Awesome!
Sent from my iPhone
Rest of post
> On Nov 8, 2018, at 3:28 PM, Kevin Feldheim <email obscured>> wrote:
>
> Hi Anoja,
>
> We have several Bio-Rad C1000 touch thermal cyclers, which tend to be
> reliable:
>
> http://www.bio-rad.com/en-us/product/c1000-touch-thermal-cycler?ID=LGTW9415
>
> We recently got rid of four Eppendorf Mastercyclers because we had to keep
> sending them in for repair. These were older models however, and I don't
> have experience with their newer ones.
>
> Best,
> Kevin
>
>> On Thu, Nov 8, 2018 at 2:03 PM, Perera, Anoja <email obscured>> wrote:
>>
>> Hello,
>>
>> We are considering replacing our old thermocyclers. We mostly use them for
>> PCR; Sanger Sequencing and NGS. Currently, we have Bio-Rad tetrads. What
>> are you using in your cores? Any favorites?
>>
>> Thanks,
>>
>> Anoja
>>
>> ――
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>> Leave group <email obscured>?Subject=Unsubscribe
>>
>>
>
>
> --
>
***************************************************************************************************************
> Kevin Feldheim
> A. Watson Armour III Manager of the Pritzker Laboratory for Molecular
> Systematics and Evolution
> Field Museum
> 1400 S. Lake Shore Drive
> Chicago, IL 60605
> -----------------------------------------------------------
> phone: 312-665-7770
> fax: 312-665-7754
> e-mail: <email obscured>
>
***************************************************************************************************************
>
> ――
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>
>
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>
> *** Only open attachments or links from trusted senders. Report phishing to
<email obscured> ***
Hi Kevin and Anoja,
After reading Kevin’s post, I though I would share my experience. This doesn’t
address thermo cyclers directly, but rather Eppendorf mean time between failure
for other Eppendorf products I have direct experience with.
Example: I purchased an Eppendorf MixMate originally in 2011. It underwent two
recalls which required me to send the unit back and wait for them to send its
replacement. The recall was due to a fire hazard and they wanted to make
certain the recalled units were removed from the field. So I can understand why
they waited to receive the defective units before sending out replacements.
The replacement unit was itself recalled about two years later and I had to do
this same dance again to get the replacement. I thought, this isn’t bad, every
two years I’m getting a new MixMate!
However, this year the MixMate stopped mixing (sad face emoji) and was the
result of either or both of two electronic boards which would need to be
diagnosed at the repair center for $400 and then repaired for hundreds of
dollars depending on which board (or both) was bad. Based on past experience,
I had thought that Eppendorf engineering was the best but now I’m not so sure.
I expected this instrument to last at least 10 years, especially given the
fairly high cost of these units. Its physical design and functionality
perfectly fit my needs, but unfortunately it had a short time to failure. By
contrast we have a decades old Eppendorf mixer of similar footprint but no
electronic boards and a single speed. It still works.
In the end I decided to purchase a new replacement - equivalent in function to
the MixMate - but from another manufacturer for slightly more than the cost of
repairing the MixMate itself and at a cost very significantly less than the
cost of a new Mixmate from Eppendorf.
From what Kevin writes and my experience with other Eppendorf devices, it seems
that cost of repair and down time might have to be considered for budgeting
purposes in addition to the initial purchase price.
Cheers,
Brian
Brian Hampton
Program Director
Center for Vascular and Inflammatory Diseases
University of Maryland School of Medicine
BRB Room 7-018
655 West Baltimore St
Baltimore MD. 21201
On Nov 8, 2018, at 3:27 PM, Kevin Feldheim
<email obscured><email obscured>>> wrote:
CAUTION: This message originated from a non UMB, UMSOM, FPI, or UMMS email
system. Whether the sender is known or not known, hover over any links before
clicking and use caution opening attachments.
Hi Anoja,
We have several Bio-Rad C1000 touch thermal cyclers, which tend to be
reliable:
http://www.bio-rad.com/en-us/product/c1000-touch-thermal-cycler?ID=LGTW9415
We recently got rid of four Eppendorf Mastercyclers because we had to keep
sending them in for repair. These were older models however, and I don't
have experience with their newer ones.
