Hello, I am having a problem I cannot solve regarding “jagged” chromatographic peak shape affecting primarily the most intense peaks of a run but the less intense peaks of the same run look nice and symmetrical. I’ve attached two PNG files demonstrating this. The “bad peak” NL=6e8 is about 10-fold more intense than the “good peak” NL=8e7. This is from a 4hr gradient run so the peaks are all around 0.8 to 2 min wide at baseline. I would be overjoyed if someone could pinpoint the problem. I’ve been fighting this for some time and have done many things without any real improvement. My system is and LTQ Orbitrap, HPLC is Thermo Accela 600 with flow splitting to a 150u ID x 15cm pulled tip packed at high pressure (~5000 PSI) in hexane with a 1.8u C18 stationary phase. Direct load samples, no trap. Solvents are the usual water, formic acid and acetonitrile of highest quality. I have used 3u C18 and also see this. This seems to point to chromatography as the source of the problem. However, I wonder if the LTQ Orbitrap could instead be at fault since it is affecting the more intense peaks and not the less intense peaks. If it were chromatography, should it not affect all peaks? The Orbitrap passes all routine calibrations easily and they are performed every three weeks. Thanks in advance for any comments, experiences and help you can send my way. Cheers, Brian <email obscured><email obscured><email obscured><email obscured>]
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Hi antibody experts! Please see Svenja's request below -- advice and assistance would be greatly appreciated. Please be sure to include Svenja <email obscured>) in your response. Many thanks, Allis
________________________________ From: Svenja-Maria Pahlke <email obscured>> Sent: Monday, April 2, 2018 5:10 PM To: Theresa McLaughlin Subject: Acetylation Dear Theresa, Our post doc (Mario Fidanza) requires an acetylated version of a specific ganglioside as well as an antibody against this acetylated ganglioside. Unfortunately they aren’t commercially available. Would you be able to assist us with this? Thank you in advance. Best regards, Svenja Pahlke
At ABRF 2018 Meeting in Myrtle Beach, Illumina will be hosting an ‘invitation only’ evening discussion on issues affecting Next Generation Sequencing cores. The survey (link below) is to identify potential participants and gather topics for discussion. Since seating is limited, survey participation does not guarantee an invitation. Please respond by April 6th. https://www.surveymonkey.com/r/IlluminaDinDin Please contact me with any questions.
Seth
Hi Yishai, Do you see any improvements in transmembrane domain containing protein identification rates using the S-Trap method?
Brian ________________________________ From: ABRF Discussion Forum <email obscured>> on behalf of Yishai Levin <email obscured>> Sent: Monday, April 2, 2018 4:08:02 AM To: <email obscured> Subject: Re: [ABRF Discussion Forum] detergent removal CAUTION: This message originated from a non UMB, UMSOM, FPI, or UMMS email system. Whether the sender is known or not known, hover over any links before clicking and use caution opening attachments. Hi Yan, We have tried it and use it exclusively for all our whole lysate samples. It works with SDS, NP-40, TritonX and others. The yield is so high that we reduced our starting material from 100ug that we used for FASP to 10-20ug. Good luck, Yishai > On 1 Mar 2018, at 6:21 PM, Yan Wang <email obscured>> wrote: > > That's interesting. I love how this forum always brings new products to > attention. Have you tried it? Looks like a product I'm working on can > really benefit from it. > > Yan > > On Thu, Mar 1, 2018 at 11:06 AM, Neveu, John M. <email obscured>> > wrote: > >> Chris, >> >> I would spike it to 5% SDS, ultra-sonicate (Covaris) to shear DNA, reduce >> and alkylate, then throw it onto an S-Trap (http://www.protifi.com/s-trap/) >> for removal of all detergents and a 1 hour digestion. Clean peptides eluted >> from the S-Trap, ready for analysis or desalt for PO4 enrichment. We use >> this workflow for anything from IP-HuES (balls of sticky slime) to >> Arabidopsis preps (smoothies) in low ug to several mg quantities with great >> success. >> >> John Neveu >> >> -----Original Message----- >> From: ABRF Discussion Forum <email obscured>] On Behalf Of >> Chris Turck >> Sent: Thursday, March 01, 2018 7:26 AM >> To: <email obscured> >> Subject: [ABRF Discussion Forum] detergent removal >> >> for membrane protein extraction we are using the following buffer for cell >> lysis: >> 25 mM HEPES, 1 mM DTT, 20 mM MgCl2, 0.4% NP-40. >> >> Question: How can we get rid of NP-40 for LC-MS analysis? >> Thanks! >> Chris >> >> >> ―― >> View topic http://list.abrf.org/r/topic/5Gd3hhN8KW2zxEDoe18073 >> Leave group <email obscured>?Subject=Unsubscribe >> >> >> ―― >> View topic http://list.abrf.org/r/topic/50CrqIBfrIB1A37XooR7FH >> Leave group <email obscured>?Subject=Unsubscribe >> >> > > > -- > > Yan Wang, Ph.D. > > Director, Proteomics Core Facility > > 2225 Bioscience Research Building > > University of Maryland > > 4066 Campus Drive > > College Park, MD 20742 > > > > (301)405-6764 > > > > Office and lab location: > > 0109/0111 Biology Psychology Building > > 4094 Campus Drive > > College Park, MD 20742 > > ―― > View topic http://list.abrf.org/r/topic/3RZhTKkyDdbLJmnYblAmFW > Leave group <email obscured>?Subject=Unsubscribe > ―― View topic http://list.abrf.org/r/topic/6lYn4PGiv8GPQ6zJ18hcp4 Leave group <email obscured>?Subject=Unsubscribe
