Institute <email obscured>> wrote:
> Happy Friday.
>
> Do any of you produce and purify small amounts of the new MAbs you make
> for further characterization prior to committing to a few or one winner?
> If so, how do you do this. Currently we make 350 ml exhausted media
> (hybridoma adapted to 1% FBS prescreened for ultra-low levels of bovine IgG
> + 1 ng/ml IL6) and then purify it on a Prot G column using and FPLC. We
> get 1 β 8 mg, but I would like to scale this back and simply it if possible
> because we usually do not need that much.
>
> Thanks.
>
> Frances
>
>
> Frances Weis-Garcia, Ph.D.
>
> Head
> Bi-Institutional Antibody and Bioresource Core Facility
> (RRID: CR 017691)
>
> Associate Laboratory Member
> Immunology Program
>
> Memorial Sloan Kettering Cancer Center
> 1275 York Avenue, New York, NY 10065
>
<https://www.google.com/maps/search/1275+York+Avenue,+New+York,+NY+10065?entry=gmail&source=g>
> T 646.888.2354 C 516.967.0656
>
> ABCF-MSKCC website<
>
https://www.mskcc.org/research-advantage/core-facilities/monoclonal-antibody-core-facility
> >
>
> Adjunct Faculty
> Center for Clinical and Translational Science
>
> The Rockefeller University
> 1230 York Avenue, New York, NY 10065
>
<https://www.google.com/maps/search/1230+York+Avenue,+New+York,+NY+10065?entry=gmail&source=g>
> T 212.327.7030
>
> ABCF-RU website<https://www.rockefeller.edu/monoclonal/>
>
>
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> ββ
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