> On Oct 30, 2018, at 5:10 PM, Mark Sleeman
<email obscured>> wrote:
>
> Hi
> This was a timely post. We have also had lots of requests and are
> starting some work in this area. We will seek out those lines
> mentioned and keep people informed of progress. any additional
> information would be greatly appreciated
> Thanks
> Mark
>
>
>
> Sent from my iPhone
>
>> On 31 Oct 2018, at 06:15, David Blum <email obscured>> wrote:
>>
>> The 240E line is available from Katherine Knight at Loyola University via a
>> license. We have plans to use it, but have been told that it results in
>> unstable hybridomas. It is no longer available from the ATCC, BTW. Also,
>> I'm not a lawyer, but I believe we are restricted to our own use at our own
>> institution.
>>
>> https://en.wikipedia.org/wiki/Rabbit_hybridoma
>>
>>
>>
>> On Tue, Oct 30, 2018 at 3:51 PM Jason Goldstein <email obscured>>
>> wrote:
>>
>>>
>>> Does anyone have experience with development of rabbit monoclonals? I am
>>> unable to identify a myeloma line and unsure if still under license
>>> agreement. Can rabbit B-cells be fused by electrofusion similar to murine?
>>> Thanks,
>>> Jason
>>>
>>>
>>>
>>> Jason M. Goldstein
>>> Founder
>>>
>>> 7560 Portbury Park Lane
>>> Suwanee, GA 30024
>>>
>>> 415-519-4493
>>>
>>> <email obscured> | www.annularbio.com
>>>
>>>
>>>> On Oct 12, 2018, at 4:47 PM, Weis-Garcia, Frances/Sloan Kettering
>>> Institute <email obscured>> wrote:
>>>>
>>>> FYI - We use anti-mouse IgG - FITC (standard secondary) from Jackson
>>> ImmunoReagents ... and they definitely have an anti-human, so we are good.
>>>>
>>>> ο»Ώ-----Original Message-----
>>>> From: Antibody Discussion Forum <email obscured>> on behalf of
>>> Laura Bover <email obscured>>
>>>> Reply-To: <email obscured>>
>>>> Date: Friday, October 12, 2018 at 4:37 PM
>>>> To: <email obscured>" <email obscured>>
>>>> Cc: Lloyd Bourne <email obscured>>
>>>> Subject: Re: [ABRF Ab Forum] ClonePix
>>>>
>>>> Hi Philip,
>>>> We also have a Clonepix 2 but so far only used for hybridomas.
>>>> You will need the Detection fluorescence reagent from Molecular
>>> Devices that will help in the selection and pick up of the human IgG good
>>> producer clones. And I am not sure that they have a detection reagent for
>>> human IgGs. We use the murine detection reagent only.
>>>>
>>>> You may also try old fashion cloning by limited dilution. And test by
>>> ELISA and/or FACS the supernatants to select the best producers. We do a
>>> titration with supernatants and pick the bests.
>>>> I hope this helps.
>>>>
>>>> Thanks!
>>>>
>>>>
>>>> Laura Bover, PhD
>>>> Professor
>>>> Director Monoclonal Antibody Core Facility
>>>> University of Texas M.D.Anderson Cancer Center
>>>> Associate member of The University of Texas Graduate School of
>>> Biomedical Sciences at Houston
>>>> Immunology Department/ Genomics Medicine Department
>>>> 7455 Fannin St Room 1SCR4.3015/ 1901 East Rd- Room 4SCR5.1039
>>>> Houston- TX 77054
>>>> T 713-563-2281/ 713-563-3301
>>>> F 713-563-3276
>>>> <email obscured>
>>> <email obscured>
>>>>
>>>>
>>>>
>>>> On 10/12/18, 3:09 PM, "Antibody Discussion Forum on behalf of
>>> Weis-Garcia, Frances/Sloan Kettering Institute" <email obscured>
>>> on behalf of <email obscured>> wrote:
>>>>
>>>> Everyone in this discussion forum - Anyone have experience
>>> growing CHO cells in SS media?
>>>>
>>>> Philip, While you have them if you want to play around with
>>> culturing CHO cells in SS media that would save time. Molecular Devices
>>> and Stem Cell Technologies should have something ... maybe Fisher too. We
>>> have not, so we will need to run some pilots.
>>>>
>>>> Frances
>>>>
>>>> -----Original Message-----
>>>> From: Antibody Discussion Forum <email obscured>> on
>>> behalf of Phillip Gao <email obscured>>
>>>> Reply-To: <email obscured>>
>>>> Date: Friday, October 12, 2018 at 2:08 PM
>>>> To: <email obscured>" <email obscured>>
>>>> Cc: Lloyd Bourne <email obscured>>
>>>> Subject: Re: [ABRF Ab Forum] ClonePix
>>>>
>>>> Hi, Frances:
>>>>
>>>> Thank you so much!
>>>>
>>>> Yes, the antibody is humanized.
>>>>
>>>> I need the PI deliver the cells and medium to me, then make a
>>> bank. I will also take a close look of the manual and related materials so
>>> that know what I am doing before I send you everything. I will defiantly
>>> test the mycoplasma before I send them to you.
>>>>
>>>> Have a nice weekend.
>>>>
>>>> Philip
>>>>
>>>> -----Original Message-----
>>>> From: Antibody Discussion Forum <email obscured>> On
>>> Behalf Of Weis-Garcia, Frances/Sloan Kettering Institute
>>>> Sent: Friday, October 12, 2018 12:55 PM
>>>> To: <email obscured>
>>>> Cc: Lloyd Bourne <email obscured>>
>>>> Subject: Re: [ABRF Ab Forum] ClonePix
>>>>
>>>> We have one, and are open to trying.
>>>> We have only grown hybridomas but if the CHO are in
>>> suspension it should be fine.
>>>>
>>>> If you send the media (liquid and semisolid) and cells, we
>>> can give it try and only charge you if it works. Fair?
>>>>
>>>> Have the cells been tested for mycoplasma?
>>>> Please confirm the antibody has a human Fc domain.
>>>> If you do not tell me the antigen, I think we can avoid
>>> signing any MTA.
>>>>
>>>> Frances
>>>>
>>>> -----Original Message-----
>>>> From: Antibody Discussion Forum <email obscured>> on
>>> behalf of Phillip Gao <email obscured>>
>>>> Reply-To: <email obscured>>
>>>> Date: Friday, October 12, 2018 at 1:10 PM
>>>> To: <email obscured>" <email obscured>>
>>>> Subject: [ABRF Ab Forum] ClonePix
>>>>
>>>> Dear Friends:
>>>>
>>>> I have a pool of mixed antibody producing CHO suspension
>>> cells. The medium is commercially available. But the problem is the yield
>>> of the antibody (IgG) is very low, <100ug/l. We have the purified antigen.
>>>>
>>>> Does anyone have a ClonePix and how much would it cost
>>> for the service? Or any other better approaches?
>>>>
>>>> Rob at Vanderbilt had one and it is no longer in service.
>>>>
>>>> Thank you for your help.
>>>>
>>>> Philip
>>>>
>>>>
>>>> Philip Gao, Ph. D.
>>>> Director, COBRE Protein Production Group
>>>> Del Shankel Structural Biology Center
>>>> 2034 Becker Drive, Room 1095
>>>> University of Kansas
>>>> Lawrence, KS 66047
>>>> Phone: (785) 864-1825 (office); (785) 864-3406 (lab)
>>>> Fax: (785) 864-8141
>>>> Website: http://psf.cobre.ku.edu/cores/ppg/about
>>>>
>>>>
>>>>
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