Posts in Antibody Discussion Forum

On Thu, Jul 9, 2020 at 10:31 AM David Blum <email obscured>> wrote:

> [image: Picture1.png]
>
>
>
> *COVID-19 Serological Assay Design:  Lessons Learned*
>
>
>
>
>
> *ABRF Antibody Interest Group Presentation Monday, July 27, 2020 11:00
> a.m.–12:00 p.m. ET *
>
> Join Zoom session
> <https://us02web.zoom.us/j/85207659502?pwd=c1FzRnJqYVQyUkdjSUNzeTRQQ0NmQT09>
>
>
>
> Testing COVID-19 patients and asymptomatic people for active infection
> using RT-PCR and past infection with a serological test are central to
> understanding and managing the disease in a population.  Core Scientists
> in the ABRF Antibody Interest Group have been developing ELISAs to help
> their institutions study SARS-CoV-2 seroconversion and will share the
> lessons they have learned along the way.
>
>
>
> Join us for this timely and informative presentation.
>
>
>
> Moderator:
>
>
>
> *David Blum, Ph.D.*
>
> Director, Bioexpression and Fermentation Facility
>
> Director, Master of Biomanufacturing and Bioprocessing
>
> University of Georgia
>
>
>
> Speakers:
>
>
>
> *Scott Brown, Ph.D.*
>
> Director, Immunologic Reagent Facility
>
> Department of Immunology
>
> St. Jude Children’s Research Hospital
>
>
>
> *Frances Weis-Garcia, Ph.D.*
>
> Head, Bi-Institutional Antibody and Bioresource Core Facility
>
> Memorial Sloan Kettering Cancer Center
>
> The Rockefeller University
>
>
>

<email obscured>> wrote:

