Hello, Deb!
You aren't the only one fielding helpful speculation about my retirement in
response to this posting. Responding to your response:
1) I am not retiring.
2) PolyLC is still in business.
3) PolyLC will remain a going concern even after I do retire someday.
For the record, these items are coming from a lab, not affiliated with PolyLC,
that was just going to heave them into a dumpster. I offered to make them
available instead as a service to the ABRF membership. Let me emphasize that
you either have to come here to pick them up or arrange for someone else whose
name isn't Alpert to do so; we are not going to ship them anywhere. Yes, some
of these items do prompt nostalgia (a strip-chart recorder?), but some are
perfectly usable and are in widespread use in labs now.
Andy Alpert
<email obscured>
Rest of post
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of Debra McMiilen
Sent: Monday, May 23, 2022 10:04 PM
To: <email obscured>
Subject: Re: [ABRF Discussion Forum] free lab equipment (used)
You must be retiring?
This list, such nostalgia!
----- Original Message -----
From: aalpert <email obscured>>
To: <email obscured>
Sent: Mon, 23 May 2022 15:50:36 -0400 (EDT)
Subject: [ABRF Discussion Forum] free lab equipment (used)
The following items are available free, on condition that you pick it/them up
at PolyLC (in Columbia, MD); we're not going to ship anything. As far as I can
see, they're all functional, but I can confirm that with any specific item upon
request. Contact me off list if you want anything <email obscured>). They
have to be picked up within about 1 week from today:
1) Abbott microcentrifuge (looks good!).
2) Hermle 96-well centrifuge, model 2-360K
3) Yellow solvent cabinet (Justrite; full size)
4) Ritter sterilizer (steam autoclave)
5) Beckman CPR centrifuge (has cooling unit)
6) SpeedVac solvent cold trap
7) SpeedVac SVC 200 centrifuge (many wells)
8) PE model 100 HPLC pump [NOTE: A single piston/head pump; handy for flushing
columns offline]
9) PE UV/Vis spectrophotometer model Lambda 1
10) TOSOH TSK-6080 autosampler
11) Skyline trade show booth case
12) Dionex metal-free HPLC gradient pump + pulsed amperometric detector
13) Dionex UV/Vis detector
14) Beckman model 100A HPLC pump (2 heads)
15) Shandon column packing pump (closed piston type)
16) LabConco Cascade freeze dryer 8
17) Kratos UV/Vis HPLC detector, model Spectroflow 773
18) LKB 2221 strip chart recorder with integrator
Andy Alpert
(410) 992-5400
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You must be retiring?
This list, such nostalgia!
Rest of post
----- Original Message -----
From: aalpert <email obscured>>
To: <email obscured>
Sent: Mon, 23 May 2022 15:50:36 -0400 (EDT)
Subject: [ABRF Discussion Forum] free lab equipment (used)
The following items are available free, on condition that you pick it/them up
at PolyLC (in Columbia, MD); we're not going to ship anything. As far as I can
see, they're all functional, but I can confirm that with any specific item upon
request. Contact me off list if you want anything <email obscured>). They
have to be picked up within about 1 week from today:
1) Abbott microcentrifuge (looks good!).
