Hello All,
Has your institution outsourced Next-Gen and/or Sanger sequencing? Are you
currently considering this?
If so, I'd be very appreciative of any insights you're willing to offer. I'm
particularly interested to know whether you've maintained any level of support
(library prep, consultation, data analysis, QC) in-house vs. outsourcing all
genomics services.
Thank you in advance for sharing.
Tom Colella | Senior Director, Core Facilities
Arizona State University | Office of Knowledge Enterprise Development |
Operations
t 480.965.6298 | f 480.965.9811
<email obscured><email obscured>> | https://cores.research.asu.edu/
With Baylor Human Genome Sequencing Center across the way, yes, MD Anderson did
out-source whole genome sequencing. Our core did the QC before sending samples
and worked with HGSC to get the results. We have since brought NGS whole
genome sequencing back in-house because we now have capacity and our samples
are not "production" enough to work well in their pipeline.
Rest of post
-----Original Message-----
From: Core Administrators Network Forum <email obscured>] On Behalf
Of Thomas Colella
Sent: Tuesday, September 11, 2018 5:40 PM
To: CAN-CC <email obscured>) <email obscured>>
Subject: [core administrators network forum] Outsourcing Sequencing?
Hello All,
Has your institution outsourced Next-Gen and/or Sanger sequencing? Are you
currently considering this?
If so, I'd be very appreciative of any insights you're willing to offer. I'm
particularly interested to know whether you've maintained any level of support
(library prep, consultation, data analysis, QC) in-house vs. outsourcing all
genomics services.
Thank you in advance for sharing.
Tom Colella | Senior Director, Core Facilities Arizona State University |
Office of Knowledge Enterprise Development | Operations t 480.965.6298 | f
480.965.9811 <email obscured><email obscured>> |
https://cores.research.asu.edu/
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Hi Tom,
We perform NGS (including Next Seq and Single Cell) in house. UNMC is the 'go
to' NGS resource for the NU system (4 campuses) and so we get quite a bit of
business from UNL. However, I will note that there are researchers at both the
Omaha and Lincoln campuses who send NGS samples out.
Regarding Sanger, this year (July) we began outsourcing capillary sequencing to
GeneWiz. However, the core is the drop off point for samples and the core
emails the results back to the researchers. This way the core scientists
maintain their "expert" status. So far this seems to be working well. The core
does not perform any QC of the samples prior to sending them off to Genewiz.
Genewiz provides detailed protocols for preparation of different sample types.
Rest of post
_________________________________________________________________
Paula B. Turpen, PhD
Director, Research Resources
Office of the Vice Chancellor for Research
University of Nebraska Medical Center
987878 Nebraska Medical Center | Omaha, NE 68198
402.559.6162 | fax 402.559.3300
<email obscured>
-----Original Message-----
From: Core Administrators Network Forum <email obscured>> On Behalf Of
Thomas Colella
Sent: Tuesday, September 11, 2018 5:40 PM
To: CAN-CC <email obscured>) <email obscured>>
Subject: [core administrators network forum] Outsourcing Sequencing?
Hello All,
Has your institution outsourced Next-Gen and/or Sanger sequencing? Are you
currently considering this?
If so, I'd be very appreciative of any insights you're willing to offer. I'm
particularly interested to know whether you've maintained any level of support
(library prep, consultation, data analysis, QC) in-house vs. outsourcing all
genomics services.
Thank you in advance for sharing.
Tom Colella | Senior Director, Core Facilities Arizona State University |
Office of Knowledge Enterprise Development | Operations t 480.965.6298 | f
480.965.9811 <email obscured><email obscured>> |
https://cores.research.asu.edu/
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please delete it and immediately contact the sender.
Hi Tom,
At Northwestern we outsourced Sanger sequencing a couple of years ago to a
local company, ACGT Inc. Their IT group was willing to work with us so we built
an interface that allows our core management software (NUcore) to offer a
replica of the ACGT order form, though the order is transmitted to and
fulfilled by ACGT. We have collection points on each campus for samples and the
core ships them daily. Users get an email with a link from ACGT when their data
is ready. They download the data directly from ACGT.
There's been some complaining (no solution satisfies everyone) but by and large
it works pretty well.
We do NGS in house.
Rest of post
Thanks.
Jeff
-----Original Message-----
From: Core Administrators Network Forum <email obscured>> On Behalf Of
Thomas Colella
Sent: Tuesday, September 11, 2018 5:40 PM
To: CAN-CC <email obscured>) <email obscured>>
Subject: [core administrators network forum] Outsourcing Sequencing?
Hello All,
Has your institution outsourced Next-Gen and/or Sanger sequencing? Are you
currently considering this?
If so, I'd be very appreciative of any insights you're willing to offer. I'm
particularly interested to know whether you've maintained any level of support
(library prep, consultation, data analysis, QC) in-house vs. outsourcing all
genomics services.
Thank you in advance for sharing.
Tom Colella | Senior Director, Core Facilities Arizona State University |
Office of Knowledge Enterprise Development | Operations t 480.965.6298 | f
480.965.9811 <email obscured><email obscured>> |
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