Post in Protein/peptide quantification for proteomics work flows: ABRF Discussion Forum: ABRF Discussion Forums

Hi Brian , we have had a lot of success with thermos fluorescent peptide assay . We still do traditional AAA so we checked it and it is pretty dead on . You need an expensive plate reader but it’s worth it. In fact we use this on all of our samples now and it’s improved everything. We know exactly how much we are loading on our columns now and how much we lose during digestion, peptide fractionation etc..l really recommend it Cheers Brett Sent from my iPad > On Sep 21, 2017, at 5:45 AM, Hampton, Brian <email obscured>> wrote: > > Hello all, > > I am considering the QuBit fluorometer for protein and peptide quantification to replace NanoDrop measurements at A280 for protein and A205 (Scopes) for peptides. The QuBit fluorometric system seems to be more sensitive ~250ng. I’m working with small pieces of tissues so I need sensitivity. The ability to also measure peptide concentration would be a real benefit too. I would appreciate any feed back from labs that use the QuBit and others who use other techniques to quantitate protein and peptides where sensitivity is an issue.