Hi Yan, We have tried it and use it exclusively for all our whole lysate samples. It works with SDS, NP-40, TritonX and others. The yield is so high that we reduced our starting material from 100ug that we used for FASP to 10-20ug. Good luck,
Yishai > On 1 Mar 2018, at 6:21 PM, Yan Wang <email obscured>> wrote: > > That's interesting. I love how this forum always brings new products to > attention. Have you tried it? Looks like a product I'm working on can > really benefit from it. > > Yan > > On Thu, Mar 1, 2018 at 11:06 AM, Neveu, John M. <email obscured>> > wrote: > >> Chris, >> >> I would spike it to 5% SDS, ultra-sonicate (Covaris) to shear DNA, reduce >> and alkylate, then throw it onto an S-Trap (http://www.protifi.com/s-trap/) >> for removal of all detergents and a 1 hour digestion. Clean peptides eluted >> from the S-Trap, ready for analysis or desalt for PO4 enrichment. We use >> this workflow for anything from IP-HuES (balls of sticky slime) to >> Arabidopsis preps (smoothies) in low ug to several mg quantities with great >> success. >> >> John Neveu >> >> -----Original Message----- >> From: ABRF Discussion Forum <email obscured>] On Behalf Of >> Chris Turck >> Sent: Thursday, March 01, 2018 7:26 AM >> To: <email obscured> >> Subject: [ABRF Discussion Forum] detergent removal >> >> for membrane protein extraction we are using the following buffer for cell >> lysis: >> 25 mM HEPES, 1 mM DTT, 20 mM MgCl2, 0.4% NP-40. >> >> Question: How can we get rid of NP-40 for LC-MS analysis? >> Thanks! >> Chris >> >> >> ―― >> View topic http://list.abrf.org/r/topic/5Gd3hhN8KW2zxEDoe18073 >> Leave group <email obscured>?Subject=Unsubscribe >> >> >> ―― >> View topic http://list.abrf.org/r/topic/50CrqIBfrIB1A37XooR7FH >> Leave group <email obscured>?Subject=Unsubscribe >> >> > > > -- > > Yan Wang, Ph.D. > > Director, Proteomics Core Facility > > 2225 Bioscience Research Building > > University of Maryland > > 4066 Campus Drive > > College Park, MD 20742 > > > > (301)405-6764 > > > > Office and lab location: > > 0109/0111 Biology Psychology Building > > 4094 Campus Drive > > College Park, MD 20742 > > ―― > View topic http://list.abrf.org/r/topic/3RZhTKkyDdbLJmnYblAmFW > Leave group <email obscured>?Subject=Unsubscribe >
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Great. do you have time tomorrow?
Frances 516-967-0656 On Wed, Mar 28, 2018, 6:52 AM slevine <email obscured>> wrote: > Hi Frances, > Happy to chat as well. Done a bit of work in that space > -S > ―― > View topic http://list.abrf.org/r/topic/jtsgrd8z14oIi20HFKNWI > Leave group <email obscured>?Subject=Unsubscribe > >
Hi Frances, Happy to chat as well. Done a bit of work in that space -S
Hi, I will be attending ABRF 2018. We have been doing ChIP-Seq in our core and hopefully will be able to help you. See you there. Thanks
FYI - Our ABRF Award recipient for for Outstanding Contributions to Biomolecular Technologiesis Amos Bairoch for his leadership in this metadata revolution. Particularly for the development of community resources such as UniProtKB/Swiss-Prot knowledgebase, PROSITE, ENZYME, and neXtProt. He will be attending the ENTIRE meeting and looks forward to interacting with the ABRF membership. So if this area is of interest to you, please make him feel welcome.
Is anyone / do you know of anyone, who does some form of CHiP and is attending ABRF 2018 next month? I am looking for someone who can appreciates the immunoprecipitation step well enough to teach and troubleshoot it. If so, please email me at <email obscured>. Hope to see many of you at in Myrtle Beach. The program as well as the venue will be well worth the time.
Hello ABRF; I have been seeing intermittent hydrolysis yields for AAA due to incomplete hydrolysis. Does anyone have a suggestion for ensuring the Eldex hydrolysis Teflon caps for the hydrolysis vessels do not leak? I have purchased new caps from Eldex and built Teflon washers to apply additional pressure to the sealing surface. I have even tried replacing the phenolic threaded cap portion to provide more grip on the glass vessel as they wear from repeated use and exposure to 110C. The Eldex suggestion is to just not overtighten the caps as the Teflon liner gets deformed. Thanks in advance for any input or suggestions for alternate venting caps.
_Darryl
Hate to say it, but that's a really hard core way for handling these things. I once had a video store near me send me to collections over a 5 dollar late fee. Guess who never went there again and now they are out of business! And yup they totally went out of business because I never used them again ...not Netflix or anything 😉 I know you are not too worried about losing their business...considering they never paid you... Just chalk it off as the cost of doing business and don't work with them again if you don’t want to. It sucks I know...