> Hi!
>
> I would be interested in this virtual lab meeting.
>
>  Best,
>
> Thierry Le Bihan, Ph.D.
> Senior Research Scientist
> <email obscured>
> 1-855-899-9990 ext 705
> Unit 450, 137 Glasgow St
> Kitchener, ON, Canada N2G 4X8
>
> *Rapid Novor, Inc ● Kitchener ● N2G 4X8 ● ON ● Canada ● www.rapidnovor.com
> <http://www.rapidnovor.com/>*
> *This electronic message contains information from Rapid Novor, Inc. The
> contents may be privileged and confidential and are intended for the use of
> the intended addressee(s) only.  If you are not an intended addressee, note
> that any disclosure, copying, distribution, or use of the contents of this
> message is prohibited. If you have received this e-mail in error, please
> notify the sender immediately and delete this email. Thank you.*
>
>
>
> ---------- Forwarded message ---------
> From: David Blum <email obscured>>
> Date: Mon, Jun 29, 2020 at 2:30 PM
> Subject: Re: [ABRF Ab Forum] FW: [EXT] Re: [core rigor and reproducibility
> (corre)] We have an RRID number
> To: <email obscured>>
>
>
> We had a great Virtual Lab Meeting today.  One of the outcomes is that we
> are planning to restart the VLMs.  The first one will be on July 27th at
> 11am EDT.  Frances Weis-Garcia and Scott Brown will be discussing COVID-19
> serology assays.  Click on the link below for the next speakers which have
> been scheduled for August and September.  If you or someone you know has a
> topic to present, please add your name to the list for October, November or
> December.  I will send out information for the call as we get closer to the
> date.
>
>
>
https://docs.google.com/spreadsheets/d/1dDiEGT3ImKbO6pZ8R29HX7Z4sG7QHEuecof7gf7ig3g/edit?usp=sharing
>
>
>
> On Thu, Jun 25, 2020 at 8:55 AM Christensen, Kurt Richard <
> <email obscured>>
> wrote:
>
> > Hi All,
> > I would be interested also.
> > Thanks,
> > Kurt
> >
> > Kurt Christensen
> > Lab Manager, Protein and Monoclonal Antibody Production Shared Resource
> > Baylor College of Medicine
> > Dan L. Duncan Cancer Center
> > Mail Stop BCM130, Rm R551E
> > 1 Baylor Plaza
> > Houston, TX  77030
> > Office: 713-798-2325
> > Lab:713-798-2327
> > Email: <email obscured>
> > Web:
> >
>
>
https://www.bcm.edu/centers/cancer-center/research/shared-resources/antibody-based-proteomics/recombinant-protein-monoclonal-antibody
> > iLab Service Requests:
> > https://bcm.corefacilities.org/account/login
> > iLab FAQs:
> >
> >
>
>
https://www.bcm.edu/research/advanced-technology-core-labs/lab-listing/ilanfaq/ilabfaqcustomer
> >
> > -----Original Message-----
> > From: Antibody Discussion Forum <email obscured>> On Behalf Of
> > David Blum
> > Sent: Wednesday, June 24, 2020 8:24 PM
> > To: <email obscured>
> > Subject: Re: [ABRF Ab Forum] FW: [EXT] Re: [core rigor and
> reproducibility
> > (corre)] We have an RRID number
> >
> > I sent out an email with the Zoom details and can add anybody else that
> > responds to this thread.
> >
> > On Wed, Jun 24, 2020 at 8:04 PM Greenfield, Edward A.,Ph.D. <
> > <email obscured>> wrote:
> >
> > > I would be interested.
> > >
> > > Best,
> > > Ed.
> > >
> > > -----Original Message-----
> > > From: Antibody Discussion Forum <email obscured>> On Behalf Of
> > > David Blum
> > > Sent: Wednesday, June 24, 2020 4:15 PM
> > > To: <email obscured>
> > > Subject: Re: [ABRF Ab Forum] FW: [EXT] Re: [core rigor and
> > > reproducibility (corre)] We have an RRID number
> > >
> > >         External Email - Use Caution
> > >
> > > Laura,
> > >
> > > Thanks for the note.  As a group, we haven't been active, but one
> > > thing we did was the Virtual Lab Meeting (VLM).  I would be happy to
> > > setup a zoom teleconference and anyone that would like to join could.
> > > Perhaps the first call could be for strategic planning, discussing
> > > what we would like to accomplish then setup subsequent calls after
> > > that.  Previously the calls were held at 11am EDT to accommodate people
> > in Europe and on the West coast.
> > >
> > > Would anyone be interested in me setting up a call next Monday June
> > > 29th at 11am EDT?
> > >
> > > On Tue, Jun 23, 2020 at 10:12 PM Bover,Laura <email obscured>>
> > > wrote:
> > >
> > > > Dear antibody guys,
> > > > I hope all of you are doing fine.
> > > > We are back in business since June 16th. Not sure for how long
> > > > because Texas is raising in cases: today, Gov. Greg Abbott shared
> > > > that Texas has seen 5,000 new coronavirus cases in a single day for
> > > > the first time, the numbers are moving fast in the wrong direction.
> > > > Our lab works, as probably yours, based on NCI funds and program
> > income.
> > > > Thus, as far as we can continue to work we are fine.
> > > >
> > > > On the other hand, this virus has made a breakthrough: everybody
> > > > realize that immune system exists, and  everybody wants antibodies
> > > > against everything. People that never work in antibodies titration
> > > > before are titrating antibodies in plasma from recovered patients
> > > > and publishing a Preprint with one of the main figures a graph of an
> > > > ELISA!! Things that we usually only mention in Mat an Meth.
> > > >
> > > > With all that said I proposed Frances, if you would like to meet,
> > > > once a month or once in a time, and see how are all you doing, how
> > > > this is progressing and if you have contingencies plans in case you
> > > > cannot work. Or simple share a good moment, or news or papers, being
> > > > the case that one of the main treatments is so far convalescent
> > > > plasma and hyperimmune polyclonal antibodies obtained and purified
> > > > from horses, recombinant from llamas and eventually cloned from
> > > > single B cells from recovered donors to use as poolsof antibodies.
> > > >
> > > > Approaches for vaccines developments. In fact all things that we are
