Hello all, I just start to try digestion with SDC, I resuspended my protein pellet (Acetone precipitated) with 1% SDC in 50 mM HEPES buffer (pH 7.5) and when I add TCEP to 10 mM, my solution turned total milky. I have not clue what is going on. Should I use a different buffer instead of HEPES? Thank you! Zhuo Chen