Cheers Brett -----Original Message----- From: ABRF Discussion Forum <email obscured>> On Behalf Of Andor J Kiss Sent: Sunday, March 25, 2018 6:41 AM To: <email obscured> Subject: Re: [ABRF Discussion Forum] Re: Non-payment by outside PIs -> Any recourse? Thanks, Well I drafted a letter (that I would consider sending) to his Program Officer and Director of NIH and sent it to him. Wouldn't you know, I got an almost instant response imagine that... It's incredible dealing with some of these people. Guess I learned the hard, hard way - never lift a finger without a PO in hand... Thanks All... On Fri, 2018-03-23 at 19:43 +0000, Perkins, Susanna wrote: > We have our own 'blacklist' per se... if we are struggling with a > payment, our Central Office communicates to all our facilities that > so-and-so is in arrears and if s/he reaches out to you for work, send > them to the Central Office and don't take anything from them. It's > not often it happens, but the last time, the PI was within State and > drove over immediately when he discovered his name was sent to all the > facilities. He came with credit card in hand ;-) > > Susanna Perkins > Director, Research Cores & Operations > Research Core Administration, S2-106 > Tel: (508) 856-8255 > Fax: (508) 856-2303 > E-Mail: <email obscured><email obscured>> > > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential, proprietary > and privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please contact the sender immediately and destroy or permanently > delete all copies of the original message. > > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>] On Behalf Of > <email obscured><email obscured>> > Sent: Friday, March 23, 2018 12:56 PM > To: <email obscured><email obscured>> > Subject: Re: [ABRF Discussion Forum] Re: Non-payment by outside PIs > -> Any recourse? > > We have discussed a black list of institutions within our institution. > So, if we do not get paid for work done for Dr. Smith at the U of > Mars, no one can do work for the U of Mars until the bill is paid. > > We are also not allowed to do work until we have a PO. To be honest, > there are times I start, but, like Brett, I never give them what they > requested without a PO in hand … and not just a PO number, an official > PO in the form of a pdf. I know I hate to do this paperwork stuff and > carrots always work for me … so I give them carrots too ;) > > -----Original Message----- > From: ABRF Discussion Forum <email obscured><email obscured>>> on behalf of Brett > Phinney <email obscured><email obscured>>> > Reply-To: <email obscured><email obscured>>" <email obscured><email obscured>>> > Date: Friday, March 23, 2018 at 12:47 PM > To: <email obscured><email obscured>>" <email obscured><email obscured>>> > Subject: [EXTERNAL] Re: [ABRF Discussion Forum] Non-payment by outside > PIs -> Any recourse? > > Common problem unfortunately, at one point a few months back I was > owed over 100K from a 10-20 different people maybe? Most of the time > it's just an oversite or clerical error at the host institution, on > the rare occasion where it's malicious and they have no intention of > paying just ban them or their entire institution (if you want to be > hard core) from using you again...that's probably the only thing you > can do. > > No ABRF bad PI list as far as I know...Not sure I would encourage > that either... > > Another strategy we sometimes use is not giving the clients the > data until we have the PO or credit card number... We don't do this > frequently...but it does work. Especially when you say "hey the data > looks awesome...as soon as we get your payment info we will send it > right over" > > > Cheers > > Brett > > > > -----Original Message----- > From: ABRF Discussion Forum <email obscured><email obscured>>> On Behalf Of > Perera, Anoja > Sent: Friday, March 23, 2018 6:56 AM > To: <email obscured><email obscured>> > Subject: Re: [ABRF Discussion Forum] Non-payment by outside PIs > -> Any recourse? > > Can you contact the institute? > > -----Original Message----- > From: ABRF Discussion Forum <email obscured><email obscured>>> On Behalf Of > Andor J Kiss > Sent: Friday, March 23, 2018 8:51 AM > To: <email obscured><email obscured>> > Subject: [ABRF Discussion Forum] Non-payment by outside PIs -> Any > recourse? > > Hi, > > Just ran into a situation wherein an outside PI > (academic) keep delaying their PO. Long story short, it never came and > now there is no response from the PI. > > Is there any recourse in this situation? Or is this a > Institution to Institution issue at this point? > > Does ABRF maintain a "Blacklist" of bad PIs? > > Thanks, > -- > _________________________________________________________________ > ______ > _________________________________________________ > Andor J Kiss, PhD > Director - Center for Bioinformatics & Functional Genomics > 086 Pearson Hall - Miami University > 700 East High Street, Oxford > Ohio 45056 > USA > > eMAIL: <email obscured><email obscured>> > Telephone: +1 (513) 529-4280 > Fax: +1 (513) 529-2431 > Ring ID: andorjkiss > > URL (CBFG): https://urldefense.proofpoint.com/v2/url?u=http-3A__m > iamioh.edu_cas_academics_centers_cbfg_&d=DwIFaQ&c=WJBj9sUF1mbpVIAf3bi > u3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=qog7ACeR5C6W7dTFJB3TO854O > WtGwuMjU0F3BxMTYBA&e= > URL (CBFG Services): https://urldefense.proofpoint.com/v2/url?u=h > ttps-3A__www.scienceexchange.com_labs_center-2Dfor- > 2Dbi&d=DwIFaQ&c=WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y- > DPMs-8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=7FEURx1auu0zzCc- > QA2KQVqw337I_O4oe-2xClhLFlQ&e= > oinformatics-functional-genomics > URL (Research): https://urldefense.proofpoint.com/v2/url?u=http-3 > A__openwetware.org_wiki_User-3AAndor-5FJ- > 5FKiss&d=DwIFaQ&c=WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8 > Y-DPMs-8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=e0rEPFjKQMqsrUeOIVJs4eEV2 > EjDMrj_83cjdzVdx1U&e= > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__li > st.abrf.org_r_topic_23hIOlZmyS6vK2bSqFYVPx&d=DwIFaQ&c=WJBj9sUF1mbpVIA > f3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=03ssiwVT3jvZtmgEdkRUS6RLD > G9txjqx44GhW6vOOr8&e= > Leave group <email obscured>?Subject=Unsubscribe > > > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__li > st.abrf.org_r_topic_2BpJFyy1iInrFalIYMY0o6&d=DwIFaQ&c=WJBj9sUF1mbpVIA > f3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=H3OE9k7ojCR4XNXY8HKaoHKbe > HLb1e8UeY509tRT_Ck&e= > Leave group <email obscured>?Subject=Unsubscribe > > > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__li > st.abrf.org_r_topic_4k26g7DaHFivk7JuD08Td3&d=DwIFaQ&c=WJBj9sUF1mbpVIA > f3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=cn7nTiykosr5jWRyXqafiIw2n > nHVm6wmMZ4Zbyao57c&e= > Leave group <email obscured>?Subject=Unsubscribe > > > > > *** Only open attachments or links from trusted senders. Report > phishing to <email obscured><email obscured>> *** > > > > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__list.a > brf.org_r_topic_qSOeOyFTp6c90to8wQ5Q5&d=DwIFaQ&c=WJBj9sUF1mbpVIAf3biu > 3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=Q76xxpgRXGt24WEUwLP1aUceJ > nKlDTib0TdUCxCryp0&e= > Leave group <email obscured>?Subject=Unsubscribe > > > ―― > View topic http://list.abrf.org/r/topic/2IVQtA4dgPzB4OwaxEtx0z > Leave group <email obscured>?Subject=Unsubscribe > ―― View topic http://list.abrf.org/r/topic/4GjKRewpNnMYFwsilxTnHx Leave group <email obscured>?Subject=Unsubscribe
Thanks, Well I drafted a letter (that I would consider sending) to his Program Officer and Director of NIH and sent it to him. Wouldn't you know, I got an almost instant response imagine that... It's incredible dealing with some of these people. Guess I learned the hard, hard way - never lift a finger without a PO in hand... Thanks All...
On Fri, 2018-03-23 at 19:43 +0000, Perkins, Susanna wrote: > We have our own 'blacklist' per se... if we are struggling with a > payment, our Central Office communicates to all our facilities that > so-and-so is in arrears and if s/he reaches out to you for work, send > them to the Central Office and don't take anything from them. It's > not often it happens, but the last time, the PI was within State and > drove over immediately when he discovered his name was sent to all > the facilities. He came with credit card in hand ;-) > > Susanna Perkins > Director, Research Cores & Operations > Research Core Administration, S2-106 > Tel: (508) 856-8255 > Fax: (508) 856-2303 > E-Mail: <email obscured> > > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use > of the intended recipient(s) and may contain confidential, > proprietary and privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender immediately and destroy or > permanently delete all copies of the original message. > > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>] On Behalf Of > <email obscured> > Sent: Friday, March 23, 2018 12:56 PM > To: <email obscured> > Subject: Re: [ABRF Discussion Forum] Re: Non-payment by outside PIs > -> Any recourse? > > We have discussed a black list of institutions within our > institution. So, if we do not get paid for work done for Dr. Smith > at the U of Mars, no one can do work for the U of Mars until the bill > is paid. > > We are also not allowed to do work until we have a PO. To be honest, > there are times I start, but, like Brett, I never give them what they > requested without a PO in hand … and not just a PO number, an > official PO in the form of a pdf. I know I hate to do this paperwork > stuff and carrots always work for me … so I give them carrots too ;) > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>> on behalf of Brett > Phinney <email obscured>> > Reply-To: <email obscured>" <email obscured>> > Date: Friday, March 23, 2018 at 12:47 PM > To: <email obscured>" <email obscured>> > Subject: [EXTERNAL] Re: [ABRF Discussion Forum] Non-payment by > outside PIs -> Any recourse? > > Common problem unfortunately, at one point a few months back I > was owed over 100K from a 10-20 different people maybe? Most of the > time it's just an oversite or clerical error at the host institution, > on the rare occasion where it's malicious and they have no intention > of paying just ban them or their entire institution (if you want to > be hard core) from using you again...that's probably the only thing > you can do. > > No ABRF bad PI list as far as I know...Not sure I would encourage > that either... > > Another strategy we sometimes use is not giving the clients the > data until we have the PO or credit card number... We don't do this > frequently...but it does work. Especially when you say "hey the data > looks awesome...as soon as we get