In addition to software installation, you also need to perform autosampler calibration, wavelength correction. You need standard array to do wavelength.
Posts in ABRF Discussion Forum
Hi all, We have just one ABI 3130xl with a corrupt harddrive, and we don't have the data collection software. Does anyone have it somewhere where we could download it to get moving again? Our lab has stopped now for days, and Thermofisher said we need the CD to do a reinstall, but we can't find it (seems we never got it with the 2nd hand 3130 we bought). Especially if someone in Melbourne Australia has the CD we could borrow to make a copy?????? Vernon Muir DNA SOLUTIONS 03 9800 1550
Hi all, We have just one ABI 3130xl with a corrupt harddrive, and we don't have the data collection software. Does anyone have it somewhere where we could download it to get moving again? Our lab has stopped now for days, and Thermofisher said we need the CD to do a reinstall, but we can't find it (seems we never got it with the 2nd hand 3130 we bought). Especially if someone in Melbourne Australia has the CD we could borrow to make a copy?????? Vernon Muir DNA SOLUTIONS 03 9800 1550
fyi - open mass spec scientist position in Newark, CA. Contact Mary Heeney <email obscured>, 650-521-2884) if interested. www.intabio.com<http://www.intabio.com> Best, Allis Lab move news: https://mass-spec.stanford.edu/sums-move-bbb
____________________________________________________________ Allis Chien, Ph.D. Director, Stanford University Mass Spectrometry Vincent Coates Foundation Mass Spectrometry Laboratory Stanford Cancer Institute Proteomics Shared Resource 333 Campus Drive Seely G. Mudd Building, room 167A, MC 5080 Stanford, CA 94305-4401 Ph: 650.723.0710<tel:650.723.0710> Em: <email obscured><email obscured>> http://mass-spec.stanford.edu ____________________________________________________________
Hello all, I would like to know what your favorite software platform is for doing quality control of the sequence of cloned cDNA. The purpose of course is primarily to show that the cDNA you are using in your experiments is the correct sequence. But also should collaborators/journals/funding agencies require the proof that the clone is authentically what you say it is, you can provide them with a file or set of files as evidence. I've started looking around at software and see that DNA Baser sequence assembly software appears to have a nice interface which has the electropherogram above the text alignment in the same window. This would allow for efficient resolution of conflicts between different sequence runs. You can save the data for input later into the same program but I haven't seen how you would save data to provide to another who may not have this software, or to provide to a journal for e.g. supplementary data etc. Thanks in advance for any input.
Brian
All inputs here are very helpful. Thank you so much for the suggestions. Susie On Sun, Apr 21, 2019, 10:16 AM Hampton, Brian <email obscured>> wrote:
> Hello Susie, > > I've moved my lab several times, once between cities, twice between > buildings on campus and numerous times between labs within a building. In > my experience, the instrument vendors don't actually move the instruments, > maybe some do. The service people I had basically did a quick checkout to > verify working order, then shut it down, disconnect, and do the reverse > when the instrument arrives at the new location. I was responsible for the > moving. If something breaks during the move then its possible the mover's > liability insurance will cover it. That has never happened to me. I've > had movers transport triple quads, ion traps (not orbitrap) HPLCs Biacore > etc. No problem. Once, we purchased an Agilent MALDI-TOF back in the late > 1990s. It was fairly small and on wheels. It arrived on a truck wrapped > simply in plastic. The service engineer said they had less damage when the > delivery people could see the instrument through the plastic wrap versus > when it was crated and they could slam the crate around. > > Moving within a building with their smooth floors is easy if you have the > muscle power, and good carts, or a lift cart that can be positioned at the > bench height to slide heavy equipment off the bench to the cart at the same > height. I've done this by myself to move an LTQ. That instrument isn't > that big but wow is it heavy! A student and I moved an LTQ Orbitrap > "Classic" from one floor to another. It rolls fairly easy. The vacuum > pumps are moved separately and that was harder than moving the Orbitrap. > WRT the Orbitrap, the central electrode power supply is on the left side of > the instrument toward the bottom. It is held on with rubber isolation > mounts, which as rubber is prone to do, degrade with time and so that power > supply can drop down and then sit on the cables that connect through the > bottom of the power supply. This will happen just sitting still, but could > be accelerated during moving. So best to check these mounts, or try to add > some support to help it hang in place during the move. This should only be > a problem on older instruments such as the Classic etc that have been in > the field for some time. > > Moving between buildings, even if close, is best