Here is the paper where the authors developed their own primers.
https://pubmed.ncbi.nlm.nih.gov/10648866/
On Mon, Apr 19, 2021 at 11:08 AM Brown, Scott A <
Rest of post
<email obscured>> wrote:
> Thanks David!
>
> Scott
>
> Scott Brown, Ph.D.
> Director of Immunologic Reagent Facility
> Department of Immunology
> St. Jude Children’s Research Hospital
> 262 Danny Thomas Place
> Memphis, TN 38105
> ________________________________
> From: Antibody Discussion Forum <email obscured>> on behalf of
> David Blum <email obscured>>
> Sent: Monday, April 19, 2021 10:01:09 AM
> To: <email obscured> <email obscured>>
> Subject: Re: [ABRF Ab Forum] mAb sequencing
>
> Caution: External Sender. Do not open unless you know the content is safe.
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>
> Scott,
>
> Thanks for the note. There is a kit from Novagen (Mouse Ig-Primer, cat #
> 69831-3) with murine primers. There are a couple of papers with primers
> that people have tried that I will try and dig up.
>
> David
>
> On Fri, Apr 16, 2021 at 11:46 AM Brown, Scott A <
> <email obscured>> wrote:
>
> > Hi all,
> > I know about 5 years back we discussed doing a new study on antibody
> > sequencing. Well, I'm in the process of starting to do that and I am
> > reaching out for guidance, primers, etc. We will only be doing murine
> > stuff right now, but any advice would be appreciated!
> > All my best,
> > Scott
> >
> > Scott Brown, Ph.D.
> > Director of Immunologic Reagent Facility
> > Department of Immunology
> > St. Jude Children's Res. Hosp.
> > w. (901) 595-4067
> > c. (901) 674-9960
> >
> >
> >
> > ________________________________
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