2) Hermle 96-well centrifuge, model 2-360K
3) Yellow solvent cabinet (Justrite; full size)
4) Ritter sterilizer (steam autoclave)
5) Beckman CPR centrifuge (has cooling unit)
6) SpeedVac solvent cold trap
7) SpeedVac SVC 200 centrifuge (many wells)
8) PE model 100 HPLC pump [NOTE: A single piston/head pump; handy for flushing
columns offline]
9) PE UV/Vis spectrophotometer model Lambda 1
10) TOSOH TSK-6080 autosampler
11) Skyline trade show booth case
12) Dionex metal-free HPLC gradient pump + pulsed amperometric detector
13) Dionex UV/Vis detector
14) Beckman model 100A HPLC pump (2 heads)
15) Shandon column packing pump (closed piston type)
16) LabConco Cascade freeze dryer 8
17) Kratos UV/Vis HPLC detector, model Spectroflow 773
18) LKB 2221 strip chart recorder with integrator
Andy Alpert
(410) 992-5400
――
View topic http://list.abrf.org/r/topic/54XRKKZHaHhty2RoA8wfAH
Leave group <email obscured>?Subject=Unsubscribe
The following items are available free, on condition that you pick it/them up
at PolyLC (in Columbia, MD); we're not going to ship anything. As far as I can
see, they're all functional, but I can confirm that with any specific item upon
request. Contact me off list if you want anything <email obscured>). They
have to be picked up within about 1 week from today:
1) Abbott microcentrifuge (looks good!).
2) Hermle 96-well centrifuge, model 2-360K
3) Yellow solvent cabinet (Justrite; full size)
4) Ritter sterilizer (steam autoclave)
5) Beckman CPR centrifuge (has cooling unit)
6) SpeedVac solvent cold trap
7) SpeedVac SVC 200 centrifuge (many wells)
8) PE model 100 HPLC pump [NOTE: A single piston/head pump; handy for flushing
columns offline]
9) PE UV/Vis spectrophotometer model Lambda 1
10) TOSOH TSK-6080 autosampler
11) Skyline trade show booth case
12) Dionex metal-free HPLC gradient pump + pulsed amperometric detector
13) Dionex UV/Vis detector
14) Beckman model 100A HPLC pump (2 heads)
15) Shandon column packing pump (closed piston type)
16) LabConco Cascade freeze dryer 8
17) Kratos UV/Vis HPLC detector, model Spectroflow 773
18) LKB 2221 strip chart recorder with integrator
Andy Alpert
(410) 992-5400
Does anyone know of a core (yours or someone else's) that offers an
in gel zymography service?
Good afternoon ABRF -
I'm looking to find out which Institutions have purchased a NovaSeq 6000 and
have them in a Core Facility? If you do, might you share your pricing and
turnaround time for samples?
THANK YOU!
Susanna
Susanna Perkins
Director, Research Cores & Operations
Research Core Administration
Tel: (508) 856-8255
www.umassmed.edu/research/cores/<https://www.umassmed.edu/research/cores/>
[cid:image001.png@01D869F4.C8D6B2C0]
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Greetings,
I and our specialists have not been able to download the PRIDE upload tool.
Does anyone know if this is just a traffic issue?
or
if there is any alternative route I can take for uploading full submissions
for SILAC and DM type quantitative experiments.
Sorry to bring this up but I can't spend any more time on this and I am just
hoping there is some silly thing I am not doing.
Any recent experiences would be especially helpful.
All the best,
Eric
D. Eric Anderson
Mass Spectrometry Facility, NIDDK
NIH
Building 8 Room B2A19, 9000 Rockville Pike, Bethesda MD, 20892 USA
(301)-496-7546
Guys,
I had a 1090…loved it…but the system would freeze often during a run if your
program contained a 0% A or B.
We always had to start at e.g. 0.1% B or higher…never start at 0 % B. This was
noted bug in the 1090 back then…you can run it manually at 0% (or 100%) but
not during a run. There may have been a fix for this later on by HP…something
about a different fuse being used on one of the PC boards…’ can’t quite recall
that…it was 30 years ago!
Perhaps this is why you are having a problem…I couldn’t see the whole email
thread.
Best,
Dick
> On May 5, 2022, at 5:49 PM, Kym Faull <email obscured>> wrote:
>
> What I don’t understand is this: What if anything should I do now? The
instrument is running fine. Is there something that can/should be done in
advance of the computer failing?
>
> Kym,
>
> Kym Francis Faull, Ph.D.
