Thanks for sharing Sheenah.
We run into the problem during the final IV boost before the fusion … so we
have Benadryl on hand to rescue them.
Did the IV route successfully drive the antibody response and what drove you to
chose this route / when did you do it?
Frances
Sent on the fly from my mobile device
On Feb 18, 2022, at 12:07 PM, Mische, Sheenah
<email obscured>> wrote:
Yes, for Ag (and particularly peptides) with low immunogenicity. But you do
risk anaphylactic shock.
Sheenah
> On Feb 18, 2022, at 11:31 AM, Frances Weis-Garcia
<email obscured>> wrote:
>
> [EXTERNAL]
>
> Happy Friday everyone.
>
> Hope you are all well.
>
> Have you ever done a IV injection for a standard boost (not the acute boost
before harvesting the spleen)?
>
> A PAb company has it as part of one of their immunizations protocols … only
for 2 boosts … and they say it really helps the titers. We have a poor
responding target in mice and were thinking of using this in addition to SQ
this next round.
>
> The immunogen is human cells and the target is a protein on the cells
expressed at endogenous levels. Much to my dismay the lab is only accessing
the response by a protein ELISA, but will do flow after this boost.
>
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