Posts in Antibody Discussion Forum

Laura try this

ABRF President is inviting you to a scheduled Zoom meeting.

Join Zoom Meeting
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Meeting ID: 858 0524 5201
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Frances

ZRC 1563
Office     646-888-2354
Mobile   516-967-0656


On 12/14/20, 10:17 AM, "Antibody Discussion Forum on behalf of Bover,Laura"
<email obscured> on behalf of <email obscured>> wrote:

    Hi David I registered already. How will we access the webinar?

    Laura Bover, PhD
    (working from home during COVID-19 Cell 713-703-1951<tel:713-703-1951>)
    Professor
    Director Monoclonal Antibody Core Facility
    University of Texas M.D.Anderson Cancer Center
    Associate member of The University of Texas Graduate School of Biomedical
Sciences at Houston
    Member of the Scientific Review Committee 2 (former Clinical Research
Committee 2)
    Member of the Steering Committee of the Immunology Program GSBS
    Immunology Department/ Genomics Medicine Department
    7455 Fannin St<x-apple-data-detectors://2/1> Room 1SCR4.2021
    Houston- TX 77054<x-apple-data-detectors://3/0>
    T  713-563-3301<tel:713-563-3301>
    F 713-563-3276<tel:713-563-3276>
    <email obscured><email obscured>>

    In order to continue obtaining Core support in a challenging environment,
it is crucial that our users acknowledge the following grants in all
publications (see following text).  Thank you!
     “We acknowledge the UT-MDACC Monoclonal Antibodies Core facility,
supported in part by CCSG grant number P30 CA016672.”

    Sent from my iPhone

    On Dec 14, 2020, at 8:16 AM, David Blum <email obscured>> wrote:

    WARNING: This email originated from outside of MD Anderson. Please validate
the sender's email address before clicking on links or attachments as they may
not be safe.

    There is still time to register for the webinar this morning at 11am.  We
    are also looking for speakers for next year.  Please contact me if you
    would like to speak or are willing to invite a speaker.

    Antibody Technology Interest Network Presentation:
    The Link between T Follicular Helper Responses and Neutralizing Antibody
    and Memory B Cell Responses to SARS-CoV-2
    Monday, December 14, 2020 11:00 a.m.–12:00 p.m. ET

    Register here
<https://urldefense.com/v3/__https://us02web.zoom.us/meeting/register/tZEld-yqqj4oGtVYWVL_exfgItEG-6GfKrMo__;!!PfbeBCCAmug!0FaRI1ju5IqkEEQ021tPxW6JLr-sG4E9PJpUG_QoBLLh9hy859fGJ22kdNdX2W2j$
>

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Hi David I registered already. How will we access the webinar?

Laura Bover, PhD
(working from home during COVID-19 Cell 713-703-1951<tel:713-703-1951>)
Professor
Director Monoclonal Antibody Core Facility
University of Texas M.D.Anderson Cancer Center
Associate member of The University of Texas Graduate School of Biomedical
Sciences at Houston
Member of the Scientific Review Committee 2 (former Clinical Research Committee
2)
Member of the Steering Committee of the Immunology Program GSBS
Immunology Department/ Genomics Medicine Department
7455 Fannin St<x-apple-data-detectors://2/1> Room 1SCR4.2021
Houston- TX 77054<x-apple-data-detectors://3/0>
T  713-563-3301<tel:713-563-3301>
F 713-563-3276<tel:713-563-3276>
<email obscured><email obscured>>

In order to continue obtaining Core support in a challenging environment, it is
crucial that our users acknowledge the following grants in all publications
(see following text).  Thank you!
 “We acknowledge the UT-MDACC Monoclonal Antibodies Core facility, supported in
part by CCSG grant number P30 CA016672.”

Sent from my iPhone

On Dec 14, 2020, at 8:16 AM, David Blum <email obscured>> wrote:

WARNING: This email originated from outside of MD Anderson. Please validate
the sender's email address before clicking on links or attachments as they may
not be safe.