> > > > the experts! Immunizing to get immune response!
> > > > May be write a review, talk , etc?
> > > >
> > > > Also I would like to know in a personal question is, how would my
> > > > lab get a CLIA certification for making an ELISA for antibodies
> > > > titration in this case, instead than a researcher that never worked
> > > > with this, takes it as a potential result that may affect other's
> > lives.
> > > >
> > > > What do you think?
> > > > Can we do anything as a community of experts??
> > > > Thanks!!
> > > >
> > > > Laura
> > > > PS I would love to use zoom for these meetings and I am still
> > > > thinking why the heck I didn't invest in Zoom's stocks!!
> > > >
> > > > Laura Bover, PhD
> > > > Professor
> > > > Director Monoclonal Antibody Core Facility University of Texas
> > > > M.D.Anderson Cancer Center Associate member of The University of
> > > > Texas Graduate School of Biomedical Sciences at Houston Member of
> > > > the Scientific Review Committee 2 (former Clinical Research
> > > > Committee 2) Member of the Steering Committee of the Immunology
> > > > Program GSBS Immunology Department/ Genomics Medicine Department
> > > > 7455 Fannin St Room 1SCR4.2021
> > > > Houston- TX 77054
> > > > T  713-563-3301 <tel:713-563-3301>
> > > > F 713-563-3276 <tel:713-563-3276>
> > > > <email obscured>
> > > >
> > > > In order to continue obtaining Core support in a challenging
> > > > environment, it is crucial that our users acknowledge the following
> > > > grants in all publications (see following text).  Thank you!
> > > >  “We acknowledge the UT-MDACC Monoclonal Antibodies Core facility,
> > > > supported in part by CCSG grant number P30 CA016672.”
> > > >
> > > >
> > > >
> > > >
> > > >
> > > > On 6/23/20, 2:11 PM, "Frances Weis-Garcia" <
> > > > <email obscured>> wrote:
> > > >
> > > >     WARNING: This email originated from outside of MD Anderson.
> > > > Please validate the sender's email address before clicking on links
> > > > or attachments as they may not be safe.
> > > >
> > > >     I would love a monthly pow wow.
> > > >     Please post to the list ... and let's see who is game.  It think
> > > > you get a few takers.
> > > >
> > > >
> > > >     On 6/23/20, 1:07 PM, "Bover,Laura" <email obscured>>
> wrote:
> > > >
> > > >         Hi Frances, I have just seen this email hidden under 200! I
> > > > will read it and I will probably go ask with questions.
> > > >         Should we organize a monthly meeting for MAbs group? Just to
> > > > exchange situations in other Cores and states and cities and
> > > institutions?
> > > >
> > > >         One thing, I would like to know how a Core can have a
> > > > certification CLIA is it is developed an assay for diagnostic. Let's
> > > > say an ELISA, is that possible?
> > > >         Thanks!
> > > >         Laura
> > > >
> > > >         On 6/2/20, 10:52 AM, "Core Rigor and Reproducibility (CoRRe)
> > > > on behalf of Frances Weis-Garcia" <email obscured> on behalf of
> > > > <email obscured>> wrote:
> > > >
> > > >             WARNING: This email originated from outside of MD
> Anderson.
> > > > Please validate the sender's email address before clicking on links
> > > > or attachments as they may not be safe.
> > > >
> > > >             ― 1 file link ―
> > > >
> > > >             Found quite a few have been given for a few at MSKCC …
> > > > for example.
> > > >
> > > >
> > > >
> > > >               *   SCR_017850<
> > > > https://urldefense.com/v3/__https://scicrunch.org/resources/Any/sear
> > > > ch
> > > > ?q=SCR_017850&l=SCR_017850__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65l
> > > > I9 i3lotEEL46o4XMF7Xe17We2_0sMhA716G-kwD$
> > > > > - Molecular Cytogenetics Core Facility
> > > >               *   SCR_017691<
> > > > https://urldefense.com/v3/__https://scicrunch.org/resources/Any/sear
> > > > ch
> > > > ?q=SCR_017691&l=SCR_017691__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65l
> > > > I9 i3lotEEL46o4XMF7Xe17We2_0sMhA7_em4O2l$
> > > > > - Antibody & Bioresource Core Facility
> > > >               *   SCR_017853<
> > > > https://urldefense.com/v3/__https://scicrunch.org/resources/Any/sear
> > > > ch
> > > > ?q=SCR_017853&l=SCR_017853__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65l
> > > > I9 i3lotEEL46o4XMF7Xe17We2_0sMhA79oyOBYh$
> > > > > - Investigational Products Support Core Facility
> > > >
> > > >             From: Frances Weis-Garcia <
> <email obscured>
> > >
> > > >             Date: Tuesday, June 2, 2020 at 11:48 AM
> > > >             To: "Core Rigor and Reproducibility (CoRRe)" <
> > > > <email obscured>>
> > > >             Subject: We have an RRID number
> > > >
> > > >             We have an RRID number
> > > >             But cannot find other cores with RRID numbers (yet)
> > > >
> > > >             Only matters if we want to define the structure of the
> > > > core RRID
> > > >
> > > >             [A screenshot of a cell phone  Description automatically
> > > > generated]
> > > >
> > > >             ― 1 file ―
> > > >
> > > >               □ image001.png  (386kb)
> > > >
> > > > https://urldefense.com/v3/__http://list.abrf.org/r/file/61rKfXKRRhmc
> > > > eb
> > > > jVxMqaDd73I7M-1CvB-2NFYRPN/__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65
> > > > lI 9i3lotEEL46o4XMF7Xe17We2_0sMhA7-cq3rnW$
> > > >
> > > >             ――
> > > >             View topic
> > > >
> > > https://urldefense.com/v3/__http://list.abrf.org/r/topic/5kRaozb5cnXRg
> > > NPMB40Ica__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65lI9i3lotEEL46o4XMF7X
> > > e17We2_0sMhA77Xsei7H$
> > > >             Leave group
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> > > >
> > > >
> > > >
> > > >
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>

