Dear Matt,
Thank you so much for your help!
Your observation is very helpful! It looks like that this reagent at least is
not that harmful if it is water soluble.
We plan to wash them away before our downstream sample preparation work,
hopefully it won't hurt our sample quality during LCMS analysis. We will have
to test it.
Thanks again!
Rest of post
Best,
Hua
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of matt sweeney
Sent: Monday, November 9, 2020 12:48 PM
To: <email obscured>
Subject: [External] [ABRF Discussion Forum] Re: [ABRF Discussion Forum]
Allprotect® Reagent-LCMS
Hi Hua
I do not know the answer.
I did look at MSDS sheet at
https://sds.qiagen.com/ehswww/QIAGENwww/result/report.jsp?P_LANGU=E&P_SYS=4&P_SSN=10010&P_REP=00000000000000000003&P_RES=11412
It has some interesting facts. A very specific sounding 134.17 MW is one. The
fact that the material has a fairly low flash point of 79C, boiling point of
178C, and density of 1.1 are all listed. It says it is a substance not a
mixture. It is yellow.
With that and a high res instrument measurement or two one could likely nail
the structure. I would guess it goes into water but is on the non polar side.
You might make homeopathic levels of dilution on up and scan low to see if you
can see it. I always like to know the chemistry of what I use. The structure
would help. Some proteolysis inhibitors actually covalently modify the protein
and so would impact associated peptides, for example.
Have fun. Good luck.
Matt from phone
> On Nov 9, 2020, at 9:09 AM, Ding, Hua <email obscured>> wrote:
> Allprotect® Reagent (from Qiagen)
――
View topic http://list.abrf.org/r/topic/1W6g75iB3SJyBdIrOa77gD
Leave group <email obscured>?Subject=Unsubscribe
** This email originated from an EXTERNAL sender to CHOP. Proceed with
caution when replying, opening attachments, or clicking links. Do not disclose
your CHOP credentials, employee information, or protected health information to
a potential hacker**.
Hua
Well, its not a LOT of help.
But I saw this too. Maybe its more helpful.
It indicates mass spec is ok it looks like.
From the vendor.
https://www.qiagen.com/us/products/discovery-and-translational-research/sample-collection-stabilization/tissue-ffpe/allprotect-tissue-reagent/?clear=true#orderinginformation
Matt from phone
> On Nov 9, 2020, at 12:19 PM, Ding, Hua <email obscured>> wrote:
>
> Dear Matt,
>
> Thank you so much for your help!
>
> Your observation is very helpful! It looks like that this reagent at least is
not that harmful if it is water soluble.
>
> We plan to wash them away before our downstream sample preparation work,
hopefully it won't hurt our sample quality during LCMS analysis. We will have
to test it.
Rest of post
>
> Thanks again!
>
> Best,
>
> Hua
>
>
>
> -----Original Message-----
> From: ABRF Discussion Forum <email obscured>> On Behalf Of matt sweeney
> Sent: Monday, November 9, 2020 12:48 PM
> To: <email obscured>
> Subject: [External] [ABRF Discussion Forum] Re: [ABRF Discussion Forum]
Allprotect® Reagent-LCMS
>
> Hi Hua
> I do not know the answer.
> I did look at MSDS sheet at
>
>
https://sds.qiagen.com/ehswww/QIAGENwww/result/report.jsp?P_LANGU=E&P_SYS=4&P_SSN=10010&P_REP=00000000000000000003&P_RES=11412
>
> It has some interesting facts. A very specific sounding 134.17 MW is one.
The fact that the material has a fairly low flash point of 79C, boiling point
of 178C, and density of 1.1 are all listed. It says it is a substance not a
mixture. It is yellow.
>
> With that and a high res instrument measurement or two one could likely nail
the structure. I would guess it goes into water but is on the non polar side.
You might make homeopathic levels of dilution on up and scan low to see if you
can see it. I always like to know the chemistry of what I use. The structure
would help. Some proteolysis inhibitors actually covalently modify the protein
and so would impact associated peptides, for example.
>
> Have fun. Good luck.