There is still time to register for the webinar this morning at 11am.  We
are also looking for speakers for next year.  Please contact me if you
would like to speak or are willing to invite a speaker.

Antibody Technology Interest Network Presentation:
The Link between T Follicular Helper Responses and Neutralizing Antibody
and Memory B Cell Responses to SARS-CoV-2
Monday, December 14, 2020 11:00 a.m.–12:00 p.m. ET

Register here
<https://urldefense.com/v3/__https://us02web.zoom.us/meeting/register/tZEld-yqqj4oGtVYWVL_exfgItEG-6GfKrMo__;!!PfbeBCCAmug!0FaRI1ju5IqkEEQ021tPxW6JLr-sG4E9PJpUG_QoBLLh9hy859fGJ22kdNdX2W2j$
>

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Dear All,

I have a non-profit customer that would like to have polyclonal antibodies made
in New Zealand white rabbits and purified. The antigen has been used
successfully in the past, and the polyclonal antibodies produced have been
marketed. Recently this customer has attempted to generate polyclonal
antibodies with a commercial entity. The attempt was unsuccessful. The customer
believes that they will have better results working with a university core
facility.

They would like to use the same animals and immunization routine that yielded
enantiomer specific antibodies. The polyclonal antibodies work with WB of cell
lysates and bind free hapten. The antigen is a hapten conjugated to KLH using
glutaraldehyde. The customer will provide the antigen.

Here is the immunization scheme:


     *   Freund's Complete Adjuvant was used for the primary immunization, and
boosts consisted of antigen alone.
        *   0.5 mL FCA + 500ug antigen in 0.5 mL
        *   0.25 mL injected into 4 sites SQ
My core does not work with rabbits. I am looking for an experienced group that
can move forward with this project quickly and be prepared to boost and collect
sera monthly from animals for 10 months using the protocol above. The screening
of the sera would be done by the customer prior to purification. The
purification would be done in your facility. In the past, this group used
ammonium sulphate precipitation methods. They are amenable to trying Protein
A/G columns.

Please let me know if you are interested in taking on this project.

Best regards,

Angel

Edith "Angel" Sampson, Ph.D.
Monoclonal Antibody Scientific Director

UF ICBR
2033 Mowry Road, Gainesville, FL 32610
Biotech.ufl.edu<https://www.biotech.ufl.edu/> | Tel: 352.273.8039| Fax:
352.273.8070

[cid:image001.png@01D6C88C.546DFFC0]

image001.png 9.3kb

SARS-CoV2 antibody responses: knowns and unknowns - - 11/16; 11:00 am ET

ABRF Antibody Technology Interest Network presentation

Join Professor Brianna Brewer, Drew University to discuss published data on
SARS-CoV2 antibody responses

Register here: https://lnkd.in/dXAP8Fz

*Antibody Technology Interest Network Presentation:*



*Rapid Discovery of Functional Antibodies for SARS-CoV-2*





The COVID-19 pandemic highlights the importance of a flexible and rapid
response to real-time development of medical countermeasures for
life-threatening viral pathogens. Using human B cells from survivors are a
potential source of therapeutic antibodies, Dr. Carnahan will describe the
pandemic response platform developed and deployed at the Vanderbilt Vaccine
Center that has led to a large panel of anti-viral antibodies directed at
SARS-CoV-2, including several antibodies currently in advanced clinical
development.

*Monday, October 26, 2020 11:00 a.m.–12:00 p.m. ET *Register here
<https://us02web.zoom.us/meeting/register/tZEscOiorTsvHNwYx2ZgZi_I9SH5l_OAD8Fj>

Register today to join us for this timely and informative presentation.