> Hi All,
> I would be interested also.
> Thanks,
> Kurt
>
> Kurt Christensen
> Lab Manager, Protein and Monoclonal Antibody Production Shared Resource
> Baylor College of Medicine
> Dan L. Duncan Cancer Center
> Mail Stop BCM130, Rm R551E
> 1 Baylor Plaza
> Houston, TX  77030
> Office: 713-798-2325
> Lab:713-798-2327
> Email: <email obscured>
> Web:
>
https://www.bcm.edu/centers/cancer-center/research/shared-resources/antibody-based-proteomics/recombinant-protein-monoclonal-antibody
> iLab Service Requests:
> https://bcm.corefacilities.org/account/login
> iLab FAQs:
>
>
https://www.bcm.edu/research/advanced-technology-core-labs/lab-listing/ilanfaq/ilabfaqcustomer
>
> -----Original Message-----
> From: Antibody Discussion Forum <email obscured>> On Behalf Of
> David Blum
> Sent: Wednesday, June 24, 2020 8:24 PM
> To: <email obscured>
> Subject: Re: [ABRF Ab Forum] FW: [EXT] Re: [core rigor and reproducibility
> (corre)] We have an RRID number
>
> I sent out an email with the Zoom details and can add anybody else that
> responds to this thread.
>
> On Wed, Jun 24, 2020 at 8:04 PM Greenfield, Edward A.,Ph.D. <
> <email obscured>> wrote:
>
> > I would be interested.
> >
> > Best,
> > Ed.
> >
> > -----Original Message-----
> > From: Antibody Discussion Forum <email obscured>> On Behalf Of
> > David Blum
> > Sent: Wednesday, June 24, 2020 4:15 PM
> > To: <email obscured>
> > Subject: Re: [ABRF Ab Forum] FW: [EXT] Re: [core rigor and
> > reproducibility (corre)] We have an RRID number
> >
> >         External Email - Use Caution
> >
> > Laura,
> >
> > Thanks for the note.  As a group, we haven't been active, but one
> > thing we did was the Virtual Lab Meeting (VLM).  I would be happy to
> > setup a zoom teleconference and anyone that would like to join could.
> > Perhaps the first call could be for strategic planning, discussing
> > what we would like to accomplish then setup subsequent calls after
> > that.  Previously the calls were held at 11am EDT to accommodate people
> in Europe and on the West coast.
> >
> > Would anyone be interested in me setting up a call next Monday June
> > 29th at 11am EDT?
> >
> > On Tue, Jun 23, 2020 at 10:12 PM Bover,Laura <email obscured>>
> > wrote:
> >
> > > Dear antibody guys,
> > > I hope all of you are doing fine.
> > > We are back in business since June 16th. Not sure for how long
> > > because Texas is raising in cases: today, Gov. Greg Abbott shared
> > > that Texas has seen 5,000 new coronavirus cases in a single day for
> > > the first time, the numbers are moving fast in the wrong direction.
> > > Our lab works, as probably yours, based on NCI funds and program
> income.
> > > Thus, as far as we can continue to work we are fine.
> > >
> > > On the other hand, this virus has made a breakthrough: everybody
> > > realize that immune system exists, and  everybody wants antibodies
> > > against everything. People that never work in antibodies titration
> > > before are titrating antibodies in plasma from recovered patients
> > > and publishing a Preprint with one of the main figures a graph of an
> > > ELISA!! Things that we usually only mention in Mat an Meth.
> > >
> > > With all that said I proposed Frances, if you would like to meet,
> > > once a month or once in a time, and see how are all you doing, how
> > > this is progressing and if you have contingencies plans in case you
> > > cannot work. Or simple share a good moment, or news or papers, being
> > > the case that one of the main treatments is so far convalescent
> > > plasma and hyperimmune polyclonal antibodies obtained and purified
> > > from horses, recombinant from llamas and eventually cloned from
> > > single B cells from recovered donors to use as poolsof antibodies.
> > >
> > > Approaches for vaccines developments. In fact all things that we are
> > > the experts! Immunizing to get immune response!
> > > May be write a review, talk , etc?
> > >
> > > Also I would like to know in a personal question is, how would my
> > > lab get a CLIA certification for making an ELISA for antibodies
> > > titration in this case, instead than a researcher that never worked
> > > with this, takes it as a potential result that may affect other's
> lives.
> > >
> > > What do you think?
> > > Can we do anything as a community of experts??
> > > Thanks!!
> > >
> > > Laura
> > > PS I would love to use zoom for these meetings and I am still
> > > thinking why the heck I didn't invest in Zoom's stocks!!
> > >
> > > Laura Bover, PhD
> > > Professor
> > > Director Monoclonal Antibody Core Facility University of Texas
> > > M.D.Anderson Cancer Center Associate member of The University of
> > > Texas Graduate School of Biomedical Sciences at Houston Member of
> > > the Scientific Review Committee 2 (former Clinical Research
> > > Committee 2) Member of the Steering Committee of the Immunology
> > > Program GSBS Immunology Department/ Genomics Medicine Department
> > > 7455 Fannin St Room 1SCR4.2021
> > > Houston- TX 77054
> > > T  713-563-3301 <tel:713-563-3301>
> > > F 713-563-3276 <tel:713-563-3276>
> > > <email obscured>
> > >
> > > In order to continue obtaining Core support in a challenging
> > > environment, it is crucial that our users acknowledge the following
> > > grants in all publications (see following text).  Thank you!
> > >  “We acknowledge the UT-MDACC Monoclonal Antibodies Core facility,
> > > supported in part by CCSG grant number P30 CA016672.”
> > >
> > >
> > >
> > >
> > >
> > > On 6/23/20, 2:11 PM, "Frances Weis-Garcia" <
> > > <email obscured>> wrote:
> > >
> > >     WARNING: This email originated from outside of MD Anderson.
> > > Please validate the sender's email address before clicking on links
> > > or attachments as they may not be safe.
> > >
> > >     I would love a monthly pow wow.
> > >     Please post to the list ... and let's see who is game.  It think
> > > you get a few takers.
> > >
> > >
> > >     On 6/23/20, 1:07 PM, "Bover,Laura" <email obscured>> wrote:
> > >
> > >         Hi Frances, I have just seen this email hidden under 200! I
> > > will read it and I will probably go ask with questions.
> > >         Should we organize a monthly meeting for MAbs group? Just to
> > > exchange situations in other Cores and states and cities and
> > institutions?
> > >
> > >         One thing, I would like to know how a Core can have a
> > > certification CLIA is it is developed an assay for diagnostic. Let's
> > > say an ELISA, is that possible?
> > >         Thanks!
> > >         Laura
> > >
> > >         On 6/2/20, 10:52 AM, "Core Rigor and Reproducibility (CoRRe)