Best,
Kevin
On Thu, Nov 8, 2018 at 2:03 PM, Perera, Anoja
<email obscured><email obscured>>> wrote:
Hello,
We are considering replacing our old thermocyclers. We mostly use them for
PCR; Sanger Sequencing and NGS. Currently, we have Bio-Rad tetrads. What
are you using in your cores? Any favorites?
Thanks,
Anoja
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Rest of post
--
***************************************************************************************************************
Kevin Feldheim
A. Watson Armour III Manager of the Pritzker Laboratory for Molecular
Systematics and Evolution
Field Museum
1400 S. Lake Shore Drive
Chicago, IL 60605
-----------------------------------------------------------
phone: 312-665-7770
fax: 312-665-7754
e-mail: <email obscured><email obscured>>
***************************************************************************************************************
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We like Kanbanchi.
C
On Thu, Nov 8, 2018 at 4:17 PM Fred W. Kolling IV <
<email obscured>> wrote:
> This is great information, thank you Nathan!
>
> ________________________________
> From: ABRF Discussion Forum <email obscured>> on behalf of bivensn <
> <email obscured>>
> Sent: Thursday, November 8, 2018 3:44:23 PM
> To: <email obscured>
> Subject: Re: [ABRF Discussion Forum] Project management solutions
>
> Fred,
>
> For project status tracking and assignment, our group uses the on-line
> solution from LeanKit (
>
https://urldefense.proofpoint.com/v2/url?u=https-3A__na01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Fleankit.com-252F-26amp-3Bdata-3D02-257C01-257Cfred.w.kolling.iv-2540dartmouth.edu-257Cf94516ba0f564d93a37a08d645baf955-257C995b093648d640e5a31ebf689ec9446f-257C0-257C0-257C636773066696228887-26amp-3Bsdata-3DiNT3jV1KBArErerUoXKMEj2MGIelGkETceXymR9KxMc-253D-26amp-3Breserved-3D0&d=DwIFaQ&c=slrrB7dE8n7gBJbeO0g-IQ&r=VMAcdWMgLj27ywTQG-CkDw&m=Ffjlb4YjSmMl7H2hp3OumvHqJrJoW-4EKXHRc6oLPzI&s=Cxz14G-9aV0qLCy7nxkwkh6r00iXuLylQVKoRWRMeQQ&e=)
Rest of post
> . The initial set-up requires some thought as to how you want to track the
> workflow but once the staff got use to the system they really like it. It
> is very easy to pull up the board during weekly staff meeting and know the
> status of each project at a glance. The built in project tracking tools
> allow you to follow run queries on project metrics. You can also attach
> documents that become available to everyone.
>
> I should say that this software was not designed for a science lab but I
> found it easily adaptable.
>
> Best,
>
> Nathan
> ――
> View topic
>
https://urldefense.proofpoint.com/v2/url?u=https-3A__na01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Flist.abrf.org-252Fr-252Ftopic-252FOsVif8lDWA8AatCC2897L-26amp-3Bdata-3D02-257C01-257Cfred.w.kolling.iv-2540dartmouth.edu-257Cf94516ba0f564d93a37a08d645baf955-257C995b093648d640e5a31ebf689ec9446f-257C0-257C0-257C636773066696238895-26amp-3Bsdata-3DibwEXZeNY-252FkT0RVMU-252F-252FQ87cFrN0l78-252FoqQPkAVvRLps-253D-26amp-3Breserved-3D0&d=DwIFaQ&c=slrrB7dE8n7gBJbeO0g-IQ&r=VMAcdWMgLj27ywTQG-CkDw&m=Ffjlb4YjSmMl7H2hp3OumvHqJrJoW-4EKXHRc6oLPzI&s=Mi3ArGT4cIKZ4-7z4mV6qm5tO4XLpQoOG7XTsfOFNcg&e=
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--
Christine Vogel, PhD
New York University - Department of Biology
Center for Genomics & Systems Biology
12 Waverly Pl - Office #403
New York, NY 10003
phone: +1-212-998-3976
https://sites.google.com/a/nyu.edu/vogel-lab/home
“If your dreams don't scare you, they are not big enough” Ellen Johnson
Sirleaf
Fred,
For project status tracking and assignment, our group uses the on-line solution
from LeanKit (https://leankit.com/) . The initial set-up requires some thought
as to how you want to track the workflow but once the staff got use to the
system they really like it. It is very easy to pull up the board during weekly
staff meeting and know the status of each project at a glance. The built in
project tracking tools allow you to follow run queries on project metrics. You
can also attach documents that become available to everyone.