Speakers:



*Robert Carnahan, Ph.D.*
<https://www.vumc.org/crowe-lab/person/robert-carnahan-phd>
Associate Director, Vanderbilt Vaccine Center

Associate Professor, Pediatrics and Radiology and Radiologic Sciences

Vanderbilt University Medical Center



*David Blum, Ph.D.* <https://www.bmb.uga.edu/directory/people/david-blum>

Director, Bioexpression and Fermentation Facility

Director, Master of Biomanufacturing and Bioprocessing

University of Georgia

*[image: A screenshot of a cell phone Description automatically generated]*




*Monoclonal Antibodies from Bench to Bedside: Development in an Academic
Laboratory Core. Challenges*




*Monday, August 24, 2020 11:00 a.m.–12:00 p.m. ET **Add to Calendar
<https://us02web.zoom.us/meeting/tZYoduihqDwqHd3R3FpdHkIUCBBxdSejoq2R/ics>*

*Register Here
<https://us02web.zoom.us/j/82511967079?pwd=SUo5WG1rWG9aNGd4ZHUrQWQ0WDhTdz09>*

This presentation will address different approaches that successfully
develop an anti-immune checkpoint antibody and other antibodies licensed to
Pharma companies and in ongoing clinical trials. Other approaches under
development will also be addressed.



Register today to join us for this timely and informative presentation.



Speakers:



*David Blum, Ph.D.*

Director, Bioexpression and Fermentation Facility

Director, Master of Biomanufacturing and Bioprocessing

University of Georgia



*Laura Bover, PhD*

Professor

Depts. of Genomic Medicine/Immunology

Director, Monoclonal Antibodies Core Facility

MD Anderson Cancer Center
University of Texas

Yep
Once I hear from Ed.

Frances

ZRC 1563
Office     646-888-2354
Mobile   516-967-0656


On 7/30/20, 9:38 AM, "Antibody Discussion Forum on behalf of Bover,Laura"
<email obscured> on behalf of <email obscured>> wrote:

    Yes, please propose several times or later I send you my available ! Great!

    Laura Bover, PhD
    Professor
    Director Monoclonal Antibody Core Facility
    University of Texas M.D.Anderson Cancer Center
    Associate member of The University of Texas Graduate School of Biomedical
Sciences at Houston
    Member of the Scientific Review Committee 2 (former CRC 2)
    Member of the Steering Committee of the Immunology Program GSBS
    Immunology Department/ Genomics Medicine Department
    7455 Fannin St<x-apple-data-detectors://1/1> Room 1SCR4.2021
    Houston- TX 77054<x-apple-data-detectors://2/0>
    T  713-563-3301<tel:713-563-3301>
    F 713-563-3276<tel:713-563-3276>
    <email obscured><email obscured>>

    On Jul 30, 2020, at 7:53 AM, <email obscured>" <email obscured>>
wrote:

    WARNING: This email originated from outside of MD Anderson. Please validate
the sender's email address before clicking on links or attachments as they may
not be safe.

    Ed and Laura

        Can we set up a time learn about subtractive immunization early next
week?
        In the mean time I’ll or the cyclophosphamide.

    ARG Discussion Forum Members,

        Does anyone else want to attend?

    Frances


    Frances Weis-Garcia, Ph.D.

    Head
    Bi-Institutional Antibody and Bioresource Core Facility

    Associate Laboratory Member
    Immunology Program

    Memorial Sloan Kettering Cancer Center
    1275 York Avenue, New York, NY 10065
    T 646.888.2354   C 516.967.0656

    ABCF-MSKCC
website<https://urldefense.com/v3/__https://www.mskcc.org/research-advantage/core-facilities/monoclonal-antibody-core-facility__;!!PfbeBCCAmug!wVUFEDGZknG4iLngHzB3DVUQXfsU6doiBiI0jvvegef4wgmYdnvM0bvZQavGbEqZ$
>

    Adjunct Faculty
    Center for Clinical and Translational Science

    The Rockefeller University
    1230 York Avenue, New York, NY 10065
    T 212.327.7030

