Does anyone know about the solubility characteristics of collagen. According
to information on the internet it is insoluble in water, and the solubility is
increased in aqueous acid. We would like to know if it dissolves in chaotropic
reagents such as 6M guanidine HCl or urea
Kym
Kym Francis Faull, Ph.D.
Director, Pasarow Mass Spectrometry Laboratory
Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
Professor Emeritus, Department of Psychiatry and Biobehavioral Sciences
David Geffen School of Medicine at UCLA
Telephone: (310) 206 7881 (office)
Telephone: (310) 206 7886 (laboratory)
Facsimile: (310) 206 2161
Electronic mail: <email obscured> <email obscured>>
Professor Steve VanDoren at the University of Missouri (Biochemistry) could
help you with this question. He has worked with collagen for many years.
Email: <email obscured>
Title: PROFESSOR OF BIOCHEMISTRY
Department: Agriculture Biochemistry
Phone: (573) 884-6405
Address: 117 Schweitzer Hall
City: Columbia
State: MO
Beverly DaGue
(Retired Proteomics Chemist)
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Does anyone know about the solubility characteristics of collagen. According
to information on the internet it is insoluble in water, and the solubility is
increased in aqueous acid. We would like to know if it dissolves in chaotropic
reagents such as 6M guanidine HCl or urea
Kym
Kym Francis Faull, Ph.D.
Director, Pasarow Mass Spectrometry Laboratory
Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
Professor Emeritus, Department of Psychiatry and Biobehavioral Sciences
David Geffen School of Medicine at UCLA
Telephone: (310) 206 7881 (office)
Telephone: (310) 206 7886 (laboratory)
Facsimile: (310) 206 2161
Electronic mail: <email obscured> <email obscured>>
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Collagens are cross-linked and unless the cross-links are broken will not
dissolve well. I recommend reviewing the following publications.
Eyre DR, Weis MA, Wu JJ. Advances in collagen cross-link analysis. Methods.
2008 May;45(1):65-74. doi: 10.1016/j.ymeth.2008.01.002. PMID: 18442706; PMCID:
PMC2398701. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2398701/
Robins SP, Bailey AJ. The chemistry of the collagen cross-links.
Characterization of the products of reduction of skin, tendon and bone with
sodium cyanoborohydride. Biochem J. 1977 May 1;163(2):339-46. doi:
10.1042/bj1630339. PMID: 17400; PMCID: PMC1164702.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1164702/
"Reduction of tissues with sodium cyanoborohydride at pH7.4 gave results
identical with those obtained by KBH4 treatment."
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-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of Kym Faull
Sent: Tuesday, August 4, 2020 11:07 AM
To: <email obscured>
Subject: [ABRF Discussion Forum] Collagen solubility
Does anyone know about the solubility characteristics of collagen. According
to information on the internet it is insoluble in water, and the solubility is
increased in aqueous acid. We would like to know if it dissolves in chaotropic
reagents such as 6M guanidine HCl or urea
Kym
Kym Francis Faull, Ph.D.
Director, Pasarow Mass Spectrometry Laboratory Jane and Terry Semel Institute
for Neuroscience and Human Behavior at UCLA Professor Emeritus, Department of
Psychiatry and Biobehavioral Sciences David Geffen School of Medicine at UCLA
Telephone: (310) 206 7881 (office)
Telephone: (310) 206 7886 (laboratory)
Facsimile: (310) 206 2161
Electronic mail: <email obscured> <email obscured>>
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View topic http://list.abrf.org/r/topic/3c9XAvS4uLixnXqi7sgPh3
Leave group <email obscured>?Subject=Unsubscribe
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