mAb sequencing

Hi all,
I know about 5 years back we discussed doing a new study on antibody
sequencing.  Well, I'm in the process of starting to do that and I am reaching
out for guidance, primers, etc.  We will only be doing murine stuff right now,
but any advice would be appreciated!
All my best,
Scott

Scott Brown, Ph.D.
Director of Immunologic Reagent Facility
Department of Immunology
St. Jude Children's Res. Hosp.
w. (901) 595-4067
c. (901) 674-9960

Rest of post



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Email Disclaimer: www.stjude.org/emaildisclaimer
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Scott,

Thanks for the note.  There is a kit from Novagen (Mouse Ig-Primer, cat #
69831-3) with murine primers.  There are a couple of papers with primers
that people have tried that I will try and dig up.

David

On Fri, Apr 16, 2021 at 11:46 AM Brown, Scott A <

Rest of post

<email obscured>> wrote:

> Hi all,
> I know about 5 years back we discussed doing a new study on antibody
> sequencing.  Well, I'm in the process of starting to do that and I am
> reaching out for guidance, primers, etc.  We will only be doing murine
> stuff right now, but any advice would be appreciated!
> All my best,
> Scott
>
> Scott Brown, Ph.D.
> Director of Immunologic Reagent Facility
> Department of Immunology
> St. Jude Children's Res. Hosp.
> w. (901) 595-4067
> c. (901) 674-9960
>
>
>
> ________________________________
>
> Email Disclaimer: www.stjude.org/emaildisclaimer
> Consultation Disclaimer: www.stjude.org/consultationdisclaimer
>
> ――
> View topic http://list.abrf.org/r/topic/3TVhR3uBTkrXbyWv5qvPKz
> Leave group <email obscured>?Subject=Unsubscribe
>
>

Thanks David!

Scott

Scott Brown, Ph.D.
Director of Immunologic Reagent Facility
Department of Immunology
St. Jude Children’s Research Hospital
262 Danny Thomas Place
Memphis, TN 38105

Rest of post

________________________________
From: Antibody Discussion Forum <email obscured>> on behalf of David
Blum <email obscured>>
Sent: Monday, April 19, 2021 10:01:09 AM
To: <email obscured> <email obscured>>
Subject: Re: [ABRF Ab Forum] mAb sequencing

Caution: External Sender. Do not open unless you know the content is safe.


Scott,

Thanks for the note.  There is a kit from Novagen (Mouse Ig-Primer, cat #
69831-3) with murine primers.  There are a couple of papers with primers
that people have tried that I will try and dig up.

David

On Fri, Apr 16, 2021 at 11:46 AM Brown, Scott A <
<email obscured>> wrote:

> Hi all,
> I know about 5 years back we discussed doing a new study on antibody
> sequencing.  Well, I'm in the process of starting to do that and I am
> reaching out for guidance, primers, etc.  We will only be doing murine
> stuff right now, but any advice would be appreciated!
> All my best,
> Scott
>
> Scott Brown, Ph.D.
> Director of Immunologic Reagent Facility
> Department of Immunology
> St. Jude Children's Res. Hosp.
> w. (901) 595-4067
> c. (901) 674-9960
>
>
>
> ________________________________
>
> Email Disclaimer:
www.stjude.org/emaildisclaimer<http://www.stjude.org/emaildisclaimer>
> Consultation Disclaimer:
www.stjude.org/consultationdisclaimer<http://www.stjude.org/consultationdisclaimer>
>
> ――
> View topic
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Here is the paper where the authors developed their own primers.

https://pubmed.ncbi.nlm.nih.gov/10648866/


On Mon, Apr 19, 2021 at 11:08 AM Brown, Scott A <

Rest of post

<email obscured>> wrote:

> Thanks David!
>
> Scott
>
> Scott Brown, Ph.D.
> Director of Immunologic Reagent Facility
> Department of Immunology
> St. Jude Children’s Research Hospital
> 262 Danny Thomas Place
> Memphis, TN 38105
> ________________________________
> From: Antibody Discussion Forum <email obscured>> on behalf of
> David Blum <email obscured>>
> Sent: Monday, April 19, 2021 10:01:09 AM
> To: <email obscured> <email obscured>>
> Subject: Re: [ABRF Ab Forum] mAb sequencing
>
> Caution: External Sender. Do not open unless you know the content is safe.
>
>
> Scott,
>
> Thanks for the note.  There is a kit from Novagen (Mouse Ig-Primer, cat #
> 69831-3) with murine primers.  There are a couple of papers with primers
> that people have tried that I will try and dig up.
>
> David
>
> On Fri, Apr 16, 2021 at 11:46 AM Brown, Scott A <
> <email obscured>> wrote:
>
> > Hi all,
> > I know about 5 years back we discussed doing a new study on antibody
> > sequencing.  Well, I'm in the process of starting to do that and I am
> > reaching out for guidance, primers, etc.  We will only be doing murine
> > stuff right now, but any advice would be appreciated!
> > All my best,
> > Scott
> >
> > Scott Brown, Ph.D.
> > Director of Immunologic Reagent Facility
> > Department of Immunology
> > St. Jude Children's Res. Hosp.
> > w. (901) 595-4067
> > c. (901) 674-9960
> >
> >
> >
> > ________________________________
> >
> > Email Disclaimer: www.stjude.org/emaildisclaimer<
> http://www.stjude.org/emaildisclaimer>
> > Consultation Disclaimer: www.stjude.org/consultationdisclaimer<
> http://www.stjude.org/consultationdisclaimer>
> >
> > ――
> > View topic
>
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mAb sequencing

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