The ABRF Discussion Forums will be retired on August 15 and this site will no longer be active. ABRFโs Core Community offers similar ways to share ideas and pose questions to ABRF members and specific interest groups. Please visit abrf.connectedcommunity.org to sign in to the Core Community. Thank you.
Nepal tourist visa can be extended. If you need to stay in Nepal for a few more days and the visa deadline is approaching, then you need to go to the Nepal National Immigration Bureau in Kathmandu to apply for a visa extension. If your visa has out of date less than 30 days, you need to pay for $3 fine per day. The delay can make you miss the flight, so you'd better deal with your visa well at Kathmandu's Central Immigration Office before the deadline. It's wise for tourists to prepare some passport photos in your bags. Because it could save much time when you are doing permits, visa applications, and other official documents. Travelers can extend the valid period of visa in Kathmandu and Pokhara and they only need to pay $30 for 15 days. Then, the $2 will be added per day after that. Extending 30 days will cost you $50, and the multiple-entry visa needs $20. Compared with the extension of the 60-day visa, you'd better hold the 90-day visa. To extend your visa, you should complete the requirement of the passport, the fee, one photo, and an application online. The photoshops charge for Rs 250 for eight digital passport photos. When you are in a line, you can prepare these documents well in advance. Normally, it takes travelers two hours to extend the visa, but you can pay Rs 300 for visa extensions for the purpose of saving time, and you will do it well within 10 minutes. Travel agencies also can help you deal with those things in several minutes. The maximum of the extension days is 150 within a year. Before the deadline for the visa, you must buy the air ticket and leave the country. Check the latest [url=https://www.greattibettour.com/permit/nepal-visa.htm]Nepal visa for US citizens[/url] information.
Our customers use a lot of peptides and the Company we trust more is Fisher scientific and in the past Sigma. WE used couple of times peptides from Genscript but we had several issues, such as the KLH conjugation to the peptide was lost or they did not know about the best method to conjugate KLH to cysteins. If you donโt have a cysteine in your peptide you can ask them to add one to the N or C terminal. And if you have cystein in the middle you have to ask them to block them because if not you will have more than one KLH in the peptide, which is not good because the immune response against the KLH will rise and probably not against the peptide. Please contact me if you have more questions. Laura Laura Bover, PhD Working from home during pandemic Cell 713-703-1951 Professor Director Monoclonal Antibody Core Facility University of Texas M.D.Anderson Cancer Center Associate member of The University of Texas Graduate School of Biomedical Sciences at Houston Member of the Scientific Review Committee 2 (former CRC 2) Member of the Steering Committee of the Immunology Program GSBS Immunology Department/ Genomics Medicine Department 7455 Fannin St Room 1SCR4.2021 Houston- TX 77054 T 713-563-3301 <tel:713-563-3301> F 713-563-3276 <tel:713-563-3276> <email obscured> In order to continue obtaining Core support in a challenging environment, it is crucial that our users acknowledge the following grants in all publications (see following text). Thank you! โThis work was supported in part by CCSG grant number P30 CA016672.โ iLab-Solutions links: https://mdanderson.ilabsolutions.com/service_center/show_external/3631 or: https://mdanderson.ilabsolutions.com/account/login If you do not already have an iLab account please create one prior to sample submission ๏ปฟOn 6/1/22, 7:00 AM, "Antibody Discussion Forum on behalf of Limourchik" <email obscured> on behalf of <email obscured>> wrote: WARNING: This email originated from outside of MD Anderson. Please validate the sender's email address before clicking on links or attachments as they may not be safe. When I was looking for some https://urldefense.com/v3/__https://paradigmpeptides.com/__;!!PfbeBCCAmug!leOAZlhaVSjUB_udX_uDnEIDr9XGTIQFRHpABTYYzC4moXsQlK83hoyU3TUx2VijibiMFfup7ORSIVF0H-d4l5E$ , I bumped into an article that described companies that offer custom peptide and peptide synthesis services. I've never needed something like this and never used the services of such companies. But I wouldn't recommend just getting the first company found on the internet. Better ask someone in the field for their recommendation or advice as I suppose you would need a qualitative product. And if you want some custom peptide synthesis services, you should be aware that additional testing will be necessary, so more investment is needed. If you can, share why you are looking for such a company and what product you intend to have. โโ View topic https://urldefense.com/v3/__http://list.abrf.org/r/topic/3Cg3R9pQWRPFnWVFEZ0PVe__;!!PfbeBCCAmug!leOAZlhaVSjUB_udX_uDnEIDr9XGTIQFRHpABTYYzC4moXsQlK83hoyU3TUx2VijibiMFfup7ORSIVF0jrrnsA4$ Leave group <email obscured>?Subject=Unsubscribe The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems.
