Can anyone cite some references for using trypsin for digestions inside a C-18 SPE cartridge/tip? Andy Alpert PolyLC Inc.
In-tip digestion with trypsin
Hi Andy Attached is an old method that describes trypsin digestion in a ZIPTIP. There is an error in the protocol as a few lines were transposed, They should be at the start of the protocol not in the middle. The method worked quite well and is probably applicable to most C18 tips. Cheers Keith • wash ZipTip with 30 μL of 60% MeOH, 5% formic acid • condition ZipTip with 30 μL of 5% MeOH, 5% formic acid • wash ZipTip with 25 μL of water
-----Original Message----- From: ABRF Discussion Forum <email obscured>> On Behalf Of <email obscured> Sent: Monday, November 30, 2020 8:05 AM To: <email obscured> Subject: [ABRF Discussion Forum] In-tip digestion with trypsin Can anyone cite some references for using trypsin for digestions inside a C-18 SPE cartridge/tip? Andy Alpert PolyLC Inc. ―― View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__list.abrf.org_r_topic_3xtElhDlhTF2XdOiUoCyFI&d=DwIFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=Sj7Qby4YdMTJxPRgeiwA5rdssX98XecxgG3Wu_SFYGk&m=git4XhWxzI4fHEf2PsPFstDjRGKKvArtsdwjyrYGeBA&s=7BRvvj6cyJ3hd37FBvEMS6mw8BIso4Yx4ImOZ--LPEE&e= Leave group <email obscured>?Subject=Unsubscribe Please be advised that this email may contain confidential information. If you are not the intended recipient, please notify us by email by replying to the sender and delete this message. The sender disclaims that the content of this email constitutes an offer to enter into, or the acceptance of, any agreement; provided that the foregoing does not invalidate the binding effect of any digital or other electronic reproduction of a manual signature that is included in any attachment.
Thanks, Keith! I'll adapt these instructions to our packed-bed TopTips for a customer's application.
Andy -----Original Message----- From: ABRF Discussion Forum <email obscured>> On Behalf Of Ashman, Keith Sent: Sunday, November 29, 2020 7:56 PM To: <email obscured> Subject: Re: [ABRF Discussion Forum] In-tip digestion with trypsin ― 1 file link ― Hi Andy Attached is an old method that describes trypsin digestion in a ZIPTIP. There is an error in the protocol as a few lines were transposed, They should be at the start of the protocol not in the middle. The method worked quite well and is probably applicable to most C18 tips. Cheers Keith • wash ZipTip with 30 μL of 60% MeOH, 5% formic acid • condition ZipTip with 30 μL of 5% MeOH, 5% formic acid • wash ZipTip with 25 μL of water -----Original Message----- From: ABRF Discussion Forum <email obscured>> On Behalf Of <email obscured> Sent: Monday, November 30, 2020 8:05 AM To: <email obscured> Subject: [ABRF Discussion Forum] In-tip digestion with trypsin Can anyone cite some references for using trypsin for digestions inside a C-18 SPE cartridge/tip? Andy Alpert PolyLC Inc. ―― View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__list.abrf.org_r_topic_3xtElhDlhTF2XdOiUoCyFI&d=DwIFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=Sj7Qby4YdMTJxPRgeiwA5rdssX98XecxgG3Wu_SFYGk&m=git4XhWxzI4fHEf2PsPFstDjRGKKvArtsdwjyrYGeBA&s=7BRvvj6cyJ3hd37FBvEMS6mw8BIso4Yx4ImOZ--LPEE&e= Leave group <email obscured>?Subject=Unsubscribe Please be advised that this email may contain confidential information. If you are not the intended recipient, please notify us by email by replying to the sender and delete this message. The sender disclaims that the content of this email constitutes an offer to enter into, or the acceptance of, any agreement; provided that the foregoing does not invalidate the binding effect of any digital or other electronic reproduction of a manual signature that is included in any attachment. ― 1 file ― □ Trypsin digest in a ziptip.pdf (143kb) http://list.abrf.org/r/file/8o4EsksWUfYbHcSVBPl7k75KKtw-BgH-2Pns0ZC/ ―― View topic http://list.abrf.org/r/topic/6lCQ07kf3LymVteuES6kMO Leave group <email obscured>?Subject=Unsubscribe