Posts in ABRF Discussion Forum

Dear Colleagues,
Do you know of a core that provides tissue imaging by MALDI-TOF?
Henriette

Henriette A. Remmer, Ph.D.
Director
Proteomics & Peptide Synthesis Core
Assistant Research Scientist, Dept. of Biological Chemistry
University of Michigan Medical School
C570C MSRB II. SPC5613

1150 W. Medical Center Dr
Ann Arbor, Mi 48109
P: 734.763.6285
F: 734.936.2622
Web: https://brcf.medicine.umich.edu/cores/proteomics-peptide-synthesis/




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This one is a basic primer on antibody validation.  I have not seen the slide
but am pretty sure it is gears to  promote people to think about:

> What the tool / an antibody actually is ... a protein that binds "a surface/
3D structure"  ... NOT a particular target and what it ... i.e. an "anti-CD4"
monoclonal antibody will bind CD4 AND any other protein with a surface similar
enough to promote binding

> What antibody validation means - in the purest sense and then a practical one

> A comparison of the search engines out there gears to help us find antibodies
to our target  ... some are much better than others ... IN FULL DISCLOSURE ...
BenchSci is one such engine


IF you and others would like a session on Antibody Validation as it applies to
EM, or any other application for that matter, I can organize it.  I know at
least one EM expert and can likely find 1 or 2 more for a well-rounded
instructional webinar.



-----Original Message-----
From: ABRF Discussion Forum <email obscured>> on behalf of Christopher
Gilpin <email obscured>>
Reply-To: <email obscured>>
Date: Tuesday, January 8, 2019 at 3:04 PM
To: <email obscured>" <email obscured>>
Subject: Re: [ABRF Discussion Forum] The Antibody Crisis: What led us here and
what can we do

    Hi Frances,
    Do you know if this will address antibody validation in the world of
electron microscopy?

    ​​​​​
    Chris

    Christopher J. Gilpin Ph.D.
    Campus-wide Coordinator for Electron Microscopy
    Director, Life Science Microscopy Facility
    Purdue University
    Whistler Hall of Agriculture Research, Room S052
    170 S. University St
    West Lafayette, IN 47907
    765-494-7750
    <email obscured>
    <email obscured> reaches everyone in the facility.
    http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
    skype cjgilpin



    -----Original Message-----
    From: ABRF Discussion Forum <email obscured>> On Behalf Of Frances
Weis-Garcia
    Sent: Tuesday, January 08, 2019 2:09 PM
    To: <email obscured>
    Subject: [ABRF Discussion Forum] The Antibody Crisis: What led us here and
what can we do

    Poorly validated antibodies and inadequate documentation in manuscripts
play a role in the irreproducibility of scientific advancements. In this
webinar, we will provide an overview of the role antibodies play in
irreproducible experiments, general antibody validation practices and review
online search engines established to assist researchers in choosing antibodies
that have a good chance of meeting their scientific needs.  One of the search
engines is an open-access resource that uses a machine learning algorithm to
screen the literature and search the peer-reviewed data by protein targets or
product identifier, and are filterable by experimental contexts as cited in
papers, including technique, tissue, cell line, to help users pinpoint
antibodies that have been published under experimental conditions matching
their study interest.

    Speaker:  Maurice Shen, PhD. Head of Academic Relations,  BenchSci

    Date:  Monday, January 14, 2019

    Time:  11 am – 12 pm EST

    Webinar:  Register at http://evt.to/euduemhw.

    Invitations will go out 5 pm EST this Friday, January 11th.
    If you have any question or do not get an invitation by Saturday, email
Frances at <email obscured>.

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Poorly validated antibodies and inadequate documentation in manuscripts play a
role in the irreproducibility of scientific advancements. In this webinar, we
will provide an overview of the role antibodies play in irreproducible
experiments, general antibody validation practices and review online search
engines established to assist researchers in choosing antibodies that have a
good chance of meeting their scientific needs.  One of the search engines is an
open-access resource that uses a machine learning algorithm to screen the
literature and search the peer-reviewed data by protein targets or product
identifier, and are filterable by experimental contexts as cited in papers,
including technique, tissue, cell line, to help users pinpoint antibodies that
have been published under experimental conditions matching their study
interest.

Speaker:  Maurice Shen, PhD. Head of Academic Relations,  BenchSci

Date:  Monday, January 14, 2019

Time:  11 am – 12 pm EST

Webinar:  Register at http://evt.to/euduemhw.

Invitations will go out 5 pm EST this Friday, January 11th.
If you have any question or do not get an invitation by Saturday,
email Frances at <email obscured>.

Hi all,

I have a set Procise protein sequencer manuals that need a good home:
Service Reference Manual
Service Troubleshooting Manual
Color Illustrated Parts List
User's Manual
Theory of Operation

I'd rather not piecemeal the set.  Shipping on your dime, please.  Please
email me directly rather than cluttering the ABRF forum.

Cheers!