done via trucks because > they will provide a much smoother ride compared to rolling carts etc. down > the sidewalk. Anything prone to vibrational damage should go on a truck no > matter how small. > > If the path to the moving truck and back off at the new location is > smooth, then I would just push the orbitrap onto and off of the truck into > the new location. However, the transitions from hallways to loading docks > to trucks usually isn't smooth. So its best to have it palatized to allow > the movers to more "gently" traverse these transition points and use > lifting equipment to facilitate loading and off loading. Once on the truck > and secured, it will feel the same G-forces during the travel whether or > not is palatized. Possibly high frequency vibrations would be reduced if > palatized vs not. You could get a quote from Craters and Freighters if you > don't want to have Thermo do it. > > Finally, it probably wasn't your decision to move. So it would be logical > to conclude that those who found it necessary to move should have a budget > to pay for the move. You need the instruments to work at the new > location. So at least in the case of the Orbitrap, if Thermo guarantees > that it will work once moved - if they move it - then pay for them to do > just that. If your institution balks a paying for that level of service, > and it breaks in transit, then your institution should pay for repairs, if > the mover's liability insurance doesn't. For example, the central > electrode power supply for the Orbitrap "classic" costs about $22,000. > > Cheers, > > Brian > ________________________________ > From: ABRF Discussion Forum <email obscured>> on behalf of Bin Fang < > <email obscured>> > Sent: Saturday, April 20, 2019 7:39 PM > To: ABRF Discussion List > Subject: Re: [ABRF Discussion Forum] Mass spec moving service > > We did that 3 years ago. All bench top instruments should be easy to move. > Just take tons of pictures before you disconnect the components. Then you > can roll them on carts or benches with wheels. I'd be very scared to move > the old LTQ-orbitrap. That instrument is probably the only one I would ask > Thermo to move. > > > > > > On Sat, Apr 20, 2019 at 6:58 PM Mawuenyega, Kwasi <email obscured>> > wrote: > > > Hi Susie, > > > > My lab recently moved to a different building and I was in a similar > > situation. The solution I found (with a significant risk) was to hire a > > moving company to help with transportation. Professional movers are > insured > > to cover damages up to a certain cost and they can tell you if your > > expensive instruments can be covered under their insurance. If there is > > anyone in your lab that knows how to take the instruments apart, that > will > > save you a bunch of money. If the pumps and accessories are taken off, > the > > MS itself can be rolled down the hall on carts or transported gently > with a > > truck. There's no need to open the MS itself and tilting during > > transportation should be avoided at all cost. One Orbitrap Lumos, Fusion > > and triple quads were move and reassembled at the new location without a > > glitch. If you do not have anyone in-house to disassemble the > instruments, > > then you will have to go with the vendors to be safe. > > > > I hope this helps. > > > > Kwasi > > > > > > -----Original Message----- > > From: ABRF Discussion Forum <email obscured>> On Behalf Of Susie > > Sent: Thursday, April 18, 2019 10:14 AM > > To: ABRF Discussion List <email obscured>> > > Subject: [ABRF Discussion Forum] Mass spec moving service > > > > Hi all: > > We are moving our orbit trap, LTQ, and GCMS from one building to a new > one. > > The quotes we got from a vendor is very high. Any comments/thoughts/ideas > > for how to move? > > We moved the LTQ from one room to the other room by ourselves before. But > > now with a new building and an orbitrap, we kind of hope to get a better > > handle. > > Appreciate your inputs on this! > > Susie > > > > ―― > > View topic http://list.abrf.org/r/topic/436Q6zl7fFpEPORjoTjkwE > > Leave group <email obscured>?Subject=Unsubscribe > > > > > > ________________________________ > > The materials in this message are private and may contain Protected > > Healthcare Information or other information of a sensitive nature. If you > > are not the intended recipient, be advised that any unauthorized use, > > disclosure, copying or the taking of any action in reliance on the > contents > > of this information is strictly prohibited. If you have received this > email > > in error, please immediately notify the sender via telephone or return > mail. > > > > ―― > > View topic http://list.abrf.org/r/topic/biPdZIJ1TaoxeLqZuP6TG > > Leave group <email obscured>?Subject=Unsubscribe > > > > > > ―― > View topic http://list.abrf.org/r/topic/tTVlEhB5l4HtJhBpWzi31 > Leave group <email obscured>?Subject=Unsubscribe > > > ―― > View topic http://list.abrf.org/r/topic/1RIw8RXH6ztAmrH3xx2bAW > Leave group <email obscured>?Subject=Unsubscribe > >
Hi Everyone, I'm wondering if anyone can recommend any articles that document ionization efficiency of peptides with and without adducts, or any empirical studies that look at multiple parameters that affect ionization efficiency of peptides using ESI?