I should say that this software was not designed for a science lab but I found
it easily adaptable.
Thanks Kevin! This is really good information. We have been considering C1000s.
We already have few of those and few T100s in the lab and like them.
Rest of post
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of Kevin Feldheim
Sent: Thursday, November 08, 2018 2:28 PM
To: <email obscured>
Subject: Re: [ABRF Discussion Forum] Thermocyclers
**CAUTION: Non-Stowers email**
Hi Anoja,
We have several Bio-Rad C1000 touch thermal cyclers, which tend to be
reliable:
http://www.bio-rad.com/en-us/product/c1000-touch-thermal-cycler?ID=LGTW9415
We recently got rid of four Eppendorf Mastercyclers because we had to keep
sending them in for repair. These were older models however, and I don't have
experience with their newer ones.
Best,
Kevin
On Thu, Nov 8, 2018 at 2:03 PM, Perera, Anoja <email obscured>> wrote:
> Hello,
>
> We are considering replacing our old thermocyclers. We mostly use them
> for PCR; Sanger Sequencing and NGS. Currently, we have Bio-Rad
> tetrads. What are you using in your cores? Any favorites?
>
> Thanks,
>
> Anoja
>
> ――
> View topic http://list.abrf.org/r/topic/70lInteDZgoa74t4n0Mii8
> Leave group <email obscured>?Subject=Unsubscribe
>
>
--
***************************************************************************************************************
Kevin Feldheim
A. Watson Armour III Manager of the Pritzker Laboratory for Molecular
Systematics and Evolution Field Museum
1400 S. Lake Shore Drive
Chicago, IL 60605
-----------------------------------------------------------
phone: 312-665-7770
fax: 312-665-7754
e-mail: <email obscured>
***************************************************************************************************************
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Hello Anoja,
We've been happy with our Eppendorf Mastercyclers. We like the ability to
daisy-chain the units and operate from a single controller.
Hi Anoja,
We have several Bio-Rad C1000 touch thermal cyclers, which tend to be
reliable:
http://www.bio-rad.com/en-us/product/c1000-touch-thermal-cycler?ID=LGTW9415
We recently got rid of four Eppendorf Mastercyclers because we had to keep
sending them in for repair. These were older models however, and I don't
have experience with their newer ones.
Rest of post
Best,
Kevin
On Thu, Nov 8, 2018 at 2:03 PM, Perera, Anoja <email obscured>> wrote:
> Hello,
>
> We are considering replacing our old thermocyclers. We mostly use them for
> PCR; Sanger Sequencing and NGS. Currently, we have Bio-Rad tetrads. What
> are you using in your cores? Any favorites?
>
> Thanks,
>
> Anoja
>
> ――
> View topic http://list.abrf.org/r/topic/70lInteDZgoa74t4n0Mii8
> Leave group <email obscured>?Subject=Unsubscribe
>
>
--
***************************************************************************************************************
Kevin Feldheim
A. Watson Armour III Manager of the Pritzker Laboratory for Molecular
Systematics and Evolution
Field Museum
1400 S. Lake Shore Drive
Chicago, IL 60605
-----------------------------------------------------------
phone: 312-665-7770
fax: 312-665-7754
e-mail: <email obscured>
***************************************************************************************************************
Hi Fred,
We use a mix of internal LIMS, SharePoint and Atlassian tools. You might want
to look at Atlassian tools. Perhaps something like Confluence will work for
you.
Rest of post
Best,
Anoja
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of Fred W. Kolling
IV
Sent: Thursday, November 08, 2018 2:09 PM
To: ABRF Discussion List <email obscured>) <email obscured>>
Subject: [ABRF Discussion Forum] Project management solutions
**CAUTION: Non-Stowers email**
Hi Everyone,
As we grow our user base, we are looking for better solutions to manage
projects and the tasks associated with each work flow. Solutions that track who
is working on what, and the status of each project are preferred.
What are you all using? Commercial project management software/freeware?
Elaborate white board charts?
Any and all ideas are welcome.
Thanks!
Fred
Sent from my mobile device. Please excuse the brevity and typos.
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Hi Everyone,
As we grow our user base, we are looking for better solutions to manage
projects and the tasks associated with each work flow. Solutions that track who
is working on what, and the status of each project are preferred.
