Posts in Antibody Discussion Forum

Hi all,
I know about 5 years back we discussed doing a new study on antibody
sequencing.  Well, I'm in the process of starting to do that and I am reaching
out for guidance, primers, etc.  We will only be doing murine stuff right now,
but any advice would be appreciated!
All my best,
Scott

Scott Brown, Ph.D.
Director of Immunologic Reagent Facility
Department of Immunology
St. Jude Children's Res. Hosp.
w. (901) 595-4067
c. (901) 674-9960

________________________________

Email Disclaimer: www.stjude.org/emaildisclaimer
Consultation Disclaimer: www.stjude.org/consultationdisclaimer

This Director of Antibody Technology Core is a really sweet position for
someone who want to run more than a basic antibody core and would like to live
in Seattle. They have lots of high end equipment and a broad spectrum of
services.  More liike a small biotech than an academic core.

Position:
https://careers-fhcrc.icims.com/jobs/18715/staff-scientist--director-of-antibody-technology-core/job?mobile=false&width=1332&height=500&bga=true&needsRedirect=false&jan1offset=-480&jun1offset=-420

Core Website:
https://www.fredhutch.org/en/research/shared-resources/core-facilities/antibody-technology.html

For those of you who know Ben Hoffstrom, he ran built this core and has moved
onto something new.  I am sure he would be happy to give you the inside scoop.
I know his boss a little from his time at MSK.

Thanks Laura

Sent from my iPhone

> On Feb 10, 2021, at 4:50 PM, Bover,Laura <email obscured>> wrote:
>
> Hi Frances
> One of my users used Genscript for humanization. The price was reasonable 2-3
years ago and the service quite good.
> I used JN Bio when we humanized five of our antibodies, they were amazing.
But I think that recently they are not longer offering the service to the
public, unless if I put them in contact. Which is not practical, I guess. if
you are not from MD Anderson and making antibodies with us.
>
> Laura Bover, PhD
> (working from home during COVID-19 Cell 713-703-1951<tel:713-703-1951>)
> Professor
> Director Monoclonal Antibody Core Facility
> University of Texas M.D.Anderson Cancer Center
> Associate member of The University of Texas Graduate School of Biomedical
Sciences at Houston
> Member of the Scientific Review Committee 2 (former Clinical Research
Committee 2)
> Member of the Steering Committee of the Immunology Program GSBS
> Immunology Department/ Genomics Medicine Department
> 7455 Fannin St<x-apple-data-detectors://2/1> Room 1SCR4.2021
> Houston- TX 77054<x-apple-data-detectors://3/0>
> T  713-563-3301<tel:713-563-3301>
> F 713-563-3276<tel:713-563-3276>
> <email obscured><email obscured>>
>
> In order to continue obtaining Core support in a challenging environment, it
is crucial that our users acknowledge the following grants in all publications
(see following text).  Thank you!
> “We acknowledge the UT-MDACC Monoclonal Antibodies Core facility, supported
in part by CCSG grant number P30 CA016672.”
>
> Sent from my iPhone
>
> On Feb 10, 2021, at 3:16 PM, Frances Weis-Garcia
<email obscured>> wrote:
>
> WARNING: This email originated from outside of MD Anderson. Please validate
the sender's email address before clicking on links or attachments as they may
not be safe.
>
> Happy 2021 ... hope it is off to a great start for everyone.
>
> Question ... Are there any CROs that you recommend or would never use again
for antibody development or engineering (e.g. humanization)?
>
> I am asking for a colleagues.  I do not use them nor have I heard of any that
are particularly good or bad, so I thought I would toss it out to this
wonderful group.
>
> Frances
> ――
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Hi Frances
One of my users used Genscript for humanization. The price was reasonable 2-3
years ago and the service quite good.
I used JN Bio when we humanized five of our antibodies, they were amazing. But
I think that recently they are not longer offering the service to the public,
unless if I put them in contact. Which is not practical, I guess. if you are
not from MD Anderson and making antibodies with us.