Best, Adam
matthias.trost
Senior Experimental Scientific Officer: Proteomics Core Manager (Newcastle University, UK)
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We are seeking a Proteomics Core Manager (successor of Achim Treumann). apply here: https://bit.ly/2vjVxOG Salary: £40,792 - £48,676 Closing date: 18 May 2019 Newcastle University Protein and Proteome Analysis (NUPPA) Core Facility is looking to recruit a Senior Experimental Scientific Officer to oversee and manage the high-end core technical facilities providing a state-of-the-art Mass Spectrometry, Proteomics and Protein Production Facility for researchers throughout the Faculty of Medical Sciences. You will provide the driving force to develop and maintain a world leading capability in this key set of technologies for the Faculty. You will lead a successful and experienced team of four core technologists offering Proteomics, Protein Production and Protein Biophysics services to all researchers within the University and also commercial partners. The post is required to manage, develop and promote these services within the University and to national and international customers, both academic and industrial. Possessing a relevant first degree or equivalent qualifications or experience, and ideally with a higher degree, you will be an experienced Proteomics professional with a biological background and experience in working within an academic research environment and/or core technology facility. Your technical skills should include extensive experience in LC-MS, MALDI-TOF, chromatographic and electrophoretic techniques, and up-to-date data analyses workflows. You should have experience of managerial responsibility for the scientific, business and financial planning of a group and an understanding of cost recovery regulations applied to a higher education setting. Informal enquiries in the first instance should be made to Matthias Trost <email obscured>) or Rebecca Kenyon <email obscured>) http://www.ncl.ac.uk/NUPPA/ Newcastle University is committed to being a fully inclusive Global University which actively recruits, supports and retains staff from all sectors of society. We value diversity as well as celebrate, support and thrive on the contributions of all our employees and the community they represent. We are proud to be an equal opportunities employer and encourage applications from everybody, regardless of race, sex, ethnicity, religion, nationality, sexual orientation, age, disability, gender identity, marital status/civil partnership, pregnancy and maternity, as well as being open to flexible working practices. click here for further details
Dear Manfred At least two more. AAA: Durrum D500 Protein sequencing: LKB solid phase sequencer Regards, Peter On 20/04/2019 11:30, Manfred@newtoncon wrote: > Hi, > > I am going to write a paper about the technical developments in Proteomics and before, as protein analysis. > > Working in protein analysis for many years, as many of you, we went through numerous technical and method developments – which disappeared quite fast and never came back, like peptide ladder sequencing. And we spenttime to follow such developments and found out, they do not help at all. Thinking back – I feel like a Proteomics tomb rider ☺ > > For this I am looking for information about: > > Protein sequencers (ABI, Beckman, Millipore, Knauer, Shimadzu and – if available – more) and C-terminal sequencers > > Amino acid analysers (ABI, HP, Beckman, LKB and who else) > > Preparative gel electrophoresis (ABI, Biorad, LKB……) > > Developments as the ABI plotter, the converted autosampler to a fraction collector), static nano spray, and what else comes into mind > > Analytical developments: MudPIT, in-source decay, peptide-ladder sequencing, PVDF plotting and sequencing…. > > I will also include mass spectrometers, like the first MALDIs, PDMS (I used one), early TSQs (had an API III, API 100), HPLC/FPLC equipment (still see some LKBs around), electrophoresis (still active but also some older developments), > > And what else comes in your mind, please throw it on me and please use mypersonal email: <email obscured> > > I am also looking for contributors – as co-authors, talked to some people and they like the idea, also to prevent the newbies to make the same errors we went through
> > Many thanks > > Manfred > > > > > ―― > View topic http://list.abrf.org/r/topic/5mQTpwQ3pNsTt8hr6V1GtA > Leave group <email obscured>?Subject=Unsubscribe >
Hi Manfred, Keith is right, listing just the keywords will generate as many characters as what is required to write a paper... Looking forward to dig into old transparencies, positive slides, hand-written paper, needle printer, all this to remember the beginning of the proteomics era... A few keywords: Definition of the word proteome in 1994 in Siena (M Wilkins) Definition of the word proteomics in 1996 in Siena (M Wilkins / R Appel) and published by P James in 1997 AA analysis and AACompIdent PMF, AMT LC-MALDI using a prototype lc spotter on a maldi plate The Molecular scanner The MALDI imaging development ICAT, TMT, ITRAC, SILAC, ... COFRADIC AQUA, SISCAPA Protein chips 2D-PAGE, 2D-DIGE SELDI And all the software and databases developed for this (see the Expasy.ch old-tools page for a start) (ExPASy was the first website dedicated to life sciences, and still exists since 1993)