>
> Matt from phone
>
>> On Nov 9, 2020, at 9:09 AM, Ding, Hua <email obscured>> wrote:
>> Allprotect® Reagent (from Qiagen)
>
> ――
> View topic http://list.abrf.org/r/topic/1W6g75iB3SJyBdIrOa77gD
> Leave group <email obscured>?Subject=Unsubscribe
>
>
>
>
> ** This email originated from an EXTERNAL sender to CHOP. Proceed with
caution when replying, opening attachments, or clicking links. Do not disclose
your CHOP credentials, employee information, or protected health information to
a potential hacker**.
>
> ――
> View topic http://list.abrf.org/r/topic/7jGf0oIFOxxACHtt1WMzIz
> Leave group <email obscured>?Subject=Unsubscribe
>
Dear Matt,
We will have to give a try to test this Allprotect reagent with our current
workflow.
We will find out.....
Rest of post
Best,
Hua
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of matt sweeney
Sent: Tuesday, November 10, 2020 12:17 AM
To: <email obscured>
Subject: [ABRF Discussion Forum] Re: [ABRF Discussion Forum] RE: [External]
[ABRF Discussion Forum] Re: [ABRF Discussion Forum] Allprotect® Reagent-LCMS
Hua
Well, its not a LOT of help.
But I saw this too. Maybe its more helpful.
It indicates mass spec is ok it looks like.
>From the vendor.
https://www.qiagen.com/us/products/discovery-and-translational-research/sample-collection-stabilization/tissue-ffpe/allprotect-tissue-reagent/?clear=true#orderinginformation
Matt from phone
> On Nov 9, 2020, at 12:19 PM, Ding, Hua <email obscured>> wrote:
>
> Dear Matt,
>
> Thank you so much for your help!
>
> Your observation is very helpful! It looks like that this reagent at least is
not that harmful if it is water soluble.
>
> We plan to wash them away before our downstream sample preparation work,
hopefully it won't hurt our sample quality during LCMS analysis. We will have
to test it.
>
> Thanks again!
>
> Best,
>
> Hua
>
>
>
> -----Original Message-----
> From: ABRF Discussion Forum <email obscured>> On Behalf Of matt sweeney
> Sent: Monday, November 9, 2020 12:48 PM
> To: <email obscured>
> Subject: [External] [ABRF Discussion Forum] Re: [ABRF Discussion Forum]
Allprotect® Reagent-LCMS
>
> Hi Hua
> I do not know the answer.
> I did look at MSDS sheet at
>
>
https://sds.qiagen.com/ehswww/QIAGENwww/result/report.jsp?P_LANGU=E&P_SYS=4&P_SSN=10010&P_REP=00000000000000000003&P_RES=11412
>
> It has some interesting facts. A very specific sounding 134.17 MW is one.
The fact that the material has a fairly low flash point of 79C, boiling point
of 178C, and density of 1.1 are all listed. It says it is a substance not a
mixture. It is yellow.
>
> With that and a high res instrument measurement or two one could likely nail
the structure. I would guess it goes into water but is on the non polar side.
You might make homeopathic levels of dilution on up and scan low to see if you
can see it. I always like to know the chemistry of what I use. The structure
would help. Some proteolysis inhibitors actually covalently modify the protein
and so would impact associated peptides, for example.
>
> Have fun. Good luck.
>
> Matt from phone
>
>> On Nov 9, 2020, at 9:09 AM, Ding, Hua <email obscured>> wrote:
>> Allprotect® Reagent (from Qiagen)
>
> ――
> View topic http://list.abrf.org/r/topic/1W6g75iB3SJyBdIrOa77gD
> Leave group <email obscured>?Subject=Unsubscribe
>
>
>
>
> ** This email originated from an EXTERNAL sender to CHOP. Proceed with
caution when replying, opening attachments, or clicking links. Do not disclose
your CHOP credentials, employee information, or protected health information to
a potential hacker**.
>
> ――
> View topic http://list.abrf.org/r/topic/7jGf0oIFOxxACHtt1WMzIz
> Leave group <email obscured>?Subject=Unsubscribe
>
――
View topic http://list.abrf.org/r/topic/1Zql3G7dGiJsRlV4xJu2fc
Leave group <email obscured>?Subject=Unsubscribe