Post in A sporadic but re-occurring problem with our Thermo QExactive plus unit

(bcc kym mario)

Are you writing to a network drive during acquisition?  if so, do the
data transfers after acquisition (you can trigger this with a post run
script)

if there is REALLY external network activity causing the issue,
perhaps try inserting a cheap home network router between your mass
spec and the wall plug.

I am really liking the other suggestions in this thread...

    * usb cables
    * acquisition PC is going bad  (perhaps try a memory check)
    * windows updates...

Rest of post




On Thu, Nov 18, 2021 at 5:48 AM Kristina Nelson <email obscured>> wrote:
>
> We had a somewhat similar problem with our Exploris 480.  The issue was
> narrowed down to the instrument PC, and the thinking was that there was a
> corrupted file somewhere on the PC itself.  Replacing the PC fixed the
> issue, but possibly it was just the install files that needed to be
> replaced.
> Good luck!
>
> Kristina Nelson
> PAE LC Team Lead
>
> On Thu, Nov 18, 2021 at 4:24 AM William Breuer <email obscured>>
> wrote:
>
> > Hi,
> > a few years ago we suffered from a similar chronic problem - our runs would
> > abort sporadically and we never knew when it would happen. We have a Dionex
> > Ultimate 3000 LC connected to a Q Exactive and the problem seemed to be
> > coming from the autosampler. Replacing the cable connecting the LC to the
> > computer did not help. In the end we replaced the whole autosampler (it
> > cost around $18,000). Since then the system has been behaving very well - 4
> > years now.....
> >
> > Bill Breuer
> > Proteomics/Mass spectrometry Unit,
> > Institute of Life Sciences,
> > Edmond J. Safra Campus - Givat Ram,
> > Hebrew University of Jerusalem,
> > Jerusalem, 9190401
> > Israel
> >
> > On Thu, Nov 18, 2021 at 4:28 AM Susie <email obscured>> wrote:
> >
> > > Do you have an automatic update function turning on for your mass spec
> > PC?
> > > Can you turn it off? If you Google the cited language，people are taking
> > > about proxy settings. You may want to adjust that, but take careful notes
> > > for what you change d, so you can always change back.
> > >
> > > Not sure this helps or not. Unless the lab policy has requirements for
> > LIMS
> > > and online data transfer, my approach for mass spectrometers is do not go
> > > to internet unless it is very necessary. Our IT folks don't like it, but
> > I
> > > am able to bypass the IT folks that my mass spec does not need internet.
> > > When I need internet, we use wireless jump drives.
> > > I am not an individual who believes "ware" updates for mass spec. Many
> > > years ago, I  lost my confidence after our GCMS service engineer
> > convinced
> > > me to update my firmware when he was doing our PM. I ended up with losing
> > > all my methods and had to rebuild all of them.
> > > Susie
> > >
> > >
> > >
> > > On Wed, Nov 17, 2021, 5:42 PM Kym Faull <email obscured>> wrote:
> > >
> > > > > Dear Colleagues:   We have a problem with our LC/MS QE instrument.
> > > Long
> > > > ago we had a problem with it in which every Thursday morning at the
> > same
> > > > time the instrument would quit because of some glitch with the internet
> > > > connection.  We concluded that a strange signal from the internet was
> > > > causing the instrument to quit.  We implemented a work-around in which
> > > the
> > > > internet connection was disabled for the time around when this was
> > > > happening, and the connection was re-established after the elapsed
> > time.
> > > > This work-around worked for a long time with occasional instances at
> > > other
> > > > times when the problem would sporadically  pop-up.  The situation has
> > > > recently changed.  The hiccup is now occurring frequently.  It has
> > > recently
> > > > occurred  10 times in 4 days.   The error messages that are reported
> > are
> > > > copied below.  Of course we have been run touch with Thermo people, but
> > > so
> > > > far the problem has not been solved.  Any and all advice about this
> > will
> > > be
> > > > greatly appreciated.
> > > >
> > > > Kym
> > > >
> > > >
> > > > Kym Francis Faull, Ph.D.
> > > > Director, Pasarow Mass Spectrometry Laboratory
> > > > Jane and Terry Semel Institute for Neuroscience and Human Behavior at
