Dear ARG Members, I have a question about using peptide pools conjugated to carrier protein for immunization. I have an investigator who wants to produce a monoclonal antibody targeting a specific histidine residue in/near the active site of a particular membrane protein to block enzymatic activity. The PI identified 3 peptides that were predicted to be immunogenic. Rather than pick one, I suggested using all three peptides. Also, I suggested that equimolar amounts of the three peptides could be conjugated to the same carrier molecule, since the peptides are identical except 3-4 amino acid differences. I spoke with a technical rep from Thermo Scientific, and this person assured me that this is a common practice. However, I have only been able to find one example of a somewhat similar approach in the literature (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015836/). Is this approach commonly used? Do you know of a publication that has used peptide pools to produce site directed antibodies? Thank you for your advice! Angel Edith “Angel” Sampson, Ph.D. Monoclonal Antibody Scientific Director UF ICBR 2033 Mowry Road, Gainesville, FL 32610 Biotech.ufl.edu | Tel: 352.273.8039| Fax: 352.273.8070