What a great read this morning! Thank you Brian and Andy for sharing this paper. Although retired, I very much appreciate (occasionally) reading current techniques, especially those that build on and refine technique. Ammonium sulfate cuts of detergent solubilized beef heart cytochrome c oxidase preparations and lipid head group identification of the remaining tightly bound lipids was a large part of my first 10 years in science labs (we are talking the mid '70's to '80's here. The scale of material we worked on with ultracentrifugation rotors was so different than where we are today. It has been fantastic to see such progress! Thanks for the read with my morning coffee! Deb McMillen A long-time helpful contributor to this list, Andy Alpert, made me aware of this paper which is yet another very interesting approach to isolating membrane proteins. For my purposes (bottom up proteomics), the use of Triton X114 isn't a problem because removal is easily achieved after digestion using SDB-RPS or similar tips in HILIC/SCX mode as described in the EasyPhos Nature Methods paper. Thanks Andy for your input! "Top-Down Proteomics of Endogenous Membrane Proteins Enabled by Cloud Point Enrichment and Multidimensional Liquid Chromatography−Mass Spectrometry" [ https://dx.doi.org/10.1021/acs.analchem.0c02533 | https://dx.doi.org/10.1021/acs.analchem.0c02533 ] Best regards, Brian