> > > UCLA
> > > > Professor Emeritus on Recall, Department of Psychiatry and
> > Biobehavioral
> > > > Sciences
> > > > David Geffen School of Medicine at UCLA
> > > > Telephone: (310) 206 7881 (office)
> > > > Telephone: (310) 206 7886 (laboratory)
> > > > Facsimile: (310) 206 2161
> > > > Electronic mail: <email obscured> <email obscured>>
> > > >
> > > >
> > > >
> > > >
> > > >
> > > >
> > > >
> > > >
> > > > > Begin forwarded message:
> > > > >
> > > > > From: Julian Whitelegge <email obscured>>
> > > > > Subject: Re: we need help
> > > > > Date: November 16, 2021 at 1:28:57 PM PST
> > > > > To: Kym Faull <email obscured>>
> > > > >
> > > > > Kym, Have you tried posting the error message on the ABRF site ? I
> > > can’t
> > > > believe we are the only ones with this problem... Julian
> > > > >
> > > > >> On Nov 16, 2021, at 1:26 PM, Kym Faull <email obscured>
> > <mailto:
> > > > <email obscured>>> wrote:
> > > > >>
> > > > >> Kent:  We have done everything you suggest and the problem still
> > > > occurs.  Sometimes it occurs shortly after setting a worklist running,
> > > and
> > > > last weekend it ran uninterrupted from Friday until sometime on Sunday
> > > when
> > > > it quit.  Attached below is the error message that appears when the
> > > > problems occurs.  We don’t know what t o do next,  Can you help us?
> > > Before
> > > > we request a visit from a service engineer I thought a ZOOM or WEbEX
> > > > “meeting” might be the next thing to try.
> > > > >>
> > > > >>
> > > > >> Kym
> > > > >>
> > > > >>
> > > > >>
> > > > >>> Begin forwarded message:
> > > > >>>
> > > > >>> From: whitaker cohn <email obscured> <email obscured>
> > >>
> > > > >>> Subject: Fw: QE Error Message
> > > > >>> Date: November 11, 2021 at 9:03:33 AM PST
> > > > >>> To: Kym Faull <email obscured> <email obscured>>>
> > > > >>>
> > > > >>> The QE turned off again last night. These were the error messages
> > at
> > > > the time of the event:
> > > > >>>
> > > > >>> -Info       Service 18:40:20.142-07:00 on 2021-11-04
> > Starting
> > > > acquisition
> > > > >>>
> > > > >>> -Warning    Service 19:49:55.603-07:00 on 2021-11-04        reading
> > > > data: A connection attempt failed because the connected party did not
> > > > properly respond after a period of time, or established connection
> > failed
> > > > because connected host has failed to respond
> > > > >>>
> > > > >>> -Warning    Service 19:49:57.069-07:00 on 2021-11-04        reading
> > > > data: A connection attempt failed because the connected party did not
> > > > properly respond after a period of time, or established connection
> > failed
> > > > because connected host has failed to respond
> > > > >>>
> > > > >>> -Warning    Service 19:49:57.132-07:00 on 2021-11-04        reading
> > > > data: A connection attempt failed because the connected party did not
> > > > properly respond after a period of time, or established connection
> > failed
> > > > because connected host has failed to respond
> > > > >>>
> > > > >>> -Error      Instrument      19:50:04.601-07:00 on 2021-11-04
> > > > [Inspect/Networking/Control handler] No network connection to data
> > system
> > > > (s)
> > > > >>>
> > > > >>> I think we need to focus on remedying "A connection attempt failed
> > > > because the connected party did not properly respond after a period of
> > > > time, or established connection failed because connected host has
> > failed
> > > to
> > > > respond". Not sure how to do this. Maybe Boston has some ideas.
> > > > >>>
> > > > >>> This also occurs when the instrument is not connected to the
> > > internet.
> > > > It would be great if these error messages could be shared among the
> > > Thermo
> > > > instrument technicians. Somebody has to have encountered this before...
> > > > >>>
> > > > >>> Sincerely,
> > > > >>> Whitaker
> > > > >>>
> > > > >>>
> > > > >>>
> > > > >>>
> > > > >>>
> > > > >>>
> > > > >>>
> > > > >>> Whitaker Cohn, B.S.
> > > > >>> Ph.D. Candidate
> > > > >>> Drug Discovery Lab
> > > > >>> Pasarow Mass Spectrometry Lab
> > > > >>> UCLA | Molecular, Cellular & Integrative Physiology Program
> > > > >>> (818) 268-5795
> > > > >>>
> > > > >>>
> > > > >>
> > > > >
> > > >
> > > >
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