I'm so glad you are attending. We have to make time for a drink. Frances ZRC 1563 Office 646-888-2354 Mobile 516-967-0656 On 1/22/20, 1:01 PM, "Core Rigor and Reproducibility (CoRRe) on behalf of Bover,Laura" <email obscured> on behalf of <email obscured>> wrote: Thanks for the clarification! Obviously I was thinking in my type of experiments, trying to identify new markers upon treatment with certain agents. We used to have a Core here for identification of cell lines, but I am not sure of it is still operating. Thanks! And see you soon at ABRF meeting! Laura Laura Bover, PhD Professor Director Monoclonal Antibody Core Facility University of Texas M.D.Anderson Cancer Center Associate member of The University of Texas Graduate School of Biomedical Sciences at Houston Member of the Scientific Review Committee 2 (former Clinical Research Committee 2) Member of the Steering Committee of the Immunology Program GSBS Immunology Department/ Genomics Medicine Department 7455 Fannin St Room 1SCR4.2021 Houston- TX 77054 T 713-563-3301 <tel:713-563-3301> F 713-563-3276 <tel:713-563-3276> <email obscured> On 1/21/20, 11:22 PM, "Core Rigor and Reproducibility (CoRRe) on behalf of Frances Weis-Garcia" <email obscured> on behalf of <email obscured>> wrote: Thanks Laura and sorry for the confusion. What I am looking for is a way to tell cell line apart if they come from the same parent. For human lines that are from different people, STR profiles are perfect ... but for lines derived from the same original (parent) line ... there is currently no easy way to tell the difference between parent line A and children lines A1, A2 much less grandchild A1.3. Make sense ... If it was a hybridoma, I would just sequence the CDRs. A colleague of mine and I were thinking of inserting a "DNA barcode" in the lines, probably via CRISPR so we can put in as innocuous a site as possible, but before developing a solving I was really hoping one already existed ... so I thought I would poll this group ... All about cell line authentication ... one of the many building blocks needed for RnR. Frances ZRC 1563 Office 646-888-2354 Mobile 516-967-0656 On 1/21/20, 7:39 PM, "Core Rigor and Reproducibility (CoRRe) on behalf of Bover,Laura" <email obscured> on behalf of <email obscured>> wrote: So, do you need to differentiate the parent cells from the ones resulted from the division or proliferating? May be is not your question. What about staining with CFSE a bunch of cells and let them to divide and sort the different peaks (usually 7, at least in normal cells such as T cells proliferating) by FACS. The mean fluorescence as you know is going lower with each division. Is that what you need? Depending on the proliferation time you will be able to separate the last progeny, as well as the intermediate. In normal T cells after 5 days you can recover cells from each division. Not sure if with a cell line you could do the same. Laura Bover, PhD Professor Director Monoclonal Antibody Core Facility University of Texas M.D.Anderson Cancer Center Associate member of The University of Texas Graduate School of Biomedical Sciences at Houston Member of the Scientific Review Committee 2 (former CRC 2) Member of the Steering Committee of the Immunology Program GSBS Immunology Department/ Genomics Medicine Department 7455 Fannin St Room 1SCR4.2021 Houston- TX 77054 T 713-563-3301 <tel:713-563-3301> F 713-563-3276 <tel:713-563-3276> <email obscured> In order to continue obtaining Core support in a challenging environment, it is crucial that our users acknowledge the following grants in all publications (see following text). Thank you! “This work was supported in part by CCSG grant number P30 CA016672.” iLab-Solutions links: https://mdanderson.ilabsolutions.com/service_center/show_external/3631 or: https://mdanderson.ilabsolutions.com/account/login If you do not already have an iLab account please create one prior to sample submission On 1/21/20, 8:15 AM, "Core Rigor and Reproducibility (CoRRe) on behalf of Frances Weis-Garcia" <email obscured> on behalf of <email obscured>> wrote: WARNING: This email originated from outside of MD Anderson. Please validate the sender's email address before clicking on links or attachments as they may not be safe. Question ... Does anyone know of a way to reliably differentiate between decedents / children of a cell line as well as the parent? 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