Laura Bover, PhD
(working from home during COVID-19 Cell 713-703-1951<tel:713-703-1951>)
Professor
Director Monoclonal Antibody Core Facility
University of Texas M.D.Anderson Cancer Center
Associate member of The University of Texas Graduate School of Biomedical
Sciences at Houston
Member of the Scientific Review Committee 2 (former Clinical Research Committee
2)
Member of the Steering Committee of the Immunology Program GSBS
Immunology Department/ Genomics Medicine Department
7455 Fannin St<x-apple-data-detectors://2/1> Room 1SCR4.2021
Houston- TX 77054<x-apple-data-detectors://3/0>
T  713-563-3301<tel:713-563-3301>
F 713-563-3276<tel:713-563-3276>
<email obscured><email obscured>>

In order to continue obtaining Core support in a challenging environment, it is
crucial that our users acknowledge the following grants in all publications
(see following text).  Thank you!
 “We acknowledge the UT-MDACC Monoclonal Antibodies Core facility, supported in
part by CCSG grant number P30 CA016672.”

Sent from my iPhone

On Feb 10, 2021, at 3:16 PM, Frances Weis-Garcia <email obscured>>
wrote:

WARNING: This email originated from outside of MD Anderson. Please validate
the sender's email address before clicking on links or attachments as they may
not be safe.

Happy 2021 ... hope it is off to a great start for everyone.

Question ... Are there any CROs that you recommend or would never use again for
antibody development or engineering (e.g. humanization)?

I am asking for a colleagues.  I do not use them nor have I heard of any that
are particularly good or bad, so I thought I would toss it out to this
wonderful group.

Frances
――
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Thanks!!

On 12/14/20, 10:04 AM, "Antibody Discussion Forum on behalf of
<email obscured>" <email obscured> on behalf of <email obscured>>
wrote:

    WARNING: This email originated from outside of MD Anderson. Please validate
the sender's email address before clicking on links or attachments as they may
not be safe.

    Laura try this

    ABRF President is inviting you to a scheduled Zoom meeting.

    Join Zoom Meeting
https://urldefense.com/v3/__https://us02web.zoom.us/w/85805245201?tk=r_0_Cv4nSnq5uMtw5exaqoyTzZO5l9U5iOrrh-ECako.DQIAAAAT-mQjERZYN05uMEZmRFFHV2lsZ3RQdlZzcTNnAAAAAAAAAAAAAAAAAAAAAAAAAAAA&pwd=cElET3hYZHlzdVpaU2VJUGI2bm13dz09__;!!PfbeBCCAmug!yc4EA8nAxVBpAMiuItVOw4Kq79_fftZtmYXz0odKC1IcMDX_aTokQTGAb1e6iGF4$


    Meeting ID: 858 0524 5201
    Passcode: 033875
    One tap mobile
    +16465588656,,85805245201#,,,,,,0#,,033875# US (New York)
    +13126266799,,85805245201#,,,,,,0#,,033875# US (Chicago)

    Dial by your location
            +1 646 558 8656 US (New York)
            +1 312 626 6799 US (Chicago)
            +1 301 715 8592 US (Washington D.C)
            +1 669 900 9128 US (San Jose)
            +1 253 215 8782 US (Tacoma)
            +1 346 248 7799 US (Houston)
    Meeting ID: 858 0524 5201
    Passcode: 033875
    Find your local number:
https://urldefense.com/v3/__https://us02web.zoom.us/u/kyEnPMjKr__;!!PfbeBCCAmug!yc4EA8nAxVBpAMiuItVOw4Kq79_fftZtmYXz0odKC1IcMDX_aTokQTGAb3I-PsKa$

    Frances

    ZRC 1563
    Office     646-888-2354
    Mobile   516-967-0656


    On 12/14/20, 10:17 AM, "Antibody Discussion Forum on behalf of Bover,Laura"
<email obscured> on behalf of <email obscured>> wrote:

        Hi David I registered already. How will we access the webinar?

        Laura Bover, PhD
        (working from home during COVID-19 Cell 713-703-1951<tel:713-703-1951>)
        Professor
        Director Monoclonal Antibody Core Facility
        University of Texas M.D.Anderson Cancer Center
        Associate member of The University of Texas Graduate School of
Biomedical Sciences at Houston
        Member of the Scientific Review Committee 2 (former Clinical Research
Committee 2)
        Member of the Steering Committee of the Immunology Program GSBS
        Immunology Department/ Genomics Medicine Department
        7455 Fannin St<x-apple-data-detectors://2/1> Room 1SCR4.2021
        Houston- TX 77054<x-apple-data-detectors://3/0>
        T  713-563-3301<tel:713-563-3301>
        F 713-563-3276<tel:713-563-3276>
        <email obscured><email obscured>>

        In order to continue obtaining Core support in a challenging
environment, it is crucial that our users acknowledge the following grants in
all publications (see following text).  Thank you!
         “We acknowledge the UT-MDACC Monoclonal Antibodies Core facility,
supported in part by CCSG grant number P30 CA016672.”

        Sent from my iPhone

        On Dec 14, 2020, at 8:16 AM, David Blum <email obscured>> wrote:

        WARNING: This email originated from outside of MD Anderson. Please
validate the sender's email address before clicking on links or attachments as
they may not be safe.

        There is still time to register for the webinar this morning at 11am.
We
        are also looking for speakers for next year.  Please contact me if you
        would like to speak or are willing to invite a speaker.

        Antibody Technology Interest Network Presentation:
        The Link between T Follicular Helper Responses and Neutralizing
Antibody
        and Memory B Cell Responses to SARS-CoV-2
        Monday, December 14, 2020 11:00 a.m.–12:00 p.m. ET

        Register here
<https://urldefense.com/v3/__https://us02web.zoom.us/meeting/register/tZEld-yqqj4oGtVYWVL_exfgItEG-6GfKrMo__;!!PfbeBCCAmug!0FaRI1ju5IqkEEQ021tPxW6JLr-sG4E9PJpUG_QoBLLh9hy859fGJ22kdNdX2W2j$
>

        ――
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authorized representative of the intended recipient, any further review,
disclosure, use, dissemination, distribution, or copying of this message or any
attachment (or the information contained therein) is strictly prohibited. If
you think that you have received this e-mail message in error, please notify
the sender by return e-mail and delete all references to it and its contents
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         responsible for delivering this message to the intended recipient,
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The information contained in this e-mail message may be privileged,
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authorized representative of the intended recipient, any further review,
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attachment (or the information contained therein) is strictly prohibited. If
you think that you have received this e-mail message in error, please notify
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Laura,

You should receive  the info for the webinar after you register.

On Mon, Dec 14, 2020 at 10:17 AM Bover,Laura <email obscured>> wrote:

> Hi David I registered already. How will we access the webinar?
>
> Laura Bover, PhD
> (working from home during COVID-19 Cell 713-703-1951<tel:713-703-1951>)
> Professor
> Director Monoclonal Antibody Core Facility
> University of Texas M.D.Anderson Cancer Center
> Associate member of The University of Texas Graduate School of Biomedical
> Sciences at Houston
> Member of the Scientific Review Committee 2 (former Clinical Research
> Committee 2)
> Member of the Steering Committee of the Immunology Program GSBS
> Immunology Department/ Genomics Medicine Department
> 7455 Fannin St<x-apple-data-detectors://2/1> Room 1SCR4.2021
> Houston- TX 77054<x-apple-data-detectors://3/0>
> T  713-563-3301<tel:713-563-3301>
> F 713-563-3276<tel:713-563-3276>
> <email obscured><email obscured>>
>
> In order to continue obtaining Core support in a challenging environment,
> it is crucial that our users acknowledge the following grants in all
> publications (see following text).  Thank you!
>  “We acknowledge the UT-MDACC Monoclonal Antibodies Core facility,
> supported in part by CCSG grant number P30 CA016672.”
>
> Sent from my iPhone
>
> On Dec 14, 2020, at 8:16 AM, David Blum <email obscured>> wrote:
>
> WARNING: This email originated from outside of MD Anderson. Please
> validate the sender's email address before clicking on links or attachments
> as they may not be safe.
>
> There is still time to register for the webinar this morning at 11am.  We
> are also looking for speakers for next year.  Please contact me if you
> would like to speak or are willing to invite a speaker.
>
> Antibody Technology Interest Network Presentation:
> The Link between T Follicular Helper Responses and Neutralizing Antibody
> and Memory B Cell Responses to SARS-CoV-2
> Monday, December 14, 2020 11:00 a.m.–12:00 p.m. ET
>
> Register here
> <
>
https://urldefense.com/v3/__https://us02web.zoom.us/meeting/register/tZEld-yqqj4oGtVYWVL_exfgItEG-6GfKrMo__;!!PfbeBCCAmug!0FaRI1ju5IqkEEQ021tPxW6JLr-sG4E9PJpUG_QoBLLh9hy859fGJ22kdNdX2W2j$
> >
>
> ――
> View topic
>
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> Leave group <email obscured>?Subject=Unsubscribe
>
> The information contained in this e-mail message may be privileged,
> confidential, and/or protected from disclosure. This e-mail message may
> contain protected health information (PHI); dissemination of PHI should
> comply with applicable federal and state laws. If you are not the intended
> recipient, or an authorized representative of the intended recipient, any
> further review, disclosure, use, dissemination, distribution, or copying of
> this message or any attachment (or the information contained therein) is
> strictly prohibited. If you think that you have received this e-mail
> message in error, please notify the sender by return e-mail and delete all
> references to it and its contents from your systems.
>
> ――
> View topic http://list.abrf.org/r/topic/7v0YX5vmhZUVMBr2rCj3b9
> Leave group <email obscured>?Subject=Unsubscribe
>
>

There is still time to register for the webinar this morning at 11am.  We
are also looking for speakers for next year.  Please contact me if you
would like to speak or are willing to invite a speaker.

Antibody Technology Interest Network Presentation:
The Link between T Follicular Helper Responses and Neutralizing Antibody
and Memory B Cell Responses to SARS-CoV-2
Monday, December 14, 2020 11:00 a.m.–12:00 p.m. ET

Register here
<https://us02web.zoom.us/meeting/register/tZEld-yqqj4oGtVYWVL_exfgItEG-6GfKrMo>

This is a reminder to sign up for the webinar on Monday.  Dr Brianne Barker
is one of the co-hosts of the popular podcast "This week in Virology" as
well as "Immune" so this should be really interesting.

---------- Forwarded message ---------
From: ken.schoppmann <email obscured>>
Date: Mon, Nov 9, 2020 at 1:20 PM
Subject: [ABRF Ab Forum] November webinar - SARS-CoV2 antibody responses:
knowns and unknowns - - 11/16; 11:00 am ET
To: <email obscured>>


SARS-CoV2 antibody responses: knowns and unknowns - - 11/16; 11:00 am ET

ABRF Antibody Technology Interest Network presentation

Join Professor Brianna Brewer, Drew University to discuss published data on
SARS-CoV2 antibody responses

Register here: https://lnkd.in/dXAP8Fz
――
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There's still to register for Monday's Antibody Technology Interest Network
presentation on

*Rapid Discovery of Functional Antibodies for SARS-CoV-2*

We have openings for speakers for our new antibody webinars in September
and November (dates flexible, but generally on Mondays at 11am EDT).  Let
me know if you would like to give a talk or know somebody that could give a
talk for either of those months.

David