Hello there
I have a 'problem' sample set derived from skin which was 'disrupted' in 5% SDS
followed by STRAP processing. I derived a good levels of tryptic peptides but
the samples are playing havoc with my ion funnel on a Lumos. I suspect lipids
are the issue. Question is what is my best chance to pull the peptides away
from the suspected lipid contaminants?
I would like to perform this on tryptic digests rather than the pre digest
extract as i need to recover this material (no more skin).
Any opinions on the merits of ion exchange versus HILIC or any other chemistry
that might help here?
Thanks for your help and have a great weekend