> > > on behalf of Frances Weis-Garcia" <email obscured> on behalf of
> > > <email obscured>> wrote:
> > >
> > >             WARNING: This email originated from outside of MD Anderson.
> > > Please validate the sender's email address before clicking on links
> > > or attachments as they may not be safe.
> > >
> > >             ― 1 file link ―
> > >
> > >             Found quite a few have been given for a few at MSKCC …
> > > for example.
> > >
> > >
> > >
> > >               *   SCR_017850<
> > > https://urldefense.com/v3/__https://scicrunch.org/resources/Any/sear
> > > ch
> > > ?q=SCR_017850&l=SCR_017850__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65l
> > > I9 i3lotEEL46o4XMF7Xe17We2_0sMhA716G-kwD$
> > > > - Molecular Cytogenetics Core Facility
> > >               *   SCR_017691<
> > > https://urldefense.com/v3/__https://scicrunch.org/resources/Any/sear
> > > ch
> > > ?q=SCR_017691&l=SCR_017691__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65l
> > > I9 i3lotEEL46o4XMF7Xe17We2_0sMhA7_em4O2l$
> > > > - Antibody & Bioresource Core Facility
> > >               *   SCR_017853<
> > > https://urldefense.com/v3/__https://scicrunch.org/resources/Any/sear
> > > ch
> > > ?q=SCR_017853&l=SCR_017853__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65l
> > > I9 i3lotEEL46o4XMF7Xe17We2_0sMhA79oyOBYh$
> > > > - Investigational Products Support Core Facility
> > >
> > >             From: Frances Weis-Garcia <email obscured>
> >
> > >             Date: Tuesday, June 2, 2020 at 11:48 AM
> > >             To: "Core Rigor and Reproducibility (CoRRe)" <
> > > <email obscured>>
> > >             Subject: We have an RRID number
> > >
> > >             We have an RRID number
> > >             But cannot find other cores with RRID numbers (yet)
> > >
> > >             Only matters if we want to define the structure of the
> > > core RRID
> > >
> > >             [A screenshot of a cell phone  Description automatically
> > > generated]
> > >
> > >             ― 1 file ―
> > >
> > >               □ image001.png  (386kb)
> > >
> > > https://urldefense.com/v3/__http://list.abrf.org/r/file/61rKfXKRRhmc
> > > eb
> > > jVxMqaDd73I7M-1CvB-2NFYRPN/__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65
> > > lI 9i3lotEEL46o4XMF7Xe17We2_0sMhA7-cq3rnW$
> > >
> > >             ――
> > >             View topic
> > >
> > https://urldefense.com/v3/__http://list.abrf.org/r/topic/5kRaozb5cnXRg
> > NPMB40Ica__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65lI9i3lotEEL46o4XMF7X
> > e17We2_0sMhA77Xsei7H$
> > >             Leave group
> > > <email obscured>?Subject=Unsubscribe
> > >
> > >
> > >
> > >
> > >         The information contained in this e-mail message may be
> > > privileged, confidential, and/or protected from disclosure. This
> > > e-mail message may contain protected health information (PHI);
> > > dissemination of PHI should comply with applicable federal and state
> > > laws. If you are not the intended recipient, or an authorized
> > > representative of the intended recipient, any further review,
> > > disclosure, use, dissemination, distribution, or copying of this
> > > message or any attachment (or the information contained therein) is
> > > strictly prohibited. If you think that you have received this e-mail
> > > message in error, please notify the sender by return e-mail and
> > > delete all references to it and its contents from your systems.
> > >
> > >
> > >
> > >
> > > The information contained in this e-mail message may be privileged,
> > > confidential, and/or protected from disclosure. This e-mail message
> > > may contain protected health information (PHI); dissemination of PHI
> > > should comply with applicable federal and state laws. If you are not
> > > the intended recipient, or an authorized representative of the
> > > intended recipient, any further review, disclosure, use,
> > > dissemination, distribution, or copying of this message or any
> > > attachment (or the information contained therein) is strictly
> > > prohibited. If you think that you have received this e-mail message
> > > in error, please notify the sender by return e-mail and delete all
> > references to it and its contents from your systems.
> > >
> > > ――
> > > View topic
> > > https://urldefense.proofpoint.com/v2/url?u=http-3A__list.abrf.org_r_
> > > topic_5bRUHLyrqMfdch3xJ1egAo&d=DwIFaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=_dq
> > > qB8TU9vN7Hm15OCeZVQ&m=1u0JscGdrLmtrkHhrqe8WwI6NVzgutPR30s1tgjyY_Y&s=
> > > cfvvDUSfzl7P5FUstOu5spgTWua1wjQtUjDEvRIihyY&e=
> > > Leave group <email obscured>?Subject=Unsubscribe
> > >
> > >
> >
> > ――
> > View topic
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__list.abrf.org_r_to
> > pic_7efFJlkEa5AdocqXi4oNC2&d=DwIFaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=_dqqB8T
> > U9vN7Hm15OCeZVQ&m=1u0JscGdrLmtrkHhrqe8WwI6NVzgutPR30s1tgjyY_Y&s=LmYnYw
> > s_n3DWZpvKTJ2P451Erv9uVBK1pmDmRUMZom0&e=
> > Leave group <email obscured>?Subject=Unsubscribe
> >
> >
> >
> > The information in this e-mail is intended only for the person to whom
> > it is addressed. If you believe this e-mail was sent to you in error
> > and the e-mail contains patient information, please contact the
> > Partners Compliance HelpLine at
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__www.partners.org_c
> > omplianceline&d=DwIFaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=_dqqB8TU9vN7Hm15OCeZ
> > VQ&m=1u0JscGdrLmtrkHhrqe8WwI6NVzgutPR30s1tgjyY_Y&s=xEjGmVKK9e2U9jBhm1F
> > sF8s2Ci7jdURmLIHNXlgfHas&e=  . If the e-mail was sent to you in error
> > but does not contain patient information, please contact the sender and
> properly dispose of the e-mail.
> >
> > ――
> > View topic
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__list.abrf.org_r_to
> > pic_seIYKRgv7RlwME9A9WyC9&d=DwIFaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=_dqqB8TU
> > 9vN7Hm15OCeZVQ&m=1u0JscGdrLmtrkHhrqe8WwI6NVzgutPR30s1tgjyY_Y&s=ipzGFUu
> > ScZyavm7S0h3zU_9sgQ9wOgARsOdia3nq0Dg&e=
> > Leave group <email obscured>?Subject=Unsubscribe
> >
> >
>
> ――
> View topic
>
https://urldefense.proofpoint.com/v2/url?u=http-3A__list.abrf.org_r_topic_5UDVSZ9IKBgdxohmZxQlwi&d=DwIFaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=_dqqB8TU9vN7Hm15OCeZVQ&m=1u0JscGdrLmtrkHhrqe8WwI6NVzgutPR30s1tgjyY_Y&s=XqcTqNOsaS3jiPdGxRPjUKa7BkHkAbJbJq77y8s5hK0&e=
> Leave group <email obscured>?Subject=Unsubscribe
>
>
> ――
> View topic http://list.abrf.org/r/topic/4g1Itq5NXiJN4aUBJ5jS2c
> Leave group <email obscured>?Subject=Unsubscribe
>
>

