Hi Frances, We do 1:1, and I have not noticed any issue with stability. Best,
Scott -----Original Message----- From: Antibody Discussion Forum <email obscured>> On Behalf Of Frances Weis-Garcia Sent: Monday, February 21, 2022 2:40 PM To: <email obscured> Subject: [ABRF Ab Forum] Electrofusion - Ratio of splenocytes to myelomas Caution: External Sender. Do not open unless you know the content is safe. To those of you who do electrofusions ... what ratio of splenocytes to myelomas do you use. We used 1:1 and at one point moved up to 1.25 myelomas for each splenocyte. I noticed the stability of the hybridomas is less than ideal and want to bring the ratio down, so I would like to use your success as a benchmark. Thanks for sharing. Frances ―― View topic https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flist.abrf.org%2Fr%2Ftopic%2F72jDeNb2xX9JCwIxOJlNE8&data=04%7C01%7Cscott.brown%40stjude.org%7Cafc12fef69a141ce3e4d08d9f57a4b96%7C22340fa892264871b677d3b3e377af72%7C0%7C0%7C637810728301244748%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=4WCumctU%2BZmSP9s91tYwy5HfpeheCgZLHoCxvsPc6ks%3D&reserved=0 Leave group <email obscured>?Subject=Unsubscribe ________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer Consultation Disclaimer: www.stjude.org/consultationdisclaimer