    ABCF-RU
website<https://urldefense.com/v3/__https://www.rockefeller.edu/monoclonal/__;!!PfbeBCCAmug!wVUFEDGZknG4iLngHzB3DVUQXfsU6doiBiI0jvvegef4wgmYdnvM0bvZQStlvFdx$
>


    Please note that this e-mail and any files transmitted with it may be
privileged, confidential, and protected from disclosure under applicable law.
If the reader of this message is not the intended recipient, or an employee or
agent responsible for delivering this message to the intended recipient, you
are hereby notified that any reading, dissemination, distribution, copying, or
other use of this communication or any of its attachments is strictly
prohibited.  If you have received this communication in error, please notify
the sender immediately by replying to this message and deleting this message,
any attachments, and all copies and backups from your computer.


    =====================================================================

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        Memorial Sloan Kettering Cancer Center may be privileged, confidential,
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Absolutely.

Frances

ZRC 1563
Office     646-888-2354
Mobile   516-967-0656


On 7/30/20, 10:18 AM, "Antibody Discussion Forum on behalf of Angel Sampson"
<email obscured> on behalf of <email obscured>> wrote:

    Dear all,

    Is it possible for me to join the Zoom call to learn more about subtractive
immunization. I have been wanting to try it here at UF.

    Kind regards,

    Angel

    Edith “Angel” Sampson, Ph.D.
    Monoclonal Antibody Scientific Director

    UF ICBR
    2033 Mowry Road, Gainesville, FL 32610
    Biotech.ufl.edu | Tel: 352.273.8039| Fax: 352.273.8070



    -----Original Message-----
    From: Antibody Discussion Forum <email obscured>> On Behalf Of
Bover,Laura
    Sent: Thursday, July 30, 2020 9:38 AM
    To: <email obscured>
    Subject: Re: [ABRF Ab Forum] [EXT] Subtractive Immunization

    [External Email]

    Yes, please propose several times or later I send you my available ! Great!

    Laura Bover, PhD
    Professor
    Director Monoclonal Antibody Core Facility University of Texas M.D.Anderson
Cancer Center Associate member of The University of Texas Graduate School of
Biomedical Sciences at Houston Member of the Scientific Review Committee 2
(former CRC 2) Member of the Steering Committee of the Immunology Program GSBS
Immunology Department/ Genomics Medicine Department
    7455 Fannin St<x-apple-data-detectors://1/1> Room 1SCR4.2021
    Houston- TX 77054<x-apple-data-detectors://2/0>
    T  713-563-3301<tel:713-563-3301>
    F 713-563-3276<tel:713-563-3276>
    <email obscured><email obscured>>

    On Jul 30, 2020, at 7:53 AM, <email obscured>" <email obscured>>
wrote:

    WARNING: This email originated from outside of MD Anderson. Please validate
the sender's email address before clicking on links or attachments as they may
not be safe.

    Ed and Laura

        Can we set up a time learn about subtractive immunization early next
week?
        In the mean time I’ll or the cyclophosphamide.

    ARG Discussion Forum Members,

        Does anyone else want to attend?

    Frances


    Frances Weis-Garcia, Ph.D.

    Head
    Bi-Institutional Antibody and Bioresource Core Facility

    Associate Laboratory Member
    Immunology Program

    Memorial Sloan Kettering Cancer Center
    1275 York Avenue, New York, NY 10065
    T 646.888.2354   C 516.967.0656

    ABCF-MSKCC
website<https://urldefense.com/v3/__https://www.mskcc.org/research-advantage/core-facilities/monoclonal-antibody-core-facility__;!!PfbeBCCAmug!wVUFEDGZknG4iLngHzB3DVUQXfsU6doiBiI0jvvegef4wgmYdnvM0bvZQavGbEqZ$
>

    Adjunct Faculty
    Center for Clinical and Translational Science

    The Rockefeller University
    1230 York Avenue, New York, NY 10065
    T 212.327.7030