your payment info we will send it > right over" > > > Cheers > > Brett > > > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>> On Behalf Of > Perera, Anoja > Sent: Friday, March 23, 2018 6:56 AM > To: <email obscured> > Subject: Re: [ABRF Discussion Forum] Non-payment by outside PIs > -> Any recourse? > > Can you contact the institute? > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>> On Behalf Of > Andor J Kiss > Sent: Friday, March 23, 2018 8:51 AM > To: <email obscured> > Subject: [ABRF Discussion Forum] Non-payment by outside PIs -> > Any recourse? > > Hi, > > Just ran into a situation wherein an outside PI > (academic) keep delaying their PO. Long story short, it never came > and now there is no response from the PI. > > Is there any recourse in this situation? Or is this a > Institution to Institution issue at this point? > > Does ABRF maintain a "Blacklist" of bad PIs? > > Thanks, > -- > _________________________________________________________________ > ______ > _________________________________________________ > Andor J Kiss, PhD > Director - Center for Bioinformatics & Functional Genomics > 086 Pearson Hall - Miami University > 700 East High Street, Oxford > Ohio 45056 > USA > > eMAIL: <email obscured> > Telephone: +1 (513) 529-4280 > Fax: +1 (513) 529-2431 > Ring ID: andorjkiss > > URL (CBFG): https://urldefense.proofpoint.com/v2/url?u=http-3A__m > iamioh.edu_cas_academics_centers_cbfg_&d=DwIFaQ&c=WJBj9sUF1mbpVIAf3bi > u3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=qog7ACeR5C6W7dTFJB3TO854O > WtGwuMjU0F3BxMTYBA&e= > URL (CBFG Services): https://urldefense.proofpoint.com/v2/url?u=h > ttps-3A__www.scienceexchange.com_labs_center-2Dfor- > 2Dbi&d=DwIFaQ&c=WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y- > DPMs-8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=7FEURx1auu0zzCc- > QA2KQVqw337I_O4oe-2xClhLFlQ&e= > oinformatics-functional-genomics > URL (Research): https://urldefense.proofpoint.com/v2/url?u=http-3 > A__openwetware.org_wiki_User-3AAndor-5FJ- > 5FKiss&d=DwIFaQ&c=WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8 > Y-DPMs-8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=e0rEPFjKQMqsrUeOIVJs4eEV2 > EjDMrj_83cjdzVdx1U&e= > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__li > st.abrf.org_r_topic_23hIOlZmyS6vK2bSqFYVPx&d=DwIFaQ&c=WJBj9sUF1mbpVIA > f3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=03ssiwVT3jvZtmgEdkRUS6RLD > G9txjqx44GhW6vOOr8&e= > Leave group <email obscured>?Subject=Unsubscribe > > > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__li > st.abrf.org_r_topic_2BpJFyy1iInrFalIYMY0o6&d=DwIFaQ&c=WJBj9sUF1mbpVIA > f3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=H3OE9k7ojCR4XNXY8HKaoHKbe > HLb1e8UeY509tRT_Ck&e= > Leave group <email obscured>?Subject=Unsubscribe > > > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__li > st.abrf.org_r_topic_4k26g7DaHFivk7JuD08Td3&d=DwIFaQ&c=WJBj9sUF1mbpVIA > f3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=cn7nTiykosr5jWRyXqafiIw2n > nHVm6wmMZ4Zbyao57c&e= > Leave group <email obscured>?Subject=Unsubscribe > > > > > *** Only open attachments or links from trusted senders. Report > phishing to <email obscured> *** > > > > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__list.a > brf.org_r_topic_qSOeOyFTp6c90to8wQ5Q5&d=DwIFaQ&c=WJBj9sUF1mbpVIAf3biu > 3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=Q76xxpgRXGt24WEUwLP1aUceJ > nKlDTib0TdUCxCryp0&e= > Leave group <email obscured>?Subject=Unsubscribe > > > ―― > View topic http://list.abrf.org/r/topic/2IVQtA4dgPzB4OwaxEtx0z > Leave group <email obscured>?Subject=Unsubscribe >
That's the power of centralized facility management... Yan On Fri, Mar 23, 2018 at 3:43 PM, Perkins, Susanna <
<email obscured>> wrote: > We have our own 'blacklist' per se... if we are struggling with a payment, > our Central Office communicates to all our facilities that so-and-so is in > arrears and if s/he reaches out to you for work, send them to the Central > Office and don't take anything from them. It's not often it happens, but > the last time, the PI was within State and drove over immediately when he > discovered his name was sent to all the facilities. He came with credit > card in hand ;-) > > Susanna Perkins > Director, Research Cores & Operations > Research Core Administration, S2-106 > Tel: (508) 856-8255 > Fax: (508) 856-2303 > E-Mail: <email obscured> > > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential, proprietary and > privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender immediately and destroy or permanently delete all copies > of the original message. > > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>] On Behalf Of > <email obscured> > Sent: Friday, March 23, 2018 12:56 PM > To: <email obscured> > Subject: Re: [ABRF Discussion Forum] Re: Non-payment by outside PIs -> Any > recourse? > > We have discussed a black list of institutions within our institution. > So, if we do not get paid for work done for Dr. Smith at the U of Mars, no > one can do work for the U of Mars until the bill is paid. > > We are also not allowed to do work until we have a PO. To be honest, there > are times I start, but, like Brett, I never give them what they requested > without a PO in hand … and not just a PO number, an official PO in the form > of a pdf. I know I hate to do this paperwork