<email obscured>> wrote:

> I would be interested.
>
> Best,
> Ed.
>
> -----Original Message-----
> From: Antibody Discussion Forum <email obscured>> On Behalf Of
> David Blum
> Sent: Wednesday, June 24, 2020 4:15 PM
> To: <email obscured>
> Subject: Re: [ABRF Ab Forum] FW: [EXT] Re: [core rigor and reproducibility
> (corre)] We have an RRID number
>
>         External Email - Use Caution
>
> Laura,
>
> Thanks for the note.  As a group, we haven't been active, but one thing we
> did was the Virtual Lab Meeting (VLM).  I would be happy to setup a zoom
> teleconference and anyone that would like to join could.  Perhaps the first
> call could be for strategic planning, discussing what we would like to
> accomplish then setup subsequent calls after that.  Previously the calls
> were held at 11am EDT to accommodate people in Europe and on the West coast.
>
> Would anyone be interested in me setting up a call next Monday June 29th
> at 11am EDT?
>
> On Tue, Jun 23, 2020 at 10:12 PM Bover,Laura <email obscured>>
> wrote:
>
> > Dear antibody guys,
> > I hope all of you are doing fine.
> > We are back in business since June 16th. Not sure for how long because
> > Texas is raising in cases: today, Gov. Greg Abbott shared that Texas
> > has seen 5,000 new coronavirus cases in a single day for the first
> > time, the numbers are moving fast in the wrong direction.
> > Our lab works, as probably yours, based on NCI funds and program income.
> > Thus, as far as we can continue to work we are fine.
> >
> > On the other hand, this virus has made a breakthrough: everybody
> > realize that immune system exists, and  everybody wants antibodies
> > against everything. People that never work in antibodies titration
> > before are titrating antibodies in plasma from recovered patients and
> > publishing a Preprint with one of the main figures a graph of an
> > ELISA!! Things that we usually only mention in Mat an Meth.
> >
> > With all that said I proposed Frances, if you would like to meet, once
> > a month or once in a time, and see how are all you doing, how this is
> > progressing and if you have contingencies plans in case you cannot
> > work. Or simple share a good moment, or news or papers, being the case
> > that one of the main treatments is so far convalescent plasma and
> > hyperimmune polyclonal antibodies obtained and purified from horses,
> > recombinant from llamas and eventually cloned from single B cells from
> > recovered donors to use as poolsof antibodies.
> >
> > Approaches for vaccines developments. In fact all things that we are
> > the experts! Immunizing to get immune response!
> > May be write a review, talk , etc?
> >
> > Also I would like to know in a personal question is, how would my lab
> > get a CLIA certification for making an ELISA for antibodies titration
> > in this case, instead than a researcher that never worked with this,
> > takes it as a potential result that may affect other's lives.
> >
> > What do you think?
> > Can we do anything as a community of experts??
> > Thanks!!
> >
> > Laura
> > PS I would love to use zoom for these meetings and I am still thinking
> > why the heck I didn't invest in Zoom's stocks!!
> >
> > Laura Bover, PhD
> > Professor
> > Director Monoclonal Antibody Core Facility University of Texas
> > M.D.Anderson Cancer Center Associate member of The University of Texas
> > Graduate School of Biomedical Sciences at Houston Member of the
> > Scientific Review Committee 2 (former Clinical Research Committee 2)
> > Member of the Steering Committee of the Immunology Program GSBS
> > Immunology Department/ Genomics Medicine Department
> > 7455 Fannin St Room 1SCR4.2021
> > Houston- TX 77054
> > T  713-563-3301 <tel:713-563-3301>
> > F 713-563-3276 <tel:713-563-3276>
> > <email obscured>
> >
> > In order to continue obtaining Core support in a challenging
> > environment, it is crucial that our users acknowledge the following
> > grants in all publications (see following text).  Thank you!
> >  “We acknowledge the UT-MDACC Monoclonal Antibodies Core facility,
> > supported in part by CCSG grant number P30 CA016672.”
> >
> >
> >
> >
> >
> > On 6/23/20, 2:11 PM, "Frances Weis-Garcia" <
> > <email obscured>> wrote:
> >
> >     WARNING: This email originated from outside of MD Anderson. Please
> > validate the sender's email address before clicking on links or
> > attachments as they may not be safe.
> >
> >     I would love a monthly pow wow.
> >     Please post to the list ... and let's see who is game.  It think
> > you get a few takers.
> >
> >
> >     On 6/23/20, 1:07 PM, "Bover,Laura" <email obscured>> wrote:
> >
> >         Hi Frances, I have just seen this email hidden under 200! I
> > will read it and I will probably go ask with questions.
> >         Should we organize a monthly meeting for MAbs group? Just to
> > exchange situations in other Cores and states and cities and
> institutions?
> >
> >         One thing, I would like to know how a Core can have a
> > certification CLIA is it is developed an assay for diagnostic. Let's
> > say an ELISA, is that possible?
> >         Thanks!
> >         Laura
> >
> >         On 6/2/20, 10:52 AM, "Core Rigor and Reproducibility (CoRRe)
> > on behalf of Frances Weis-Garcia" <email obscured> on behalf of
> > <email obscured>> wrote:
> >
> >             WARNING: This email originated from outside of MD Anderson.
> > Please validate the sender's email address before clicking on links or
> > attachments as they may not be safe.
> >
> >             ― 1 file link ―
> >
> >             Found quite a few have been given for a few at MSKCC … for
> > example.
> >
> >
> >
> >               *   SCR_017850<
> > https://urldefense.com/v3/__https://scicrunch.org/resources/Any/search
> > ?q=SCR_017850&l=SCR_017850__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65lI9
> > i3lotEEL46o4XMF7Xe17We2_0sMhA716G-kwD$
> > > - Molecular Cytogenetics Core Facility
> >               *   SCR_017691<
> > https://urldefense.com/v3/__https://scicrunch.org/resources/Any/search
> > ?q=SCR_017691&l=SCR_017691__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65lI9
> > i3lotEEL46o4XMF7Xe17We2_0sMhA7_em4O2l$
> > > - Antibody & Bioresource Core Facility
> >               *   SCR_017853<
> > https://urldefense.com/v3/__https://scicrunch.org/resources/Any/search
> > ?q=SCR_017853&l=SCR_017853__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65lI9
> > i3lotEEL46o4XMF7Xe17We2_0sMhA79oyOBYh$
> > > - Investigational Products Support Core Facility
> >
> >             From: Frances Weis-Garcia <email obscured>>
> >             Date: Tuesday, June 2, 2020 at 11:48 AM
> >             To: "Core Rigor and Reproducibility (CoRRe)" <
> > <email obscured>>
> >             Subject: We have an RRID number
> >
> >             We have an RRID number
> >             But cannot find other cores with RRID numbers (yet)
> >
> >             Only matters if we want to define the structure of the
> > core RRID
> >
> >             [A screenshot of a cell phone  Description automatically
> > generated]
> >
> >             ― 1 file ―
> >
> >               □ image001.png  (386kb)
> >
> > https://urldefense.com/v3/__http://list.abrf.org/r/file/61rKfXKRRhmceb
> > jVxMqaDd73I7M-1CvB-2NFYRPN/__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65lI
> > 9i3lotEEL46o4XMF7Xe17We2_0sMhA7-cq3rnW$
> >
> >             ――
> >             View topic
> >
>
https://urldefense.com/v3/__http://list.abrf.org/r/topic/5kRaozb5cnXRgNPMB40Ica__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65lI9i3lotEEL46o4XMF7Xe17We2_0sMhA77Xsei7H$
> >             Leave group <email obscured>?Subject=Unsubscribe
> >
> >
> >
> >
> >         The information contained in this e-mail message may be
> > privileged, confidential, and/or protected from disclosure. This
> > e-mail message may contain protected health information (PHI);
> > dissemination of PHI should comply with applicable federal and state
> > laws. If you are not the intended recipient, or an authorized
> > representative of the intended recipient, any further review,
> > disclosure, use, dissemination, distribution, or copying of this
> > message or any attachment (or the information contained therein) is
> > strictly prohibited. If you think that you have received this e-mail
> > message in error, please notify the sender by return e-mail and delete
> > all references to it and its contents from your systems.
> >
> >
> >
> >
> > The information contained in this e-mail message may be privileged,
> > confidential, and/or protected from disclosure. This e-mail message
> > may contain protected health information (PHI); dissemination of PHI
> > should comply with applicable federal and state laws. If you are not
> > the intended recipient, or an authorized representative of the
> > intended recipient, any further review, disclosure, use,
> > dissemination, distribution, or copying of this message or any
> > attachment (or the information contained therein) is strictly
> > prohibited. If you think that you have received this e-mail message in
> > error, please notify the sender by return e-mail and delete all
> references to it and its contents from your systems.
> >
> > ――
> > View topic http://list.abrf.org/r/topic/5bRUHLyrqMfdch3xJ1egAo
> > Leave group <email obscured>?Subject=Unsubscribe
> >
> >
>
> ――
> View topic http://list.abrf.org/r/topic/7efFJlkEa5AdocqXi4oNC2
> Leave group <email obscured>?Subject=Unsubscribe
>
>
>
> The information in this e-mail is intended only for the person to whom it
> is
> addressed. If you believe this e-mail was sent to you in error and the
> e-mail
> contains patient information, please contact the Partners Compliance
> HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in
> error
> but does not contain patient information, please contact the sender and
> properly
> dispose of the e-mail.
>
> ――
> View topic http://list.abrf.org/r/topic/seIYKRgv7RlwME9A9WyC9
> Leave group <email obscured>?Subject=Unsubscribe
>
>