    ABCF-RU
website<https://urldefense.com/v3/__https://www.rockefeller.edu/monoclonal/__;!!PfbeBCCAmug!wVUFEDGZknG4iLngHzB3DVUQXfsU6doiBiI0jvvegef4wgmYdnvM0bvZQStlvFdx$
>


    Please note that this e-mail and any files transmitted with it may be
privileged, confidential, and protected from disclosure under applicable law.
If the reader of this message is not the intended recipient, or an employee or
agent responsible for delivering this message to the intended recipient, you
are hereby notified that any reading, dissemination, distribution, copying, or
other use of this communication or any of its attachments is strictly
prohibited.  If you have received this communication in error, please notify
the sender immediately by replying to this message and deleting this message,
any attachments, and all copies and backups from your computer.


    =====================================================================

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Yes, please propose several times or later I send you my available ! Great!

Laura Bover, PhD
Professor
Director Monoclonal Antibody Core Facility
University of Texas M.D.Anderson Cancer Center
Associate member of The University of Texas Graduate School of Biomedical
Sciences at Houston
Member of the Scientific Review Committee 2 (former CRC 2)
Member of the Steering Committee of the Immunology Program GSBS
Immunology Department/ Genomics Medicine Department
7455 Fannin St<x-apple-data-detectors://1/1> Room 1SCR4.2021
Houston- TX 77054<x-apple-data-detectors://2/0>
T  713-563-3301<tel:713-563-3301>
F 713-563-3276<tel:713-563-3276>
<email obscured><email obscured>>

On Jul 30, 2020, at 7:53 AM, <email obscured>" <email obscured>> wrote:

WARNING: This email originated from outside of MD Anderson. Please validate
the sender's email address before clicking on links or attachments as they may
not be safe.

Ed and Laura

    Can we set up a time learn about subtractive immunization early next week?
    In the mean time I’ll or the cyclophosphamide.

ARG Discussion Forum Members,

    Does anyone else want to attend?

Frances


Frances Weis-Garcia, Ph.D.

Head
Bi-Institutional Antibody and Bioresource Core Facility

Associate Laboratory Member
Immunology Program

Memorial Sloan Kettering Cancer Center
1275 York Avenue, New York, NY 10065
T 646.888.2354   C 516.967.0656

ABCF-MSKCC
website<https://urldefense.com/v3/__https://www.mskcc.org/research-advantage/core-facilities/monoclonal-antibody-core-facility__;!!PfbeBCCAmug!wVUFEDGZknG4iLngHzB3DVUQXfsU6doiBiI0jvvegef4wgmYdnvM0bvZQavGbEqZ$
>

Adjunct Faculty
Center for Clinical and Translational Science

The Rockefeller University
1230 York Avenue, New York, NY 10065
T 212.327.7030

ABCF-RU
website<https://urldefense.com/v3/__https://www.rockefeller.edu/monoclonal/__;!!PfbeBCCAmug!wVUFEDGZknG4iLngHzB3DVUQXfsU6doiBiI0jvvegef4wgmYdnvM0bvZQStlvFdx$
>


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Ed and Laura

     Can we set up a time learn about subtractive immunization early next week?
     In the mean time I’ll or the cyclophosphamide.

ARG Discussion Forum Members,

     Does anyone else want to attend?

Frances


Frances Weis-Garcia, Ph.D.

Head
Bi-Institutional Antibody and Bioresource Core Facility

Associate Laboratory Member
Immunology Program

Memorial Sloan Kettering Cancer Center
1275 York Avenue, New York, NY 10065
T 646.888.2354   C 516.967.0656

ABCF-MSKCC
website<https://www.mskcc.org/research-advantage/core-facilities/monoclonal-antibody-core-facility>

Adjunct Faculty
Center for Clinical and Translational Science

The Rockefeller University
1230 York Avenue, New York, NY 10065
T 212.327.7030

ABCF-RU website<https://www.rockefeller.edu/monoclonal/>


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