What are you all using? Commercial project management software/freeware?
Elaborate white board charts?
Any and all ideas are welcome.
Thanks!
Fred
Sent from my mobile device. Please excuse the brevity and typos.
Hello,
We are considering replacing our old thermocyclers. We mostly use them for PCR;
Sanger Sequencing and NGS. Currently, we have Bio-Rad tetrads. What are you
using in your cores? Any favorites?
Dear Andor,
In our hands, the two systems are equivalent for small-sized samples.
Rest of post
Best,
Claire
-----Original Message-----
From: ABRF Discussion Forum <email obscured>> On Behalf Of Andor J Kiss
Sent: Monday, November 05, 2018 1:52 PM
To: <email obscured>
Subject: [ABRF Discussion Forum] BluePippin VS PippinPrep | Same for small
fragments?
Hi All,
We have a PippinPrep (and are happy with it). We have recently looked at the
BluePippin for large fragments. One thing that SAGE has suggested is that the
BluePippin can do the exact same as the PippinPrep + larger fragments
(depending on which cassette is used).
Anyone have any practical experience with this? Is this in fact true, or is
the PippinPrep still better for between 90 ~ 1500 bp?
Thanks,
--
_____________________________________________________________________________Andor
J Kiss, PhDDirector - Center for Bioinformatics & Functional Genomics086
Pearson Hall - Miami University700 East High Street, OxfordOhio 45056USA
eMAIL: <email obscured> Telephone: +1 (513) 529-4280Fax: +1 (513)
529-2431Ring ID: andorjkiss URL (CBFG):
http://miamioh.edu/cas/academics/centers/cbfg/ URL (CBFG Services):
https://www.scienceexchange.com/labs/center-for-bioinformatics-functional-genomicsURL
(Research): http://openwetware.org/wiki/User:Andor_J_Kiss
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Hi All,
We have a PippinPrep (and are happy with it). We have recently
looked at the BluePippin for large fragments. One thing that SAGE has
suggested is that the BluePippin can do the exact same as the
PippinPrep + larger fragments (depending on which cassette is used).
Anyone have any practical experience with this? Is this in fact true,
or is the PippinPrep still better for between 90 ~ 1500 bp?
Rest of post
Thanks,
--
_____________________________________________________________________________Andor
J Kiss, PhDDirector - Center for Bioinformatics & Functional Genomics086
Pearson Hall - Miami University700 East High Street, OxfordOhio 45056USA
eMAIL: <email obscured> Telephone: +1 (513) 529-4280Fax: +1 (513)
529-2431Ring ID: andorjkiss
URL (CBFG): http://miamioh.edu/cas/academics/centers/cbfg/ URL (CBFG Services):
https://www.scienceexchange.com/labs/center-for-bioinformatics-functional-genomicsURL
(Research): http://openwetware.org/wiki/User:Andor_J_Kiss
Hi Subi,
I think of two options:
1) Synthesis of the fully protected peptide (with N-terminal Boc group instead
of Fmoc, and free carboxyl group at the C terminus) using either a Cl-Trt resin
or HMPB resin. Then coupling the amine to the C terminus with a solution of the
amine in an inert solvent. At the last step then the complete deprotection as
usual.
or
2) Using an HMBA resin and cleave the peptide with a methanolic solution of the
amine.
Cheers,
Dirk
Dr. Dirk Winkler
Senior Manager Peptide Facility
Kinexus Bioinformatics Corporation
8755 Ash Street, Suite 1
Vancouver, BC
V6P 6T3, Canada
<email obscured>
Tel. 604-323-2547 Ext.5
Fax 604-323-2548
http://www.kinexus.ca/ourServices/proteinAndPeptide/index.html
Hi,
what is your C-terminal residue?
Rest of post
Best
Stephan
-----Ursprüngliche Nachricht-----
Von: ABRF Discussion Forum <email obscured>] Im Auftrag von OKARVI,
SUBHANI MOHAMMAD
Gesendet: Montag, 5. November 2018 13:31
An: <email obscured>
Betreff: Re: [ABRF Discussion Forum] Fmoc-based SPPS help
Hi there,
I am planning to synthesize a peptide with C-terminal NHEt. I mainly used rink
amide resin to construct C-terminal amide but do not have any experience with
NHEt terminal group. Any help in this regard will be highly appreciated.
Thank you.
Subi Okarvi
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