Enjoy! Pierre-Alain -----Message d'origine----- De : ABRF Discussion Forum <email obscured>> De la part de Ashman, Keith Envoyé : dimanche 21 avril 2019 01:58 À : <email obscured> Objet : Re: [ABRF Discussion Forum] History of Proteomics and before Hi Manfred Sounds more like a book. Solid phase sequencing, peptide synthesis. 2d gels and in gel digest and the trypsin and other digest sin all their flavours come to mind Gruß Keith Sent from my iPhone > On 21 Apr 2019, at 3:01 am, Manfred@newtoncon <email obscured>> wrote: > > Hi, > > I am going to write a paper about the technical developments in Proteomics and before, as protein analysis. > > Working in protein analysis for many years, as many of you, we went > through numerous technical and method developments – which disappeared > quite fast and never came back, like peptide ladder sequencing. And we > spent time to follow such developments and found out, they do not help > at all. Thinking back – I feel like a Proteomics tomb rider ☺ > > For this I am looking for information about: > > Protein sequencers (ABI, Beckman, Millipore, Knauer, Shimadzu and – if > available – more) and C-terminal sequencers > > Amino acid analysers (ABI, HP, Beckman, LKB and who else) > > Preparative gel electrophoresis (ABI, Biorad, LKB……) > > Developments as the ABI plotter, the converted autosampler to a > fraction collector), static nano spray, and what else comes into mind > > Analytical developments: MudPIT, in-source decay, peptide-ladder sequencing, PVDF plotting and sequencing…. > > I will also include mass spectrometers, like the first MALDIs, PDMS (I > used one), early TSQs (had an API III, API 100), HPLC/FPLC equipment > (still see some LKBs around), electrophoresis (still active but also > some older developments), > > And what else comes in your mind, please throw it on me and please use > my personal email: <email obscured> > > I am also looking for contributors – as co-authors, talked to some > people and they like the idea, also to prevent the newbies to make the > same errors we went through > > Many thanks > > Manfred > > > > > ―― > View topic > https://urldefense.proofpoint.com/v2/url?u=http-3A__list.abrf.org_r_to > pic_5mQTpwQ3pNsTt8hr6V1GtA&d=DwIFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4X > JRvR6xMRXtA&r=Sj7Qby4YdMTJxPRgeiwA5rdssX98XecxgG3Wu_SFYGk&m=I1vyGhMvHg > FpIUNihraRhWiy-XUmRTaLCPgTg3catAs&s=wlBt6mT4vGewg7nvNs26_YgbDAqBvaKGEM > g-ftUlMjI&e= Leave group <email obscured>?Subject=Unsubscribe > Please be advised that this email may contain confidential information. If you are not the intended recipient, please notify us by email by replying to the sender and delete this message. The sender disclaims that the content of this email constitutes an offer to enter into, or the acceptance of, any agreement; provided that the foregoing does not invalidate the binding effect of any digital or other electronic reproduction of a manual signature that is included in any attachment. ―― View topic http://list.abrf.org/r/topic/7lPh8K3o27dFSRkUbCMSpe Leave group <email obscured>?Subject=Unsubscribe
I agree with Keith, Manfred, this sounds more like a book And it would be a valuable resource! Deb McMillen
> Hi, > > I am going to write a paper about the technical developments in Proteomics > and before, as protein analysis. > > Working in protein analysis for many years, as many of you, we went > through numerous technical and method developments â which disappeared > quite fast and never came back, like peptide ladder sequencing. And we > spent time to follow such developments and found out, they do not help at > all. Thinking back â I feel like a Proteomics tomb rider ⺠> > For this I am looking for information about: > > Protein sequencers (ABI, Beckman, Millipore, Knauer, Shimadzu and â if > available â more) and C-terminal sequencers > > Amino acid analysers (ABI, HP, Beckman, LKB and who else) > > Preparative gel electrophoresis (ABI, Biorad, LKBâ¦â¦) > > Developments as the ABI plotter, the converted autosampler to a fraction > collector), static nano spray, and what else comes into mind > > Analytical developments: MudPIT, in-source decay, peptide-ladder > sequencing, PVDF plotting and sequencingâ¦. > > I will also include mass spectrometers, like the first MALDIs, PDMS (I > used one), early TSQs (had an API III, API 100), HPLC/FPLC equipment > (still see some LKBs around), electrophoresis (still active but also some > older developments), > > And what else comes in your mind, please throw it on me and please use my > personal email: <email obscured> > > I am also looking for contributors â as co-authors, talked to some > people and they like the idea, also to prevent the newbies to make the > same errors we went through > > Many thanks > > Manfred > > > > > ââ > View topic http://list.abrf.org/r/topic/5mQTpwQ3pNsTt8hr6V1GtA > Leave group <email obscured>?Subject=Unsubscribe > > >