> Director, Pasarow Mass Spectrometry Laboratory
> Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
> Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
Sciences
Rest of post
> David Geffen School of Medicine at UCLA
> Telephone: (310) 206 7881 (office)
> Telephone: (310) 206 7886 (laboratory)
> Facsimile: (310) 206 2161
> Electronic mail: <email obscured> <email obscured>>
>
>
>
>
>
>
>
>
>> On May 4, 2022, at 6:24 PM, J K <email obscured>> wrote:
>>
>> I am totally going to do a self plug on a chapter I wrote to address this
>> issue of using virtualization of control computers to extend the life of
>> legacy instrumentation.
>>
>> https://pubmed.ncbi.nlm.nih.gov/28188538/
>>
>> We run a lot of our older equipment on newer computers that are running
>> virtual images of the original control computers.
>>
>> I have tested the methodology as far back as Windows 2000. I am not sure
>> if it will work for NT, but a search on the web suggests that other people
>> have had some success here. The one gotcha will be if the control computer
>> uses a special interface card to talk to the instrumentation.
>>
>>
>>
>>
>>
>> On Wed, May 4, 2022 at 5:36 PM Kym Faull <email obscured>> wrote:
>>
>>> We use an old HP series II 1090 for a lot of off-line work. The attached
>>> computer is equally old and runs on Windows NT! I have been advised that
>>> the old computer could go out any time, and it would be prudent to have a
>>> backup in place before the failure occurs. Does anyone have in their junk
>>> pile one of the computers that drive the HP 1090 HPLC’s? Alternatively,
>>> apart from EBAY, where might I find such a computer. As always, any and
>>> and all advice will be appreciated.
>>>
>>> Kym
>>>
>>>
>>> Kym Francis Faull, Ph.D.
>>> Director, Pasarow Mass Spectrometry Laboratory
>>> Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
>>> Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
>>> Sciences
>>> David Geffen School of Medicine at UCLA
>>> Telephone: (310) 206 7881 (office)
>>> Telephone: (310) 206 7886 (laboratory)
>>> Facsimile: (310) 206 2161
>>> Electronic mail: <email obscured> <email obscured>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>> ――
>>> View topic http://list.abrf.org/r/topic/3M6XBnMXp47pxOIngknfDS
>>> Leave group <email obscured>?Subject=Unsubscribe
>>>
>>>
>>
>> ――
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>>
>
>
> ――
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> Leave group <email obscured>?Subject=Unsubscribe
>
My opinion, there could be better.
Number one thing: Get a USB IDE (I assume) HD reader and image the drive
to a file (or another workflow similar....)
Then you can:
* Acquire $100 ebay computer per DA, try to write the image to a different
hard drive and see if it boots
* Try to virtualize based on that image
* Keep it in case the current HD fails so you can just write the data to a
new HD and keep using the same computer
Rest of post
On Thu, May 5, 2022 at 2:51 PM Kym Faull <email obscured>> wrote:
> What I don’t understand is this: What if anything should I do now? The
> instrument is running fine. Is there something that can/should be done in
> advance of the computer failing?
>
> Kym,
>
> Kym Francis Faull, Ph.D.
> Director, Pasarow Mass Spectrometry Laboratory
> Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
> Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
> Sciences
> David Geffen School of Medicine at UCLA
> Telephone: (310) 206 7881 (office)
> Telephone: (310) 206 7886 (laboratory)
> Facsimile: (310) 206 2161
> Electronic mail: <email obscured> <email obscured>>
>
>
>
>
>
>
>
>
> > On May 4, 2022, at 6:24 PM, J K <email obscured>> wrote:
> >
> > I am totally going to do a self plug on a chapter I wrote to address this
> > issue of using virtualization of control computers to extend the life of
> > legacy instrumentation.
> >
> > https://pubmed.ncbi.nlm.nih.gov/28188538/
> >
> > We run a lot of our older equipment on newer computers that are running
> > virtual images of the original control computers.
