work was supported in part by CCSG grant number P30 CA016672.”

        iLab-Solutions links:
        https://mdanderson.ilabsolutions.com/service_center/show_external/3631
        or:  https://mdanderson.ilabsolutions.com/account/login
        If you do not already have an iLab account please create one prior to
sample submission


        On 1/21/20, 8:15 AM, "Core Rigor and Reproducibility (CoRRe) on behalf
of Frances Weis-Garcia" <email obscured> on behalf of
<email obscured>> wrote:

            WARNING: This email originated from outside of MD Anderson. Please
validate the sender's email address before clicking on links or attachments as
they may not be safe.

            Question ... Does anyone know of a way to reliably differentiate
between decedents / children of a cell line as well as the parent?

            One solution is to insert a plasmid to serve as an internal
barcode, but maybe there are other options ...
            ――
            View topic http://list.abrf.org/r/topic/34QaaQAFJeqW0i9xRxpxdF
            Leave group <email obscured>?Subject=Unsubscribe



        The information contained in this e-mail message may be privileged,
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applicable federal and state laws. If you are not the intended recipient, or an
authorized representative of the intended recipient, any further review,
disclosure, use, dissemination, distribution, or copying of this message or any
attachment (or the information contained therein) is strictly prohibited. If
you think that you have received this e-mail message in error, please notify
the sender by return e-mail and delete all references to it and its contents
from your systems.

        ――
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authorized representative of the intended recipient, any further review,
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