When I was looking for some https://paradigmpeptides.com/, I bumped into an article that described companies that offer custom peptide and peptide synthesis services. I've never needed something like this and never used the services of such companies. But I wouldn't recommend just getting the first company found on the internet. Better ask someone in the field for their recommendation or advice as I suppose you would need a qualitative product. And if you want some custom peptide synthesis services, you should be aware that additional testing will be necessary, so more investment is needed. If you can, share why you are looking for such a company and what product you intend to have.
Sorry, this post is hidden. The reason that Limourchik gave for hiding the post is as follows:
sorry
If you think you should be able to see the post email support and we will see what we can do to help.
I have the same question like you[size=1][url=https://iogames-free.com]io games[/url][/size]
I'm tired of all my socks being monotonous and constantly tangled. Please help me buy wholesale custom socks.
Good afternoon. With such a problem faced by so many people in our time. To solve this problem I can advise you site https://www.customsocks.io/custom-socks-wholesale/ where you will sell wholesale custom socks. This site will help diversify your set of socks.
I'm tired of all my socks being monotonous and constantly tangled. Please help me buy wholesale custom socks.
I found this post very interesting and informative. Thank you for sharing your special thoughts with us. I definitely share this with my peeps.
A colleague of mine who develops therapeutic MAbs shared this with me ... hope this helps Scott: KabatMan; http://www.bioinf.org.uk/abs/simkab.html This is the old 'Kabat' database which has not been updated since 2000 Abysis; http://www.abysis.org/abysis/index.html Wonderful resource, one would use the 'Search' tools for what the posting asked Thera-SAbDab; http://opig.stats.ox.ac.uk/webapps/newsabdab/therasabdab/search/ Database of therapeutic antibodies which have been assigned an International Nonproprietary Name (INN; assignment typically occurs at some point in phase I or II) IMGT V-Quest; https://www.imgt.org/IMGT_vquest/input For germline assignment. Many other tools/databases on IMGT site. IgBlast; https://www.ncbi.nlm.nih.gov/igblast/ Also for germline assignment. References IMGT database I think the above is most of them. You can search against a sequence in each of the above, but if the desire is to download a database, options may be more limited. -----Original Message----- From: Antibody Discussion Forum <email obscured>> On Behalf Of Brown, Scott A Sent: Wednesday, March 9, 2022 10:58 AM To: <email obscured> Subject: [EXTERNAL] [ABRF Ab Forum] database? Good morning all, Is there a database for antibody sequences (mouse, human, etc) that can be searched, aligned, etc? It seems that Pubmed is limited and not very easy to do. I would love to compile several CDR loops from various antibodies. Hope everyone is having a great 2022! Best, Scott Scott Brown, Ph.D. Director of Immunologic Reagent Facility Department of Immunology St. Jude Children's Res. Hosp. w. (901) 595-4067 c. (901) 674-9960 ________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer Consultation Disclaimer: www.stjude.org/consultationdisclaimer โโ View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__list.abrf.org_r_topic_59JIuchtonZdfX4UBwNu18&d=DwIGaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=fmti5zNTSjO-18_DyVneGqexRrIKGyQy4zMQKtoqmRE&m=dK688vVgYw-rx5VArpaiVryZWTfIVqN9T8x3rZOapMHx41pAQgWbiVzIjdEwfCdO&s=uLVEJcmTxZng2y92Wr0Gt7mRkRtJesphJgBO0UiFGZQ&e= Leave group <email obscured>?Subject=Unsubscribe *** Only open attachments or links from trusted senders. Report phishing to <email obscured> *** ===================================================================== Please note that this e-mail and any files transmitted from Memorial Sloan Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer.