stuff and carrots always work > for me … so I give them carrots too ;) > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>> on behalf of Brett > Phinney <email obscured>> > Reply-To: <email obscured>" <email obscured>> > Date: Friday, March 23, 2018 at 12:47 PM > To: <email obscured>" <email obscured>> > Subject: [EXTERNAL] Re: [ABRF Discussion Forum] Non-payment by outside PIs > -> Any recourse? > > Common problem unfortunately, at one point a few months back I was > owed over 100K from a 10-20 different people maybe? Most of the time it's > just an oversite or clerical error at the host institution, on the rare > occasion where it's malicious and they have no intention of paying just ban > them or their entire institution (if you want to be hard core) from using > you again...that's probably the only thing you can do. > > No ABRF bad PI list as far as I know...Not sure I would encourage that > either... > > Another strategy we sometimes use is not giving the clients the data > until we have the PO or credit card number... We don't do this > frequently...but it does work. Especially when you say "hey the data looks > awesome...as soon as we get your payment info we will send it right over" > > > Cheers > > Brett > > > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>> On Behalf Of Perera, > Anoja > Sent: Friday, March 23, 2018 6:56 AM > To: <email obscured> > Subject: Re: [ABRF Discussion Forum] Non-payment by outside PIs -> Any > recourse? > > Can you contact the institute? > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>> On Behalf Of Andor J > Kiss > Sent: Friday, March 23, 2018 8:51 AM > To: <email obscured> > Subject: [ABRF Discussion Forum] Non-payment by outside PIs -> Any > recourse? > > Hi, > > Just ran into a situation wherein an outside PI (academic) keep > delaying their PO. Long story short, it never came and now there is no > response from the PI. > > Is there any recourse in this situation? Or is this a Institution > to Institution issue at this point? > > Does ABRF maintain a "Blacklist" of bad PIs? > > Thanks, > -- > ____________________________________________________________ > ___________ > _________________________________________________ > Andor J Kiss, PhD > Director - Center for Bioinformatics & Functional Genomics > 086 Pearson Hall - Miami University > 700 East High Street, Oxford > Ohio 45056 > USA > > eMAIL: <email obscured> > Telephone: +1 (513) 529-4280 > Fax: +1 (513) 529-2431 > Ring ID: andorjkiss > > URL (CBFG): https://urldefense.proofpoint. > com/v2/url?u=http-3A__miamioh.edu_cas_academics_centers_cbfg_&d=DwIFaQ&c= > WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x-1vb5owjfVD7rXnFGmarcC1c2eHAajv > dMx5wRNNfko&s=qog7ACeR5C6W7dTFJB3TO854OWtGwuMjU0F3BxMTYBA&e= > URL (CBFG Services): https://urldefense.proofpoint. > com/v2/url?u=https-3A__www.scienceexchange.com_labs_ > center-2Dfor-2Dbi&d=DwIFaQ&c=WJBj9sUF1mbpVIAf3biu3CPHX4MeRj > Y_w4DerPlOmhQ&r=kGmC8Y-DPMs-8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x- > 1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=7FEURx1auu0zzCc- > QA2KQVqw337I_O4oe-2xClhLFlQ&e= > oinformatics-functional-genomics > URL (Research): https://urldefense.proofpoint.com/v2/url?u=http-3A__ > openwetware.org_wiki_User-3AAndor-5FJ-5FKiss&d=DwIFaQ&c= > WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x-1vb5owjfVD7rXnFGmarcC1c2eHAajv > dMx5wRNNfko&s=e0rEPFjKQMqsrUeOIVJs4eEV2EjDMrj_83cjdzVdx1U&e= > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__list. > abrf.org_r_topic_23hIOlZmyS6vK2bSqFYVPx&d=DwIFaQ&c= > WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x-1vb5owjfVD7rXnFGmarcC1c2eHAajv > dMx5wRNNfko&s=03ssiwVT3jvZtmgEdkRUS6RLDG9txjqx44GhW6vOOr8&e= > Leave group <email obscured>?Subject=Unsubscribe > > > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__list. > abrf.org_r_topic_2BpJFyy1iInrFalIYMY0o6&d=DwIFaQ&c= > WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x-1vb5owjfVD7rXnFGmarcC1c2eHAajv > dMx5wRNNfko&s=H3OE9k7ojCR4XNXY8HKaoHKbeHLb1e8UeY509tRT_Ck&e= > Leave group <email obscured>?Subject=Unsubscribe > > > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__list. > abrf.org_r_topic_4k26g7DaHFivk7JuD08Td3&d=DwIFaQ&c= > WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x-1vb5owjfVD7rXnFGmarcC1c2eHAajv > dMx5wRNNfko&s=cn7nTiykosr5jWRyXqafiIw2nnHVm6wmMZ4Zbyao57c&e= > Leave group <email obscured>?Subject=Unsubscribe > > > > > *** Only open attachments or links from trusted senders. Report > phishing to <email obscured> *** > > > > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__list. > abrf.org_r_topic_qSOeOyFTp6c90to8wQ5Q5&d=DwIFaQ&c= > WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs- > 8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x-1vb5owjfVD7rXnFGmarcC1c2eHAajv > dMx5wRNNfko&s=Q76xxpgRXGt24WEUwLP1aUceJnKlDTib0TdUCxCryp0&e= > Leave group <email obscured>?Subject=Unsubscribe > > > ―― > View topic http://list.abrf.org/r/topic/2IVQtA4dgPzB4OwaxEtx0z > Leave group <email obscured>?Subject=Unsubscribe > > -- Yan Wang, Ph.D. Director, Proteomics Core Facility 2225 Bioscience Research Building University of Maryland 4066 Campus Drive College Park, MD 20742 (301)405-6764 Office and lab location: 0109/0111 Biology Psychology Building 4094 Campus Drive College Park, MD 20742
Francis, are you talking about Ralph Smith of University of Alba Mons, Mars???
