--
If you have used any of JAX’s Scientific Services please remember to
acknowledge the Service(s) used, in all manuscripts, publications, grant
applications, press releases, and presentations.
Sample language: We gratefully acknowledge the contribution of [optional: name
of person] and the [insert Service’s name] Service at The Jackson Laboratory
for expert assistance with the work described in this publication.
Alan Sawyer
Senior Director, Scientific Services
The Jackson Laboratory
600 Main Street
Bar Harbor, ME 04609
207-288-6978
<email obscured><email obscured>>
www.jax.org
https://orcid.org/0000-0001-5942-4498
Louise Lopez
Office Administrator, Scientific Services
The Jackson Laboratory
600 Main Street, Bar Harbor, Maine 04609
P: 207-288-6484 ~ F: 207-288-6044 ~ C: 207-266-1419
The Jackson Laboratory: Leading the search for tomorrow’s cures
--
On May 25, 2020, at 14:28, Bover,Laura
<email obscured><email obscured>>> wrote:
Hi Frances! Hope you and the group are well and hanging there!
Same than Ed. Denatured by boiling and use for injection and coat. Sometimes I
alternate injections: boil or not, in case I want to get the two options.
One interesting alternative that I found for protein coated directly on the
plate (meaning "flat"?) is to use the his tag version of the protein and side
by side regular plates and Niquel coated plates (commercial).
The first time I asked my technician to do it, he was panicking because
although some of the wells showed identical OD, other showed very different and
some were negative/positive depending on the plate.
So, I use those plates for some projects, not as an alternative but
complementary.
Laura Bover
________________________________
From: Antibody Discussion Forum
<email obscured><email obscured>>> on behalf of
Greenfield, Edward A.,Ph.D.
<email obscured><email obscured>>>
Sent: Monday, May 25, 2020 8:22 AM
To: <email obscured><email obscured>>
Subject: [EXT] Re: [ABRF Ab Forum] Denature proteins
WARNING: This email originated from outside of MD Anderson. Please validate the
sender's email address before clicking on links or attachments as they may not
be safe.
Hi Frances -
The last few projects I have had to make antibodies to native (for FACS) and
denatured (for WB) protein I have found immunizing with the protein after
boiling it for about 1 minute has been effective for denaturing. The boiled
protein can be bound to ELISA plates or used in Dot-Blots.
Best,
Ed.
-----Original Message-----
From: Antibody Discussion Forum
<email obscured><email obscured>>> On Behalf Of Frances
Weis-Garcia
Sent: Friday, May 22, 2020 3:09 PM
To: ABRF Forum <email obscured><email obscured>>>
Subject: [ABRF Ab Forum] Denature proteins
External Email - Use Caution
Yes. … it’s me again 😉
Looking for a protocol by which to screen denatured proteins. I have a really
nicely folded one and would like to see the antibody response against it and a
denatured version of it. Anyone have protocol they like.
I know an antibody core used to use a Urea protocol but they closed ☹.
Frances
――
View topic
https://urldefense.com/v3/__http://list.abrf.org/r/topic/7AkB1zFTdvJPWmCTQaMP7b__;!!PfbeBCCAmug!1491ZH_anwrpqZRVUoJMHtG4iOrQMULaCXWW2WLeqvuxeAayuHXq7Yw0L3t2r8BW$
Denature proteins: Antibody Discussion Forum: ABRF Discussion
Forums<https://urldefense.com/v3/__http://list.abrf.org/r/topic/7AkB1zFTdvJPWmCTQaMP7b__;!!PfbeBCCAmug!1491ZH_anwrpqZRVUoJMHtG4iOrQMULaCXWW2WLeqvuxeAayuHXq7Yw0L3t2r8BW$>
urldefense.com<http://urldefense.com>
Yes. … it’s me again 😉 Looking for a protocol by which to screen denatured
proteins. I have a really nicely folded one and would like to see the antibody
re
Leave group <email obscured>?Subject=Unsubscribe
The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine
at
https://urldefense.com/v3/__http://www.partners.org/complianceline__;!!PfbeBCCAmug!1491ZH_anwrpqZRVUoJMHtG4iOrQMULaCXWW2WLeqvuxeAayuHXq7Yw0L7XNMw0i$
. If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and
properly
dispose of the e-mail.
――
View topic
https://urldefense.com/v3/__http://list.abrf.org/r/topic/3c8scM7l3A7LgSIXZrdpcn__;!!PfbeBCCAmug!1491ZH_anwrpqZRVUoJMHtG4iOrQMULaCXWW2WLeqvuxeAayuHXq7Yw0L_BCGaHo$
Leave group <email obscured>?Subject=Unsubscribe
The information contained in this e-mail message may be privileged,
confidential, and/or protected from disclosure. This e-mail message may contain
protected health information (PHI); dissemination of PHI should comply with
applicable federal and state laws. If you are not the intended recipient, or an
authorized representative of the intended recipient, any further review,
disclosure, use, dissemination, distribution, or copying of this message or any
attachment (or the information contained therein) is strictly prohibited. If
you think that you have received this e-mail message in error, please notify
the sender by return e-mail and delete all references to it and its contents
from your systems.
――
View topic http://list.abrf.org/r/topic/5ZUqOZDQCGO7yYlCO5GAFU
Leave group <email obscured>?Subject=Unsubscribe
---
The information in this email, including attachments, may be confidential and
is intended solely for the addressee(s). If you believe you received this email
by mistake, please notify the sender by return email as soon as possible.