=====================================================================
Please note that this e-mail and any files transmitted from
Memorial Sloan Kettering Cancer Center may be privileged, confidential,
and protected from disclosure under applicable law. If the reader of
this message is not the intended recipient, or an employee or agent
responsible for delivering this message to the intended recipient,
you are hereby notified that any reading, dissemination, distribution,
copying, or other use of this communication or any of its attachments
is strictly prohibited. If you have received this communication in
error, please notify the sender immediately by replying to this message
and deleting this message, any attachments, and all copies and backups
from your computer.
I assume that you used the Kabat. Is this the one from PubMed? I used it from the real book 40 years ago! Laura Bover, PhD (working from home during COVID-19 Cell 713-703-1951) Professor Director Monoclonal Antibody Facility University of Texas M.D.Anderson Cancer Center Associate member of The University of Texas Graduate School of Biomedical Sciences at Houston Member of the Scientific Review Committee 2 Member of the Steering Committee of the Immunology Program GSBS Immunology Department/ Genomics Medicine Department 7455 Fannin St Room 1SCR4.2021 Houston- TX 77054 T 713-563-3301<tel:713-563-3301> F 713-563-3276<tel:713-563-3276> <email obscured><email obscured>> In order to continue obtaining Core support in a challenging environment, it is crucial that our users acknowledge the following grants in all publications (see following text). Thank you! โWe acknowledge the UT-MDACC Monoclonal Antibodies Core facility, supported in part by CCSG grant number P30 CA016672.โSent from my iPhone On Mar 9, 2022, at 9:58 AM, Brown, Scott A <email obscured>> wrote: ๏ปฟWARNING: This email originated from outside of MD Anderson. Please validate the sender's email address before clicking on links or attachments as they may not be safe. Good morning all, Is there a database for antibody sequences (mouse, human, etc) that can be searched, aligned, etc? It seems that Pubmed is limited and not very easy to do. I would love to compile several CDR loops from various antibodies. Hope everyone is having a great 2022! Best, Scott Scott Brown, Ph.D. Director of Immunologic Reagent Facility Department of Immunology St. Jude Children's Res. Hosp. w. (901) 595-4067 c. (901) 674-9960
________________________________ Email Disclaimer: https://urldefense.com/v3/__http://www.stjude.org/emaildisclaimer__;!!PfbeBCCAmug!ydZBmpSgu6wG1zH2Gz9t-le1R1c0VT_3_kIsU_TvC9qQ8v53uns1Rvy-nCi-4UWK$ Consultation Disclaimer: https://urldefense.com/v3/__http://www.stjude.org/consultationdisclaimer__;!!PfbeBCCAmug!ydZBmpSgu6wG1zH2Gz9t-le1R1c0VT_3_kIsU_TvC9qQ8v53uns1Rvy-nAy6cdf6$ โโ View topic https://urldefense.com/v3/__http://list.abrf.org/r/topic/59JIuchtonZdfX4UBwNu18__;!!PfbeBCCAmug!ydZBmpSgu6wG1zH2Gz9t-le1R1c0VT_3_kIsU_TvC9qQ8v53uns1Rvy-nP9FIe65$ Leave group <email obscured>?Subject=Unsubscribe The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems.
Good morning all, Is there a database for antibody sequences (mouse, human, etc) that can be searched, aligned, etc? It seems that Pubmed is limited and not very easy to do. I would love to compile several CDR loops from various antibodies. Hope everyone is having a great 2022! Best, Scott Scott Brown, Ph.D. Director of Immunologic Reagent Facility Department of Immunology St. Jude Children's Res. Hosp. w. (901) 595-4067 c. (901) 674-9960
________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer Consultation Disclaimer: www.stjude.org/consultationdisclaimer
I know that it depends on specific scenarios or requirements. Currently, I was looking for initial guidelines. So thank you for sharing this source. In case of further help, I will get back to you with one discipline for my case.