Hello Susie, I've moved my lab several times, once between cities, twice between buildings on campus and numerous times between labs within a building. In my experience, the instrument vendors don't actually move the instruments, maybe some do. The service people I had basically did a quick checkout to verify working order, then shut it down, disconnect, and do the reverse when the instrument arrives at the new location. I was responsible for the moving. If something breaks during the move then its possible the mover's liability insurance will cover it. That has never happened to me. I've had movers transport triple quads, ion traps (not orbitrap) HPLCs Biacore etc. No problem. Once, we purchased an Agilent MALDI-TOF back in the late 1990s. It was fairly small and on wheels. It arrived on a truck wrapped simply in plastic. The service engineer said they had less damage when the delivery people could see the instrument through the plastic wrap versus when it was crated and they could slam the crate around. Moving within a building with their smooth floors is easy if you have the muscle power, and good carts, or a lift cart that can be positioned at the bench height to slide heavy equipment off the bench to the cart at the same height. I've done this by myself to move an LTQ. That instrument isn't that big but wow is it heavy! A student and I moved an LTQ Orbitrap "Classic" from one floor to another. It rolls fairly easy. The vacuum pumps are moved separately and that was harder than moving the Orbitrap. WRT the Orbitrap, the central electrode power supply is on the left side of the instrument toward the bottom. It is held on with rubber isolation mounts, which as rubber is prone to do, degrade with time and so that power supply can drop down and then sit on the cables that connect through the bottom of the power supply. This will happen just sitting still, but could be accelerated during moving. So best to check these mounts, or try to add some support to help it hang in place during the move. This should only be a problem on older instruments such as the Classic etc that have been in the field for some time. Moving between buildings, even if close, is best done via trucks because they will provide a much smoother ride compared to rolling carts etc. down the sidewalk. Anything prone to vibrational damage should go on a truck no matter how small. If the path to the moving truck and back off at the new location is smooth, then I would just push the orbitrap onto and off of the truck into the new location. However, the transitions from hallways to loading docks to trucks usually isn't smooth. So its best to have it palatized to allow the movers to more "gently" traverse these transition points and use lifting equipment to facilitate loading and off loading. Once on the truck and secured, it will feel the same G-forces during the travel whether or not is palatized. Possibly high frequency vibrations would be reduced if palatized vs not. You could get a quote from Craters and Freighters if you don't want to have Thermo do it. Finally, it probably wasn't your decision to move. So it would be logical to conclude that those who found it necessary to move should have a budget to pay for the move. You need the instruments to work at the new location. So at least in the case of the Orbitrap, if Thermo guarantees that it will work once moved - if they move it - then pay for them to do just that. If your institution balks a paying for that level of service, and it breaks in transit, then your institution should pay for repairs, if the mover's liability insurance doesn't. For example, the central electrode power supply for the Orbitrap "classic" costs about $22,000.
Cheers, Brian ________________________________ From: ABRF Discussion Forum <email obscured>> on behalf of Bin Fang <email obscured>> Sent: Saturday, April 20, 2019 7:39 PM To: ABRF Discussion List Subject: Re: [ABRF Discussion Forum] Mass spec moving service We did that 3 years ago. All bench top instruments should be easy to move. Just take tons of pictures before you disconnect the components. Then you can roll them on carts or benches with wheels. I'd be very scared to move the old LTQ-orbitrap. That instrument is probably the only one I would ask Thermo to move. On Sat, Apr 20, 2019 at 6:58 PM Mawuenyega, Kwasi <email obscured>> wrote: > Hi Susie, > > My lab recently moved to a different building and I was in a similar > situation. The solution I found (with a significant risk) was to hire a > moving company to help with transportation. Professional movers are insured > to cover damages up to a certain cost and they can tell you if your > expensive instruments can be covered under their insurance. If there is > anyone in your lab that knows how to take the instruments apart, that will > save you a bunch of money. If the pumps and accessories are taken off, the > MS itself can be rolled down the hall on carts or transported gently with a > truck. There's no need to open the MS itself and tilting during > transportation should be avoided at all cost. One Orbitrap Lumos, Fusion > and triple quads were move and reassembled at the new location without a > glitch. If you do not have anyone in-house to disassemble the instruments, > then you will have to go with the vendors to be safe. > > I hope this helps. > > Kwasi > > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>> On Behalf Of Susie > Sent: Thursday, April 18, 2019 10:14 AM > To: ABRF Discussion List <email obscured>> > Subject: [ABRF Discussion Forum] Mass spec moving service > > Hi all: > We are moving our orbit trap, LTQ, and GCMS from one building to a new one. > The quotes we got from a vendor is very high. Any comments/thoughts/ideas > for how to move? > We moved the LTQ from one room to the other room by ourselves before. But > now with a new building and an orbitrap, we kind of hope to get a better > handle. > Appreciate your inputs on this! > Susie > > ―― > View topic http://list.abrf.org/r/topic/436Q6zl7fFpEPORjoTjkwE > Leave group <email obscured>?Subject=Unsubscribe > > > ________________________________ > The materials in this message are private and may contain Protected > Healthcare Information or other information of a sensitive nature. If you > are not the intended recipient, be advised that any unauthorized use, > disclosure, copying or the taking of any action in reliance on the contents > of this information is strictly prohibited. If you have received this email > in error, please immediately notify the sender via telephone or return mail. > > ―― > View topic http://list.abrf.org/r/topic/biPdZIJ1TaoxeLqZuP6TG > Leave group <email obscured>?Subject=Unsubscribe > > ―― View topic http://list.abrf.org/r/topic/tTVlEhB5l4HtJhBpWzi31 Leave group <email obscured>?Subject=Unsubscribe