> >
> > I have tested the methodology as far back as Windows 2000. I am not sure
> > if it will work for NT, but a search on the web suggests that other
> people
> > have had some success here. The one gotcha will be if the control
> computer
> > uses a special interface card to talk to the instrumentation.
> >
> >
> >
> >
> >
> > On Wed, May 4, 2022 at 5:36 PM Kym Faull <email obscured>> wrote:
> >
> >> We use an old HP series II 1090 for a lot of off-line work. The
> attached
> >> computer is equally old and runs on Windows NT! I have been advised
> that
> >> the old computer could go out any time, and it would be prudent to have
> a
> >> backup in place before the failure occurs. Does anyone have in their
> junk
> >> pile one of the computers that drive the HP 1090 HPLC’s? Alternatively,
> >> apart from EBAY, where might I find such a computer. As always, any and
> >> and all advice will be appreciated.
> >>
> >> Kym
> >>
> >>
> >> Kym Francis Faull, Ph.D.
> >> Director, Pasarow Mass Spectrometry Laboratory
> >> Jane and Terry Semel Institute for Neuroscience and Human Behavior at
> UCLA
> >> Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
> >> Sciences
> >> David Geffen School of Medicine at UCLA
> >> Telephone: (310) 206 7881 (office)
> >> Telephone: (310) 206 7886 (laboratory)
> >> Facsimile: (310) 206 2161
> >> Electronic mail: <email obscured> <email obscured>>
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >> ――
> >> View topic http://list.abrf.org/r/topic/3M6XBnMXp47pxOIngknfDS
> >> Leave group <email obscured>?Subject=Unsubscribe
> >>
> >>
> >
> > ――
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>
>
> ――
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> Leave group <email obscured>?Subject=Unsubscribe
>
>
What I don’t understand is this: What if anything should I do now? The
instrument is running fine. Is there something that can/should be done in
advance of the computer failing?
Kym,
Kym Francis Faull, Ph.D.
Director, Pasarow Mass Spectrometry Laboratory
Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
Sciences
David Geffen School of Medicine at UCLA
Telephone: (310) 206 7881 (office)
Telephone: (310) 206 7886 (laboratory)
Facsimile: (310) 206 2161
Electronic mail: <email obscured> <email obscured>>
Rest of post
> On May 4, 2022, at 6:24 PM, J K <email obscured>> wrote:
>
> I am totally going to do a self plug on a chapter I wrote to address this
> issue of using virtualization of control computers to extend the life of
> legacy instrumentation.
>
> https://pubmed.ncbi.nlm.nih.gov/28188538/
>
> We run a lot of our older equipment on newer computers that are running
> virtual images of the original control computers.
>
> I have tested the methodology as far back as Windows 2000. I am not sure
> if it will work for NT, but a search on the web suggests that other people
> have had some success here. The one gotcha will be if the control computer
> uses a special interface card to talk to the instrumentation.
>
>
>
>
>
> On Wed, May 4, 2022 at 5:36 PM Kym Faull <email obscured>> wrote:
>
>> We use an old HP series II 1090 for a lot of off-line work. The attached
>> computer is equally old and runs on Windows NT! I have been advised that
>> the old computer could go out any time, and it would be prudent to have a
>> backup in place before the failure occurs. Does anyone have in their junk
>> pile one of the computers that drive the HP 1090 HPLC’s? Alternatively,
>> apart from EBAY, where might I find such a computer. As always, any and
>> and all advice will be appreciated.
>>
>> Kym
>>
>>
>> Kym Francis Faull, Ph.D.
