Posts in Antibody Discussion Forum

I'm tired of all my socks being monotonous and constantly tangled. Please help
me buy wholesale custom socks.

I'm tired of all my socks being monotonous and constantly tangled. Please help
me buy wholesale custom socks.

A colleague of mine who develops therapeutic MAbs shared this with me ... hope
this helps Scott:

KabatMan; http://www.bioinf.org.uk/abs/simkab.html
This is the old 'Kabat' database which has not been updated since 2000

Abysis; http://www.abysis.org/abysis/index.html
Wonderful resource, one would use the 'Search' tools for what the posting asked

Thera-SAbDab; http://opig.stats.ox.ac.uk/webapps/newsabdab/therasabdab/search/
Database of therapeutic antibodies which have been assigned an International
Nonproprietary Name (INN; assignment typically occurs at some point in phase I
or II)

IMGT V-Quest; https://www.imgt.org/IMGT_vquest/input
For germline assignment. Many other tools/databases on IMGT site.

IgBlast; https://www.ncbi.nlm.nih.gov/igblast/
Also for germline assignment. References IMGT database

I think the above is most of them. You can search against a sequence in each of
the above, but if the desire is to download a database, options may be more
limited.

    -----Original Message-----
    From: Antibody Discussion Forum <email obscured>> On Behalf Of
Brown, Scott A
    Sent: Wednesday, March 9, 2022 10:58 AM
    To: <email obscured>
    Subject: [EXTERNAL] [ABRF Ab Forum] database?

    Good morning all,
    Is there a database for antibody sequences (mouse, human, etc) that can be
searched, aligned, etc?   It seems that Pubmed is limited and not very easy to
do.  I would love to compile several CDR loops from various antibodies.
    Hope everyone is having a great 2022!
    Best,
    Scott

    Scott Brown, Ph.D.
    Director of Immunologic Reagent Facility Department of Immunology St. Jude
Children's Res. Hosp.
    w. (901) 595-4067
    c. (901) 674-9960


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Good morning all,
Is there a database for antibody sequences (mouse, human, etc) that can be
searched, aligned, etc?   It seems that Pubmed is limited and not very easy to
do.  I would love to compile several CDR loops from various antibodies.
Hope everyone is having a great 2022!
Best,
Scott

Scott Brown, Ph.D.
Director of Immunologic Reagent Facility
Department of Immunology
St. Jude Children's Res. Hosp.
w. (901) 595-4067
c. (901) 674-9960

________________________________

Email Disclaimer: www.stjude.org/emaildisclaimer
Consultation Disclaimer: www.stjude.org/consultationdisclaimer

I know that it depends on specific scenarios or requirements. Currently, I was
looking for initial guidelines. So thank you for sharing this source. In case
of further help, I will get back to you with one discipline for my case.

What are the selection criteria of analytical reference standards?

To those of you who do electrofusions ... what ratio of splenocytes to myelomas
do you use.  We used 1:1 and at one point moved up to 1.25 myelomas for each
splenocyte.  I noticed the stability of the hybridomas is less than ideal and
want to bring the ratio down, so I would like to use your success as a
benchmark.

Thanks for sharing.

Frances

Yes, for Ag (and particularly peptides) with low immunogenicity.  But you do
risk anaphylactic shock.

Sheenah



> On Feb 18, 2022, at 11:31 AM, Frances Weis-Garcia
<email obscured>> wrote:
>
> [EXTERNAL]
>
> Happy Friday everyone.
>
> Hope you are all well.
>
> Have you ever done a IV injection for a standard boost (not the acute boost
before harvesting the spleen)?
>
> A PAb company has it as part of one of their immunizations protocols … only
for 2 boosts … and they say it really helps the titers.  We have a poor
responding target in mice and were thinking of using this in addition to SQ
this next round.
>
> The immunogen is human cells and the target is a protein on the cells
expressed at endogenous levels.  Much to my dismay the lab is only accessing
the response by a protein ELISA, but will do flow after this boost.
>
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Vivian, this is not a place to advertise but rather to share knowledge and hash
things our.  Should want to continue to participate, please do not post things
about your company's services or products.  Thank you.

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