Hi Kym,
When we did PicoTag amino acid analysis, we used Corning 5 x 50 mm Pyrex
culture tubes cat. number 9820. These glass tubes were pyrolized at ~~ 300
deg. C ( maybe higher, can't find the temp we used) to carbonize organics.
Samples were dried in these tubes and processed for AAA. By doing the
pyrolysis we at least knew any source of contamination was not coming from
those tubes.
Good luck,
Rest of post
Brian
________________________________
From: ABRF Discussion Forum <email obscured>> on behalf of Kym Faull
<email obscured>>
Sent: Wednesday, October 30, 2019 5:39 PM
To: <email obscured> <email obscured>>
Cc: Alex Yoon <email obscured>>
Subject: [ABRF Discussion Forum] Low level amino acid analyses
We are attempting low level amino acid quantitation by LC/MS/MS-MRM. Our work
is plagued by an unacceptably high background for certain amino acids (G, E,
etc). We are looking for advice as to the most likely sources of contamination
of amino acids. Samples in micro centrifuge tubes (including blanks) are
dried in a speed vac, redissolved in an aqueous buffer to which is added a
reagent. Following reaction the samples are transferred to HPLC injector vials
and injected in to the LC/MS. All samples including the blanks have a variable
and at times big signals for some amino acids. We seek advice as to the likley
source(s) of contamination. Any and all advice will be appreciated
Kym
Kym Francis Faull, Ph.D.
Director, Pasarow Mass Spectrometry Laboratory
Jane and Terry Semel Institute for Neuroscience and Human Behavior at UCLA
Professor Emeritus, Department of Psychiatry and Biobehavioral Sciences
David Geffen School of Medicine at UCLA
Telephone: (310) 206 7881 (office)
Telephone: (310) 206 7886 (laboratory)
Facsimile: (310) 206 2161
Electronic mail: <email obscured> <email obscured>>
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