>> Director, Pasarow Mass Spectrometry Laboratory
>> Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
>> Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
>> Sciences
>> David Geffen School of Medicine at UCLA
>> Telephone: (310) 206 7881 (office)
>> Telephone: (310) 206 7886 (laboratory)
>> Facsimile: (310) 206 2161
>> Electronic mail: <email obscured> <email obscured>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>> ――
>> View topic http://list.abrf.org/r/topic/3M6XBnMXp47pxOIngknfDS
>> Leave group <email obscured>?Subject=Unsubscribe
>>
>>
>
> ――
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> Leave group <email obscured>?Subject=Unsubscribe
>
I really like the virtualization idea a lot but the other option is to find an
older computer available or something made to match a legacy computer. There
was a $100 NT computer on E-bay this morning but it might not have the right
adapters for your instrument.
I have the same issue for Windows XP computers and I was able to find a new box
for very little money. Substituting SSD for HDD (if this works) should help
extend the operating life a lot.
NIXSYS seems to have options as well though more expensive.
My two cents!
Eric
Eric
D. Eric Anderson
Mass Spectrometry Facility, NIDDK
NIH
Building 8 Room B2A19, 9000 Rockville Pike, Bethesda MD, 20892 USA
(301)-496-7546
Rest of post
________________________________________
From: ABRF Discussion Forum <email obscured>> on behalf of Kym Faull
<email obscured>>
Sent: 04 May 2022 21:28
To: <email obscured>
Subject: [EXTERNAL] Re: [ABRF Discussion Forum] Help with an old HP Series II
1090
OK you gave me this advice previously I now remember. i will study your
advice and will probably get back to you
Kym Francis Faull, Ph.D.
Director, Pasarow Mass Spectrometry Laboratory
Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
Sciences
David Geffen School of Medicine at UCLA
Telephone: (310) 206 7881 (office)
Telephone: (310) 206 7886 (laboratory)
Facsimile: (310) 206 2161
Electronic mail: <email obscured> <email obscured>>
> On May 4, 2022, at 6:24 PM, J K <email obscured>> wrote:
>
> I am totally going to do a self plug on a chapter I wrote to address this
> issue of using virtualization of control computers to extend the life of
> legacy instrumentation.
>
> https://pubmed.ncbi.nlm.nih.gov/28188538/
>
> We run a lot of our older equipment on newer computers that are running
> virtual images of the original control computers.
>
> I have tested the methodology as far back as Windows 2000. I am not sure
> if it will work for NT, but a search on the web suggests that other people
> have had some success here. The one gotcha will be if the control computer
> uses a special interface card to talk to the instrumentation.
>
>
>
>
>
> On Wed, May 4, 2022 at 5:36 PM Kym Faull <email obscured>> wrote:
>
>> We use an old HP series II 1090 for a lot of off-line work. The attached
>> computer is equally old and runs on Windows NT! I have been advised that
>> the old computer could go out any time, and it would be prudent to have a
>> backup in place before the failure occurs. Does anyone have in their junk
>> pile one of the computers that drive the HP 1090 HPLC’s? Alternatively,
>> apart from EBAY, where might I find such a computer. As always, any and
>> and all advice will be appreciated.
>>
>> Kym
>>
>>
>> Kym Francis Faull, Ph.D.
>> Director, Pasarow Mass Spectrometry Laboratory
>> Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
>> Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
>> Sciences
>> David Geffen School of Medicine at UCLA
>> Telephone: (310) 206 7881 (office)
>> Telephone: (310) 206 7886 (laboratory)
>> Facsimile: (310) 206 2161
>> Electronic mail: <email obscured> <email obscured>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>> ――
>> View topic
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OK you gave me this advice previously I now remember. i will study your
advice and will probably get back to you
Kym Francis Faull, Ph.D.
Director, Pasarow Mass Spectrometry Laboratory
Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
Sciences
David Geffen School of Medicine at UCLA
Telephone: (310) 206 7881 (office)
Telephone: (310) 206 7886 (laboratory)
Facsimile: (310) 206 2161
Electronic mail: <email obscured> <email obscured>>
Rest of post
> On May 4, 2022, at 6:24 PM, J K <email obscured>> wrote:
>
> I am totally going to do a self plug on a chapter I wrote to address this
> issue of using virtualization of control computers to extend the life of
> legacy instrumentation.