Hi Manfred Sounds more like a book. Solid phase sequencing, peptide synthesis. 2d gels and in gel digest and the trypsin and other digest sin all their flavours come to mind Gruß Keith Sent from my iPhone > On 21 Apr 2019, at 3:01 am, Manfred@newtoncon <email obscured>> wrote: > > Hi, > > I am going to write a paper about the technical developments in Proteomics and before, as protein analysis. > > Working in protein analysis for many years, as many of you, we went through numerous technical and method developments – which disappeared quite fast and never came back, like peptide ladder sequencing. And we spent time to follow such developments and found out, they do not help at all. Thinking back – I feel like a Proteomics tomb rider ☺ > > For this I am looking for information about: > > Protein sequencers (ABI, Beckman, Millipore, Knauer, Shimadzu and – if available – more) and C-terminal sequencers > > Amino acid analysers (ABI, HP, Beckman, LKB and who else) > > Preparative gel electrophoresis (ABI, Biorad, LKB……) > > Developments as the ABI plotter, the converted autosampler to a fraction collector), static nano spray, and what else comes into mind > > Analytical developments: MudPIT, in-source decay, peptide-ladder sequencing, PVDF plotting and sequencing…. > > I will also include mass spectrometers, like the first MALDIs, PDMS (I used one), early TSQs (had an API III, API 100), HPLC/FPLC equipment (still see some LKBs around), electrophoresis (still active but also some older developments), > > And what else comes in your mind, please throw it on me and please use my personal email: <email obscured> > > I am also looking for contributors – as co-authors, talked to some people and they like the idea, also to prevent the newbies to make the same errors we went through > > Many thanks > > Manfred > > > > > ―― > View topic https://urldefense.proofpoint.com/v2/url?u=http-3A__list.abrf.org_r_topic_5mQTpwQ3pNsTt8hr6V1GtA&d=DwIFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=Sj7Qby4YdMTJxPRgeiwA5rdssX98XecxgG3Wu_SFYGk&m=I1vyGhMvHgFpIUNihraRhWiy-XUmRTaLCPgTg3catAs&s=wlBt6mT4vGewg7nvNs26_YgbDAqBvaKGEMg-ftUlMjI&e= > Leave group <email obscured>?Subject=Unsubscribe > Please be advised that this email may contain confidential information. If you are not the intended recipient, please notify us by email by replying to the sender and delete this message. The sender disclaims that the content of this email constitutes an offer to enter into, or the acceptance of, any agreement; provided that the foregoing does not invalidate the binding effect of any digital or other electronic reproduction of a manual signature that is included in any attachment.
We did that 3 years ago. All bench top instruments should be easy to move. Just take tons of pictures before you disconnect the components. Then you can roll them on carts or benches with wheels. I'd be very scared to move the old LTQ-orbitrap. That instrument is probably the only one I would ask Thermo to move. On Sat, Apr 20, 2019 at 6:58 PM Mawuenyega, Kwasi <email obscured>> wrote:
> Hi Susie, > > My lab recently moved to a different building and I was in a similar > situation. The solution I found (with a significant risk) was to hire a > moving company to help with transportation. Professional movers are insured > to cover damages up to a certain cost and they can tell you if your > expensive instruments can be covered under their insurance. If there is > anyone in your lab that knows how to take the instruments apart, that will > save you a bunch of money. If the pumps and accessories are taken off, the > MS itself can be rolled down the hall on carts or transported gently with a > truck. There's no need to open the MS itself and tilting during > transportation should be avoided at all cost. One Orbitrap Lumos, Fusion > and triple quads were move and reassembled at the new location without a > glitch. If you do not have anyone in-house to disassemble the instruments, > then you will have to go with the vendors to be safe. > > I hope this helps. > > Kwasi > > > -----Original Message----- > From: ABRF Discussion Forum <email obscured>> On Behalf Of Susie > Sent: Thursday, April 18, 2019 10:14 AM > To: ABRF Discussion List <email obscured>> > Subject: [ABRF Discussion Forum] Mass spec moving service > > Hi all: > We are moving our orbit trap, LTQ, and GCMS from one building to a new one. > The quotes we got from a vendor is very high. Any comments/thoughts/ideas > for how to move? > We moved the LTQ from one room to the other room by ourselves before. But > now with a new building and an orbitrap, we kind of hope to get a better > handle. > Appreciate your inputs on this! > Susie > > ―― > View topic http://list.abrf.org/r/topic/436Q6zl7fFpEPORjoTjkwE > Leave group <email obscured>?Subject=Unsubscribe > > > ________________________________ > The materials in this message are private and may contain Protected > Healthcare Information or other information of a sensitive nature. If you > are not the intended recipient, be advised that any unauthorized use, > disclosure, copying or the taking of any action in reliance on the contents > of this information is strictly prohibited. If you have received this email > in error, please immediately notify the sender via telephone or return mail. > > ―― > View topic http://list.abrf.org/r/topic/biPdZIJ1TaoxeLqZuP6TG > Leave group <email obscured>?Subject=Unsubscribe > >