That guy is a total dead-beat!! Beware!!
On 3/23/18, 2:43 PM, "ABRF Discussion Forum on behalf of Perkins, Susanna"
<email obscured> on behalf of <email obscured>> wrote:
We have our own 'blacklist' per se... if we are struggling with a payment,
our Central Office communicates to all our facilities that so-and-so is in
arrears and if s/he reaches out to you for work, send them to the Central
Office and don't take anything from them. It's not often it happens, but the
last time, the PI was within State and drove over immediately when he
discovered his name was sent to all the facilities. He came with credit card
in hand ;-)
Susanna Perkins
Director, Research Cores & Operations
Research Core Administration, S2-106
Tel: (508) 856-8255
Fax: (508) 856-2303
E-Mail: <email obscured>
Confidentiality Notice:
This e-mail message, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential, proprietary and privileged
information. Any unauthorized review, use, disclosure or distribution is
prohibited. If you are not the intended recipient, please contact the sender
immediately and destroy or permanently delete all copies of the original
message.
-----Original Message-----
From: ABRF Discussion Forum <email obscured>] On Behalf Of
<email obscured>
Sent: Friday, March 23, 2018 12:56 PM
To: <email obscured>
Subject: Re: [ABRF Discussion Forum] Re: Non-payment by outside PIs -> Any
recourse?
We have discussed a black list of institutions within our institution. So,
if we do not get paid for work done for Dr. Smith at the U of Mars, no one can
do work for the U of Mars until the bill is paid.
We are also not allowed to do work until we have a PO. To be honest, there
are times I start, but, like Brett, I never give them what they requested
without a PO in hand … and not just a PO number, an official PO in the form of
a pdf. I know I hate to do this paperwork stuff and carrots always work for me
… so I give them carrots too ;)
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> on behalf of Brett Phinney
<email obscured>>
Reply-To: <email obscured>" <email obscured>>
Date: Friday, March 23, 2018 at 12:47 PM
To: <email obscured>" <email obscured>>
Subject: [EXTERNAL] Re: [ABRF Discussion Forum] Non-payment by outside PIs
-> Any recourse?
Common problem unfortunately, at one point a few months back I was owed
over 100K from a 10-20 different people maybe? Most of the time it's just an
oversite or clerical error at the host institution, on the rare occasion where
it's malicious and they have no intention of paying just ban them or their
entire institution (if you want to be hard core) from using you
again...that's probably the only thing you can do.
No ABRF bad PI list as far as I know...Not sure I would encourage that
either...
Another strategy we sometimes use is not giving the clients the data
until we have the PO or credit card number... We don't do this frequently...but
it does work. Especially when you say "hey the data looks awesome...as soon as
we get your payment info we will send it right over"
Cheers
Brett
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of Perera,
Anoja
Sent: Friday, March 23, 2018 6:56 AM
To: <email obscured>
Subject: Re: [ABRF Discussion Forum] Non-payment by outside PIs -> Any
recourse?
Can you contact the institute?
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of Andor J
Kiss
Sent: Friday, March 23, 2018 8:51 AM
To: <email obscured>
Subject: [ABRF Discussion Forum] Non-payment by outside PIs -> Any
recourse?
Hi,
Just ran into a situation wherein an outside PI (academic) keep
delaying their PO. Long story short, it never came and now there is no response
from the PI.
Is there any recourse in this situation? Or is this a Institution to
Institution issue at this point?
Does ABRF maintain a "Blacklist" of bad PIs?
Thanks,
--
_______________________________________________________________________
_________________________________________________
Andor J Kiss, PhD
Director - Center for Bioinformatics & Functional Genomics
086 Pearson Hall - Miami University
700 East High Street, Oxford
Ohio 45056
USA
eMAIL: <email obscured>
Telephone: +1 (513) 529-4280
Fax: +1 (513) 529-2431
Ring ID: andorjkiss
URL (CBFG):
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oinformatics-functional-genomics
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We have our own 'blacklist' per se... if we are struggling with a payment, our Central Office communicates to all our facilities that so-and-so is in arrears and if s/he reaches out to you for work, send them to the Central Office and don't take anything from them. It's not often it happens, but the last time, the PI was within State and drove over immediately when he discovered his name was sent to all the facilities. He came with credit card in hand ;-) Susanna Perkins Director, Research Cores & Operations Research Core Administration, S2-106 Tel: (508) 856-8255 Fax: (508) 856-2303 E-Mail: <email obscured> Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender immediately and destroy or permanently delete all copies of the original message.