>
> https://pubmed.ncbi.nlm.nih.gov/28188538/
>
> We run a lot of our older equipment on newer computers that are running
> virtual images of the original control computers.
>
> I have tested the methodology as far back as Windows 2000. I am not sure
> if it will work for NT, but a search on the web suggests that other people
> have had some success here. The one gotcha will be if the control computer
> uses a special interface card to talk to the instrumentation.
>
>
>
>
>
> On Wed, May 4, 2022 at 5:36 PM Kym Faull <email obscured>> wrote:
>
>> We use an old HP series II 1090 for a lot of off-line work. The attached
>> computer is equally old and runs on Windows NT! I have been advised that
>> the old computer could go out any time, and it would be prudent to have a
>> backup in place before the failure occurs. Does anyone have in their junk
>> pile one of the computers that drive the HP 1090 HPLC’s? Alternatively,
>> apart from EBAY, where might I find such a computer. As always, any and
>> and all advice will be appreciated.
>>
>> Kym
>>
>>
>> Kym Francis Faull, Ph.D.
>> Director, Pasarow Mass Spectrometry Laboratory
>> Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
>> Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
>> Sciences
>> David Geffen School of Medicine at UCLA
>> Telephone: (310) 206 7881 (office)
>> Telephone: (310) 206 7886 (laboratory)
>> Facsimile: (310) 206 2161
>> Electronic mail: <email obscured> <email obscured>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>> ――
>> View topic http://list.abrf.org/r/topic/3M6XBnMXp47pxOIngknfDS
>> Leave group <email obscured>?Subject=Unsubscribe
>>
>>
>
> ――
> View topic http://list.abrf.org/r/topic/3cOdvcPgVObEwboflHhq7E
> Leave group <email obscured>?Subject=Unsubscribe
>
I am totally going to do a self plug on a chapter I wrote to address this
issue of using virtualization of control computers to extend the life of
legacy instrumentation.
https://pubmed.ncbi.nlm.nih.gov/28188538/
We run a lot of our older equipment on newer computers that are running
virtual images of the original control computers.
I have tested the methodology as far back as Windows 2000. I am not sure
if it will work for NT, but a search on the web suggests that other people
have had some success here. The one gotcha will be if the control computer
uses a special interface card to talk to the instrumentation.
Rest of post
On Wed, May 4, 2022 at 5:36 PM Kym Faull <email obscured>> wrote:
> We use an old HP series II 1090 for a lot of off-line work. The attached
> computer is equally old and runs on Windows NT! I have been advised that
> the old computer could go out any time, and it would be prudent to have a
> backup in place before the failure occurs. Does anyone have in their junk
> pile one of the computers that drive the HP 1090 HPLC’s? Alternatively,
> apart from EBAY, where might I find such a computer. As always, any and
> and all advice will be appreciated.
>
> Kym
>
>
> Kym Francis Faull, Ph.D.
> Director, Pasarow Mass Spectrometry Laboratory
> Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
> Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
> Sciences
> David Geffen School of Medicine at UCLA
> Telephone: (310) 206 7881 (office)
> Telephone: (310) 206 7886 (laboratory)
> Facsimile: (310) 206 2161
> Electronic mail: <email obscured> <email obscured>>
>
>
>
>
>
>
>
>
>
> ――
> View topic http://list.abrf.org/r/topic/3M6XBnMXp47pxOIngknfDS
> Leave group <email obscured>?Subject=Unsubscribe
>
>
We use an old HP series II 1090 for a lot of off-line work. The attached
computer is equally old and runs on Windows NT! I have been advised that the
old computer could go out any time, and it would be prudent to have a backup in
place before the failure occurs. Does anyone have in their junk pile one of
the computers that drive the HP 1090 HPLC’s? Alternatively, apart from EBAY,
where might I find such a computer. As always, any and and all advice will be
appreciated.
