Thanks for replying Scott.
The cycling parameters are
95 degrees for 3:30
Three Step: 95 for :15 then 50 for :15 and then 60 for 4:00
We have sequenced the gel purified PCR products and we see what I described
above. We have also tried sequencing the PCR product placed in a vector and
the QC drops off after the second repeat even though we are using our "RNAi"
mixture which contains betaine. We typically will use this if somebody is
expecting any type of repeat.
On the gel the band size appears to be correct.