-----Original Message-----
From: ABRF Discussion Forum <email obscured>] On Behalf Of
<email obscured>
Sent: Friday, March 23, 2018 12:56 PM
To: <email obscured>
Subject: Re: [ABRF Discussion Forum] Re: Non-payment by outside PIs -> Any
recourse?
We have discussed a black list of institutions within our institution. So, if
we do not get paid for work done for Dr. Smith at the U of Mars, no one can do
work for the U of Mars until the bill is paid.
We are also not allowed to do work until we have a PO. To be honest, there are
times I start, but, like Brett, I never give them what they requested without a
PO in hand … and not just a PO number, an official PO in the form of a pdf. I
know I hate to do this paperwork stuff and carrots always work for me … so I
give them carrots too ;)
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> on behalf of Brett Phinney
<email obscured>>
Reply-To: <email obscured>" <email obscured>>
Date: Friday, March 23, 2018 at 12:47 PM
To: <email obscured>" <email obscured>>
Subject: [EXTERNAL] Re: [ABRF Discussion Forum] Non-payment by outside PIs ->
Any recourse?
Common problem unfortunately, at one point a few months back I was owed
over 100K from a 10-20 different people maybe? Most of the time it's just an
oversite or clerical error at the host institution, on the rare occasion where
it's malicious and they have no intention of paying just ban them or their
entire institution (if you want to be hard core) from using you
again...that's probably the only thing you can do.
No ABRF bad PI list as far as I know...Not sure I would encourage that
either...
Another strategy we sometimes use is not giving the clients the data until
we have the PO or credit card number... We don't do this frequently...but it
does work. Especially when you say "hey the data looks awesome...as soon as we
get your payment info we will send it right over"
Cheers
Brett
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of Perera, Anoja
Sent: Friday, March 23, 2018 6:56 AM
To: <email obscured>
Subject: Re: [ABRF Discussion Forum] Non-payment by outside PIs -> Any
recourse?
Can you contact the institute?
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of Andor J Kiss
Sent: Friday, March 23, 2018 8:51 AM
To: <email obscured>
Subject: [ABRF Discussion Forum] Non-payment by outside PIs -> Any
recourse?
Hi,
Just ran into a situation wherein an outside PI (academic) keep delaying
their PO. Long story short, it never came and now there is no response from the
PI.
Is there any recourse in this situation? Or is this a Institution to
Institution issue at this point?
Does ABRF maintain a "Blacklist" of bad PIs?
Thanks,
--
_______________________________________________________________________
_________________________________________________
Andor J Kiss, PhD
Director - Center for Bioinformatics & Functional Genomics
086 Pearson Hall - Miami University
700 East High Street, Oxford
Ohio 45056
USA
eMAIL: <email obscured>
Telephone: +1 (513) 529-4280
Fax: +1 (513) 529-2431
Ring ID: andorjkiss
URL (CBFG):
https://urldefense.proofpoint.com/v2/url?u=http-3A__miamioh.edu_cas_academics_centers_cbfg_&d=DwIFaQ&c=WJBj9sUF1mbpVIAf3biu3CPHX4MeRjY_w4DerPlOmhQ&r=kGmC8Y-DPMs-8zQyfdP7sDzvCno4Bgua6y8e7K43bg8&m=x-1vb5owjfVD7rXnFGmarcC1c2eHAajvdMx5wRNNfko&s=qog7ACeR5C6W7dTFJB3TO854OWtGwuMjU0F3BxMTYBA&e=
URL (CBFG Services):
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oinformatics-functional-genomics
URL (Research):
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This is a crime, it is called Theft of Services. Is the PI still at the
institution?
We had this problem – in our case a company contracted us. I finally had one of
the WashU lawyers write a letter to the company. In this case, that’s all it
took.
On 3/23/18, 8:56 AM, "ABRF Discussion Forum on behalf of Perera, Anoja"
<email obscured> on behalf of <email obscured>> wrote:
Can you contact the institute?
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of Andor J Kiss
Sent: Friday, March 23, 2018 8:51 AM
To: <email obscured>
Subject: [ABRF Discussion Forum] Non-payment by outside PIs -> Any
recourse?
Hi,
Just ran into a situation wherein an outside PI (academic) keep delaying
their PO. Long story short, it never came and now there is no response from the
PI.
Is there any recourse in this situation? Or is this a Institution to
Institution issue at this point?
Does ABRF maintain a "Blacklist" of bad PIs?
Thanks,
--
_______________________________________________________________________
_________________________________________________
Andor J Kiss, PhD
Director - Center for Bioinformatics & Functional Genomics
086 Pearson Hall - Miami University
700 East High Street, Oxford
Ohio 45056
USA
eMAIL: <email obscured>
Telephone: +1 (513) 529-4280
Fax: +1 (513) 529-2431
Ring ID: andorjkiss
URL (CBFG): http://miamioh.edu/cas/academics/centers/cbfg/
URL (CBFG Services): https://www.scienceexchange.com/labs/center-for-bi
oinformatics-functional-genomics
URL (Research): http://openwetware.org/wiki/User:Andor_J_Kiss
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