Hi Susie, My lab recently moved to a different building and I was in a similar situation. The solution I found (with a significant risk) was to hire a moving company to help with transportation. Professional movers are insured to cover damages up to a certain cost and they can tell you if your expensive instruments can be covered under their insurance. If there is anyone in your lab that knows how to take the instruments apart, that will save you a bunch of money. If the pumps and accessories are taken off, the MS itself can be rolled down the hall on carts or transported gently with a truck. There's no need to open the MS itself and tilting during transportation should be avoided at all cost. One Orbitrap Lumos, Fusion and triple quads were move and reassembled at the new location without a glitch. If you do not have anyone in-house to disassemble the instruments, then you will have to go with the vendors to be safe. I hope this helps.
Kwasi -----Original Message----- From: ABRF Discussion Forum <email obscured>> On Behalf Of Susie Sent: Thursday, April 18, 2019 10:14 AM To: ABRF Discussion List <email obscured>> Subject: [ABRF Discussion Forum] Mass spec moving service Hi all: We are moving our orbit trap, LTQ, and GCMS from one building to a new one. The quotes we got from a vendor is very high. Any comments/thoughts/ideas for how to move? We moved the LTQ from one room to the other room by ourselves before. But now with a new building and an orbitrap, we kind of hope to get a better handle. Appreciate your inputs on this! Susie ―― View topic http://list.abrf.org/r/topic/436Q6zl7fFpEPORjoTjkwE Leave group <email obscured>?Subject=Unsubscribe ________________________________ The materials in this message are private and may contain Protected Healthcare Information or other information of a sensitive nature. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail.
Amino acid analysis: Waters PicoTag system (later AccQtag) http://www.waters.com/waters/library.htm?cid=511436&lid=1524232&locale=en_US <http://www.waters.com/waters/library.htm?cid=511436&lid=1524232&locale=en_US> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2547673/ <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2547673/> If Steve Cohen (of Waters) is still on this list he may have more information on this system. Ümit On 20Apr2019, at 13:49:38, Manfred@newtoncon <email obscured>> wrote: Sorry forgot the AGILENT sequencer and the protein workstation Manfred Am 20.04.19, 19:00 schrieb "ABRF Discussion Forum im Auftrag von Manfred@newtoncon" <email obscured> im Auftrag von <email obscured>>: Hi, I am going to write a paper about the technical developments in Proteomics and before, as protein analysis. Working in protein analysis for many years, as many of you, we went through numerous technical and method developments – which disappeared quite fast and never came back, like peptide ladder sequencing. And we spent time to follow such developments and found out, they do not help at all. Thinking back – I feel like a Proteomics tomb rider ☺ For this I am looking for information about: Protein sequencers (ABI, Beckman, Millipore, Knauer, Shimadzu and – if available – more) and C-terminal sequencers Amino acid analysers (ABI, HP, Beckman, LKB and who else) Preparative gel electrophoresis (ABI, Biorad, LKB……) Developments as the ABI plotter, the converted autosampler to a fraction collector), static nano spray, and what else comes into mind Analytical developments: MudPIT, in-source decay, peptide-ladder sequencing, PVDF plotting and sequencing…. I will also include mass spectrometers, like the first MALDIs, PDMS (I used one), early TSQs (had an API III, API 100), HPLC/FPLC equipment (still see some LKBs around), electrophoresis (still active but also some older developments), And what else comes in your mind, please throw it on me and please use my personal email: <email obscured> I am also looking for contributors – as co-authors, talked to some people and they like the idea, also to prevent the newbies to make the same errors we went through Many thanks Manfred ―― View topic http://list.abrf.org/r/topic/5mQTpwQ3pNsTt8hr6V1GtA Leave group <email obscured>?Subject=Unsubscribe
―― View topic http://list.abrf.org/r/topic/7lyUrYUiPvIHCSz7Qurssw Leave group <email obscured>?Subject=Unsubscribe
Sorry forgot the AGILENT sequencer and the protein workstation
Manfred
Am 20.04.19, 19:00 schrieb "ABRF Discussion Forum im Auftrag von
Manfred@newtoncon" <email obscured> im Auftrag von <email obscured>>:
Hi,
I am going to write a paper about the technical developments in Proteomics
and before, as protein analysis.