-----Original Message-----
From: Antibody Discussion Forum <email obscured>> On Behalf Of David
Blum
Sent: Wednesday, June 24, 2020 3:15 PM
To: <email obscured>
Subject: Re: [ABRF Ab Forum] FW: [EXT] Re: [core rigor and reproducibility
(corre)] We have an RRID number

Caution: External Sender


Laura,

Thanks for the note.  As a group, we haven't been active, but one thing we did
was the Virtual Lab Meeting (VLM).  I would be happy to setup a zoom
teleconference and anyone that would like to join could.  Perhaps the first
call could be for strategic planning, discussing what we would like to
accomplish then setup subsequent calls after that.  Previously the calls were
held at 11am EDT to accommodate people in Europe and on the West coast.

Would anyone be interested in me setting up a call next Monday June 29th at
11am EDT?

On Tue, Jun 23, 2020 at 10:12 PM Bover,Laura <email obscured>> wrote:

> Dear antibody guys,
> I hope all of you are doing fine.
> We are back in business since June 16th. Not sure for how long because
> Texas is raising in cases: today, Gov. Greg Abbott shared that Texas
> has seen 5,000 new coronavirus cases in a single day for the first
> time, the numbers are moving fast in the wrong direction.
> Our lab works, as probably yours, based on NCI funds and program income.
> Thus, as far as we can continue to work we are fine.
>
> On the other hand, this virus has made a breakthrough: everybody
> realize that immune system exists, and  everybody wants antibodies
> against everything. People that never work in antibodies titration
> before are titrating antibodies in plasma from recovered patients and
> publishing a Preprint with one of the main figures a graph of an
> ELISA!! Things that we usually only mention in Mat an Meth.
>
> With all that said I proposed Frances, if you would like to meet, once
> a month or once in a time, and see how are all you doing, how this is
> progressing and if you have contingencies plans in case you cannot
> work. Or simple share a good moment, or news or papers, being the case
> that one of the main treatments is so far convalescent plasma and
> hyperimmune polyclonal antibodies obtained and purified from horses,
> recombinant from llamas and eventually cloned from single B cells from
> recovered donors to use as poolsof antibodies.
>
> Approaches for vaccines developments. In fact all things that we are
> the experts! Immunizing to get immune response!
> May be write a review, talk , etc?
>
> Also I would like to know in a personal question is, how would my lab
> get a CLIA certification for making an ELISA for antibodies titration
> in this case, instead than a researcher that never worked with this,
> takes it as a potential result that may affect other's lives.
>
> What do you think?
> Can we do anything as a community of experts??
> Thanks!!
>
> Laura
> PS I would love to use zoom for these meetings and I am still thinking
> why the heck I didn't invest in Zoom's stocks!!
>
> Laura Bover, PhD
> Professor
> Director Monoclonal Antibody Core Facility University of Texas
> M.D.Anderson Cancer Center Associate member of The University of Texas
> Graduate School of Biomedical Sciences at Houston Member of the
> Scientific Review Committee 2 (former Clinical Research Committee 2)
> Member of the Steering Committee of the Immunology Program GSBS
> Immunology Department/ Genomics Medicine Department
> 7455 Fannin St Room 1SCR4.2021
> Houston- TX 77054
> T  713-563-3301 <tel:713-563-3301>
> F 713-563-3276 <tel:713-563-3276>
> <email obscured>
>
> In order to continue obtaining Core support in a challenging
> environment, it is crucial that our users acknowledge the following
> grants in all publications (see following text).  Thank you!
>  “We acknowledge the UT-MDACC Monoclonal Antibodies Core facility,
> supported in part by CCSG grant number P30 CA016672.”
>
>
>
>
>
> On 6/23/20, 2:11 PM, "Frances Weis-Garcia" <
> <email obscured>> wrote:
>
>     WARNING: This email originated from outside of MD Anderson. Please
> validate the sender's email address before clicking on links or
> attachments as they may not be safe.
>
>     I would love a monthly pow wow.
>     Please post to the list ... and let's see who is game.  It think
> you get a few takers.
>
>
>     On 6/23/20, 1:07 PM, "Bover,Laura" <email obscured>> wrote:
>
>         Hi Frances, I have just seen this email hidden under 200! I
> will read it and I will probably go ask with questions.
>         Should we organize a monthly meeting for MAbs group? Just to
> exchange situations in other Cores and states and cities and institutions?
>
>         One thing, I would like to know how a Core can have a
> certification CLIA is it is developed an assay for diagnostic. Let's
> say an ELISA, is that possible?
>         Thanks!
>         Laura
>
>         On 6/2/20, 10:52 AM, "Core Rigor and Reproducibility (CoRRe)
> on behalf of Frances Weis-Garcia" <email obscured> on behalf of
> <email obscured>> wrote:
>
>             WARNING: This email originated from outside of MD Anderson.
> Please validate the sender's email address before clicking on links or
> attachments as they may not be safe.
>
>             ― 1 file link ―
>
>             Found quite a few have been given for a few at MSKCC … for
> example.
>
>
>
>               *   SCR_017850<
> https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furld
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> > - Molecular Cytogenetics Core Facility
>               *   SCR_017691<
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> > - Antibody & Bioresource Core Facility
>               *   SCR_017853<
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> > - Investigational Products Support Core Facility
>
>             From: Frances Weis-Garcia <email obscured>>
>             Date: Tuesday, June 2, 2020 at 11:48 AM
>             To: "Core Rigor and Reproducibility (CoRRe)" <
> <email obscured>>
>             Subject: We have an RRID number
>
>             We have an RRID number
>             But cannot find other cores with RRID numbers (yet)
>
>             Only matters if we want to define the structure of the
> core RRID
>
>             [A screenshot of a cell phone  Description automatically
> generated]
>
>             ― 1 file ―
>
>               □ image001.png  (386kb)
>
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>             ――
>             View topic
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________________________________

