Hey Kim,
What mass spec are you using ?
Kind regards
Joel Steele
PhD candidate,
Casual Academic, B.MSc (Hons 1)
University of Technology Sydney
PO Box 123, Broadway, 2007
NSW, Australia
Ph:0434579993
https://www.linkedin.com/in/joel-r-steele
I acknowledge the traditional custodians of knowledge and land; the Gadigal
people of the Eora Nation, on whose land I live and work.
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________________________________
From: ABRF Discussion Forum <email obscured>> on behalf of James Farmar
<email obscured>>
Sent: Tuesday, 5 November 2019, 1:03 pm
To: ABRF Discussion List
Cc: Alex Yoon
Subject: Re: [ABRF Discussion Forum] Low level amino acid analyses
Kym,
Could try a filter on your vacuum release line. A filter pipet tip might
work to clean up your lab air.
Jim Farmar
On Wed, Oct 30, 2019 at 5:39 PM Kym Faull <email obscured>> wrote:
> We are attempting low level amino acid quantitation by LC/MS/MS-MRM. Our
> work is plagued by an unacceptably high background for certain amino acids
> (G, E, etc). We are looking for advice as to the most likely sources of
> contamination of amino acids. Samples in micro centrifuge tubes (including
> blanks) are dried in a speed vac, redissolved in an aqueous buffer to
> which is added a reagent. Following reaction the samples are transferred
> to HPLC injector vials and injected in to the LC/MS. All samples including
> the blanks have a variable and at times big signals for some amino acids.
> We seek advice as to the likley source(s) of contamination. Any and all
> advice will be appreciated
>
> Kym
>
> Kym Francis Faull, Ph.D.
> Director, Pasarow Mass Spectrometry Laboratory
> Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
> Professor Emeritus, Department of Psychiatry and Biobehavioral Sciences
> David Geffen School of Medicine at UCLA
> Telephone: (310) 206 7881 (office)
> Telephone: (310) 206 7886 (laboratory)
> Facsimile: (310) 206 2161
> Electronic mail: <email obscured> <email obscured>>
>
>
>
>
>
>
>
>
> ββ
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