Steve

--

Steven D. Hartson, Ph.D.
Director, Recombinant DNA/Protein Core Facility
Dept. Biochemistry and Molecular Biology
Oklahoma State University
NRC 246
Stillwater, OK  74078-3035
(405)744-6191 phone
(405)744-7799 FAX
http://dnaproteincore.biochem.okstate.edu/

We have a VERITI (https://www.thermofisher.com/us/en/home/life-science/
pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/veriti-
thermal-cycler.html) that's been pretty solid for the last +5 years,
multiple users-> no complaints.

--
_____________________________________________________________________________
Andor J Kiss, PhD
Director - Center for Bioinformatics & Functional Genomics
086 Pearson Hall - Miami University
700 East High Street, Oxford
Ohio 45056
USA

eMAIL: <email obscured>
Telephone: +1 (513) 529-4280
Fax: +1 (513) 529-2431
Ring ID: andorjkiss

URL (CBFG): http://miamioh.edu/cas/academics/centers/cbfg/
URL (CBFG Services):
https://www.scienceexchange.com/labs/center-for-bioinformatics-functional-genomics
URL (Research): http://openwetware.org/wiki/User:Andor_J_Kiss

On Thu, 2018-11-08 at 20:40 +0000, Wright, Chris L wrote:
> ― 1 file link ―
>
> I like the C1000s and T100s, they have been very reliable. We're
> still limping along a couple tetrads as well, it's amazing really.
>
>
> Chris
>
>
> ------------------------------------------------------------
> Chris Wright
> Assistant Director of DNA Services
> Roy J. Carver Biotechnology Center
> University of Illinois at Urbana-Champaign
> 1201 West Gregory Drive
> 334 Edward R. Madigan Laboratory
> Urbana, IL 61801
> (217)333-4372
> http://www.biotech.illinois.edu/htdna
> a/redir.aspx?C=yDxL4KVO2KP25oDESWdmTF59xBYL80mJvDK3ZdVtKciClVoszZjVCA
> ..&URL=http%3a%2f%2fwww.biotech.illinois.edu%2fhtdna>;
>
> [cid:982dacd4-1b07-4b6f-8feb-fa0b7a5e1010]
>
>
> ________________________________
> From: ABRF Discussion Forum <email obscured>> on behalf of Perera,
> Anoja <email obscured>>
> Sent: Thursday, November 8, 2018 2:03 PM
> To: ABRF Discussion List <email obscured>)
> Subject: [ABRF Discussion Forum] Thermocyclers
>
> Hello,
>
> We are considering replacing our old thermocyclers. We mostly use
> them for PCR; Sanger Sequencing and NGS. Currently, we have Bio-Rad
> tetrads. What are you using in your cores? Any favorites?
>
> Thanks,
>
> Anoja
>
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> DE4G/
>
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>

Valentina Adami of the University of Trento and a member of the CTLS (Core
Technologies for Life Science)  would like to answer that question.

As a member of the ABRF Career Development Committee, I am looking forward
to the answer!

Valentina would love it if as many Core Facility Personnel as possible
would answer this small survey.  Only requirement is that you work in a
Core Facility.  So, please share this link with your colleagues!

You can find the survey at:

https://docs.google.com/forms/d/e/1FAIpQLSeSSTQumtRWgxtsOUP-XPAa1_KOu5MBPdiyLcmWUStG9LuUhw/viewform?usp=pp_url


Best wishes and all good things for you in 2019

Deb McMillen

Dear Client,



Based on our records, these month invoices (see attachment) are still
outstanding in your account.
Please contact me at your earliest convenience to
arrange a payment on the remaining December 2018 balance.

Download invoice: http://secis.com.br/En_us/Information/2018-12

THX,

---

Brett Phinney
Office 942-131-2075
Fax    942-131-2047
<email obscured>

Hi Kalina,
for many years I worked with the columns Reprosil from the small but reliable
German company "Dr Maisch", just look in the internet. They deliver fast and
have a wide variety of pore size, particle size and more.
Manfred

Am 11.12.18, 19:53 schrieb "ABRF Discussion Forum im Auftrag von Kalina
Dimova" <email obscured> im Auftrag von <email obscured>>:

    Hi,
    I am looking for recommendations on commercially available columns for
standard bottom up proteomics type samples (inlcuding TMT-labeling). We have a
nLC1000 coupled to a Q Exactive HF-X and have been using Thermo's Acclaim
columns because of the Viper fittings conveinence, but it is taking more than a
month to get a new column we ordered at the beginning of November
(made-to-order?!?) and we can't afford such long downtime..No expertise present
for in-house packing, so we are dependent on what is out there commercially.
    Thank you,
    Kalina
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Hi,
I am looking for recommendations on commercially available columns for standard
bottom up proteomics type samples (inlcuding TMT-labeling). We have a nLC1000
coupled to a Q Exactive HF-X and have been using Thermo's Acclaim columns
because of the Viper fittings conveinence, but it is taking more than a month
to get a new column we ordered at the beginning of November (made-to-order?!?)
and we can't afford such long downtime..No expertise present for in-house
packing, so we are dependent on what is out there commercially.
Thank you,
Kalina