Totally depends on your requirement. Please also provide more information as even within the one discipline of chemistry the field of standards is very wide indeed. Otherwise check the following source for the general starting point of selection of analytical reference standards. https://www.symteraanalytics.com/Blog/Choose-Proper-Analytical-Reference-Standards
What are the selection criteria of analytical reference standards?
Hi Frances, We do 1:1, and I have not noticed any issue with stability. Best,
Scott -----Original Message----- From: Antibody Discussion Forum <email obscured>> On Behalf Of Frances Weis-Garcia Sent: Monday, February 21, 2022 2:40 PM To: <email obscured> Subject: [ABRF Ab Forum] Electrofusion - Ratio of splenocytes to myelomas Caution: External Sender. Do not open unless you know the content is safe. To those of you who do electrofusions ... what ratio of splenocytes to myelomas do you use. We used 1:1 and at one point moved up to 1.25 myelomas for each splenocyte. I noticed the stability of the hybridomas is less than ideal and want to bring the ratio down, so I would like to use your success as a benchmark. Thanks for sharing. Frances โโ View topic https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flist.abrf.org%2Fr%2Ftopic%2F72jDeNb2xX9JCwIxOJlNE8&data=04%7C01%7Cscott.brown%40stjude.org%7Cafc12fef69a141ce3e4d08d9f57a4b96%7C22340fa892264871b677d3b3e377af72%7C0%7C0%7C637810728301244748%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=4WCumctU%2BZmSP9s91tYwy5HfpeheCgZLHoCxvsPc6ks%3D&reserved=0 Leave group <email obscured>?Subject=Unsubscribe ________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer Consultation Disclaimer: www.stjude.org/consultationdisclaimer
To those of you who do electrofusions ... what ratio of splenocytes to myelomas do you use. We used 1:1 and at one point moved up to 1.25 myelomas for each splenocyte. I noticed the stability of the hybridomas is less than ideal and want to bring the ratio down, so I would like to use your success as a benchmark. Thanks for sharing.
Frances
Thanks for sharing Sheenah. We run into the problem during the final IV boost before the fusion โฆ so we have Benadryl on hand to rescue them. Did the IV route successfully drive the antibody response and what drove you to chose this route / when did you do it? Frances Sent on the fly from my mobile device On Feb 18, 2022, at 12:07 PM, Mische, Sheenah <email obscured>> wrote: ๏ปฟYes, for Ag (and particularly peptides) with low immunogenicity. But you do risk anaphylactic shock. Sheenah > On Feb 18, 2022, at 11:31 AM, Frances Weis-Garcia <email obscured>> wrote: > > ๏ปฟ[EXTERNAL] > > Happy Friday everyone. > > Hope you are all well. > > Have you ever done a IV injection for a standard boost (not the acute boost before harvesting the spleen)? > > A PAb company has it as part of one of their immunizations protocols โฆ only for 2 boosts โฆ and they say it really helps the titers. We have a poor responding target in mice and were thinking of using this in addition to SQ this next round. > > The immunogen is human cells and the target is a protein on the cells expressed at endogenous levels. Much to my dismay the lab is only accessing the response by a protein ELISA, but will do flow after this boost. > > โโ > View topic https://urldefense.com/v3/__http://list.abrf.org/r/topic/35HWG06UCTXeN6Gd0H2N17__;!!MXfaZl3l!MxbAMOW2F61--aZ-zrACtjOvGh46UNvBFz8Mvt9UF6QacmKMMS7I4QJm_Meyfoa3YmLP34c$
> Leave group <email obscured>?Subject=Unsubscribe > ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= โโ View topic http://list.abrf.org/r/topic/1K3eQliiWj6cn5sb91CTox Leave group <email obscured>?Subject=Unsubscribe *** Only open attachments or links from trusted senders. Report phishing to <email obscured> *** ===================================================================== Please note that this e-mail and any files transmitted from Memorial Sloan Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer.