Post in EXT] Epitope Mapping

Dear Laura,
 One concerns about peptide based  epitope mapping issue, some antibodies have,
not linear but conformation structure. if we use Octet, it is very hard to get
target epitope, such as  CxCL or other extracellular targets.  We have to think
a different way.

Thank you

Rest of post

Bhupal
________________________________
From: Antibody Discussion Forum <email obscured>> on behalf of
Bover,Laura <email obscured>>
Sent: Tuesday, September 24, 2019 6:37 PM
To: <email obscured> <email obscured>>
Cc: Hawke,David H <email obscured>>; Bover,Laura <email obscured>>
Subject: Re: [ABRF Ab Forum] [EXT] Epitope Mapping

MDAnderson has a Core facility to which I derive my customers that need epitope
mapping.
They don’t do the traditional overlapping peptides, but MS.
I cc here the Director of the Core David Hawke.
We should be able to do it with OCTET and peptides but we didn’t set up the
protocol yet.
Hope this helps.
Thanks
Laura

Laura Bover, PhD
Professor
Director Monoclonal Antibody Core Facility
University of Texas M.D.Anderson Cancer Center
Associate member of The University of Texas Graduate School of Biomedical
Sciences at Houston
Immunology Department/ Genomics Medicine Department
7455 Fannin St Room 1SCR4.3015/ 1901 East Rd- Room 4SCR5.1039
Houston- TX 77054
T 713-563-2281/ 713-563-3301
F 713-563-3276
<email obscured>
<email obscured>>


On 9/24/19, 1:57 PM, "Thomas White" <email obscured>> wrote:

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    > On Sep 24, 2019, at 2:23 PM, Christensen, Kurt Richard <email obscured>>
wrote:
    >
    > ― 1 file link ―
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    > Hi All,
    > I have a customer that would like to have three mouse monoclonal
antibodies epitope mapped.  Does anyone here offer epitope mapping as a
service?   If so, please provide your contact information and I'll forward it
to them.  I would also be open to companies that offer this service if anyone
has a recommendation, although I would like to send the work to a shared
resource first.
    >
    > Thanks in advance!
    >
    >
    > Kurt
    >
    >
    >        Kurt Christensen
    >        Lab Director
    > Protein and Monoclonal Antibody Production Core
    >
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