Working in protein analysis for many years, as many of you, we went through
numerous technical and method developments – which disappeared quite fast and
never came back, like peptide ladder sequencing. And we spent time to follow
such developments and found out, they do not help at all. Thinking back – I
feel like a Proteomics tomb rider ☺
For this I am looking for information about:
Protein sequencers (ABI, Beckman, Millipore, Knauer, Shimadzu and – if
available – more) and C-terminal sequencers
Amino acid analysers (ABI, HP, Beckman, LKB and who else)
Preparative gel electrophoresis (ABI, Biorad, LKB……)
Developments as the ABI plotter, the converted autosampler to a fraction
collector), static nano spray, and what else comes into mind
Analytical developments: MudPIT, in-source decay, peptide-ladder
sequencing, PVDF plotting and sequencing….
I will also include mass spectrometers, like the first MALDIs, PDMS (I used
one), early TSQs (had an API III, API 100), HPLC/FPLC equipment (still see some
LKBs around), electrophoresis (still active but also some older developments),
And what else comes in your mind, please throw it on me and please use my
personal email: <email obscured>
I am also looking for contributors – as co-authors, talked to some people
and they like the idea, also to prevent the newbies to make the same errors we
went through
Many thanks
Manfred
――
View topic http://list.abrf.org/r/topic/5mQTpwQ3pNsTt8hr6V1GtA
Leave group <email obscured>?Subject=Unsubscribe
Hi, I am going to write a paper about the technical developments in Proteomics and before, as protein analysis. Working in protein analysis for many years, as many of you, we went through numerous technical and method developments – which disappeared quite fast and never came back, like peptide ladder sequencing. And we spent time to follow such developments and found out, they do not help at all. Thinking back – I feel like a Proteomics tomb rider ☺ For this I am looking for information about: Protein sequencers (ABI, Beckman, Millipore, Knauer, Shimadzu and – if available – more) and C-terminal sequencers Amino acid analysers (ABI, HP, Beckman, LKB and who else) Preparative gel electrophoresis (ABI, Biorad, LKB……) Developments as the ABI plotter, the converted autosampler to a fraction collector), static nano spray, and what else comes into mind Analytical developments: MudPIT, in-source decay, peptide-ladder sequencing, PVDF plotting and sequencing…. I will also include mass spectrometers, like the first MALDIs, PDMS (I used one), early TSQs (had an API III, API 100), HPLC/FPLC equipment (still see some LKBs around), electrophoresis (still active but also some older developments), And what else comes in your mind, please throw it on me and please use my personal email: <email obscured> I am also looking for contributors – as co-authors, talked to some people and they like the idea, also to prevent the newbies to make the same errors we went through Many thanks
Manfred
Hi all: We are moving our orbit trap, LTQ, and GCMS from one building to a new one. The quotes we got from a vendor is very high. Any comments/thoughts/ideas for how to move? We moved the LTQ from one room to the other room by ourselves before. But now with a new building and an orbitrap, we kind of hope to get a better handle. Appreciate your inputs on this! Susie
I’ll get this to you by the end of the day Larry > On Apr 8, 2019, at 2:18 PM, Goldberg, Alisha R. <email obscured>> wrote: > > Thanks, Larry - Yes, I think I'm going to add my goofball skills to my resume - they come in handy at least as much as my education, which totally skipped the topic :) Thank you so much for the input. If you could make tracked changes and margin comments on this version we'll address as many as we can. > Alisha > > PS - Yes, the T is organized very differently - Glenda is working with Mark to tie it together with the others as much as possible. > > ________________________________ > From: Denner, Larry > Sent: Monday, April 08, 2019 8:22 AM > To: Goldberg, Alisha R. > Subject: > > Hi Alisha, > > You have done a remarkable job. I’m so impressed with your abilities to work with so many goofballs. Nonetheless, if you want substantive input from me on admin where I have a lot of minor thoughts, please send a word document where I can track changes. If there isn’t time for you or you just can’t stand any more crap from us (eh?), please consider the comments that follow with more that will undoubtedly emanate from further consideration. > > 1. I have suggestions of word choice in page 1. > > 2. On page 2, I encourage typical presentation with each aim being a new paragraph. This sets them apart from other elements, emphasizes the aims themselves rather than buried in text, and makes it easier for reviewers (probably most important). > > 3. Table A3 Network of PCOR Goal is missing P in Patient as the first letter. > 4. Table A4. Do you want to change ad interim nomenclature for Bhardwaj and Mouton > <GB Admin 4 7GB.docx>