Email Disclaimer: www.stjude.org/emaildisclaimer
Consultation Disclaimer: www.stjude.org/consultationdisclaimer

On 6/23/20, 2:11 PM, "Frances Weis-Garcia" <email obscured>>
wrote:

    WARNING: This email originated from outside of MD Anderson. Please validate
the sender's email address before clicking on links or attachments as they may
not be safe.

    I would love a monthly pow wow.
    Please post to the list ... and let's see who is game.  It think you get a
few takers.


    On 6/23/20, 1:07 PM, "Bover,Laura" <email obscured>> wrote:

        Hi Frances, I have just seen this email hidden under 200! I will read
it and I will probably go ask with questions.
        Should we organize a monthly meeting for MAbs group? Just to exchange
situations in other Cores and states and cities and institutions?

        One thing, I would like to know how a Core can have a certification
CLIA is it is developed an assay for diagnostic. Let's say an ELISA, is that
possible?
        Thanks!
        Laura

        On 6/2/20, 10:52 AM, "Core Rigor and Reproducibility (CoRRe) on behalf
of Frances Weis-Garcia" <email obscured> on behalf of
<email obscured>> wrote:

            WARNING: This email originated from outside of MD Anderson. Please
validate the sender's email address before clicking on links or attachments as
they may not be safe.

            ― 1 file link ―

            Found quite a few have been given for a few at MSKCC … for example.



              *
SCR_017850<https://urldefense.com/v3/__https://scicrunch.org/resources/Any/search?q=SCR_017850&l=SCR_017850__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65lI9i3lotEEL46o4XMF7Xe17We2_0sMhA716G-kwD$
> - Molecular Cytogenetics Core Facility
              *
SCR_017691<https://urldefense.com/v3/__https://scicrunch.org/resources/Any/search?q=SCR_017691&l=SCR_017691__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65lI9i3lotEEL46o4XMF7Xe17We2_0sMhA7_em4O2l$
> - Antibody & Bioresource Core Facility
              *
SCR_017853<https://urldefense.com/v3/__https://scicrunch.org/resources/Any/search?q=SCR_017853&l=SCR_017853__;!!PfbeBCCAmug!zp7khhc7NG1GfdpdSbJxcD65lI9i3lotEEL46o4XMF7Xe17We2_0sMhA79oyOBYh$
> - Investigational Products Support Core Facility

            From: Frances Weis-Garcia <email obscured>>
            Date: Tuesday, June 2, 2020 at 11:48 AM
            To: "Core Rigor and Reproducibility (CoRRe)" <email obscured>>
            Subject: We have an RRID number

            We have an RRID number
            But cannot find other cores with RRID numbers (yet)

            Only matters if we want to define the structure of the core RRID

            [A screenshot of a cell phone  Description automatically generated]

            ― 1 file ―

              □ image001.png  (386kb)
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        The information contained in this e-mail message may be privileged,
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The information contained in this e-mail message may be privileged,
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    On 6/1/20, 9:52 AM, "Antibody Discussion Forum on behalf of Frances
Weis-Garcia" <email obscured> on behalf of <email obscured>> wrote:

      WARNING: This email originated from outside of MD Anderson. Please
validate the sender's email address before clicking on links or attachments as
they may not be safe.

      Happy Monday.

      Is anyone aware of a technique to make ferret MAbs?

      Also, are you aware of hybridomas from any other species other than
mouse, rat, hamster, rabbits and humans?

      To the best of my knowledge, only mouse, rat and hamster hybriodmas are
generated with any efficiency and stability using mouse or rat myelomas.

      Frances
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