Kym
Kym Francis Faull, Ph.D.
Director, Pasarow Mass Spectrometry Laboratory
Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
Professor Emeritus on Recall, Department of Psychiatry and Biobehavioral
Sciences
David Geffen School of Medicine at UCLA
Telephone: (310) 206 7881 (office)
Telephone: (310) 206 7886 (laboratory)
Facsimile: (310) 206 2161
Electronic mail: <email obscured> <email obscured>>
Good morning all!
I'm looking to gather info about iLABS - who is using it and feedback on
implementing it and how it's working (both from a central perspective and
individual Core staff perspectives). We are exploring it for all 45+ of our
facilities including basic research areas, clinical areas and our Supply
Center(s).
Any input would be great! THANK YOU!
Susanna
Susanna Perkins
Director, Research Cores & Operations
Research Core Administration
Tel: (508) 856-8255
www.umassmed.edu/research/cores/<https://www.umassmed.edu/research/cores/>
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What are your opinions of it?
What are its strengths?
Where is it most applicable?
Thanks very much.
Mark
On Tue, Jul 20, 2021 at 3:08 PM Martha Vestling <
Rest of post
<email obscured>> wrote:
> Check out M. Wilm’s Arcadiate. It is in the Mac App Store and free.
> MMV
>
> Martha M. Vestling, PhD
> Department of Chemistry
> University of Wisconsin-Madison
> Madison, WI 53706
> <email obscured>
>
> ――
> View topic http://list.abrf.org/r/topic/7e5Ak0GQNi9iwa0vG5BwYM
> Leave group <email obscured>?Subject=Unsubscribe
>
> --
Dr. Mark A. Wingerd, PhD
524 W Conestoga St
New Holland, PA 17557-1131
Mobile & Text: 301-502-4866
Email: <email obscured>
Fax: 240-514-4225
G-VoIP & Text: 760-364-6746
Checking With the ABRF Community: Do Many Of You Use The Web Professional
Website: “Academia.Edu” ???
https://www.academia.edu/ ???
I purchased a subscription, for $110 per year.
It had some nice tools to build a profile and find citations.
But in many ways, it doesn't seem very useful.
See: https://independent.academia.edu/MarkWingerd
Thank you very much for the feedback.
The next RDM4mic meeting is set for May 16-17.
Among other things, 2 working groups are planned on the topics of training and
FAQ. Please have a look at the linked agenda. If you would like to focus on
another topic, please contact me <email obscured>).
This meeting will take place in combination with the Kick-off meeting of the
i3d project, to which you are all warmly invited.
Meeting date: 16/17.05.2022
Location: online (link will be sent in an extra mail)
Agenda:
https://github.com/German-BioImaging/RDM4mic/blob/master/meetings/2022-05/2022-05_agenda.md
As an aside I see that Sigma / Merck sell an Anti-pan-Ubiquitin Remnant Motif
(K-ε-GG) antibody and show a western blot of what must be modified proteins.
https://www.sigmaaldrich.com/GB/en/product/sigma/sab5701397
How would this work if the Anti-pan-Ubiquitin Remnant Motif (K-ε-GG) requires
trypsin digestion to reveal it?
Dear ABRF Forum,
I was hoping to get some wisdom of approaching the detection of ubiquitin
modified proteins / peptides.
Ideally I'd like recommended approaches / products / antibodies to look at
modified proteins by westerns / IP and then move on to enrichment and MS
analysis.
I can see people use
1. IP of target proteins then blot with Ub antibodies such as P1D4 in a
candidate-based approach
2. The anti-diglycine remnant (K-ε-GG) antibody to enrich peptides and MS for a
systems level approach
I'd quite like to be able to do something between 1 and 2 i.e. IP Ub-modified
proteins and blot to check for enrichment before moving to the anti-diglycine
remnant (K-ε-GG) antibody enrichment.
Thanks in advance
Jon