There should not be any non-volatile salts left. There may be some small amount of ammonium formate or trifluoroacetate formed during elution depending on which acid you used in the final wash prior to elution.
Brian ________________________________ From: ABRF Discussion Forum <email obscured>> on behalf of Noah D4 <email obscured>> Sent: Monday, December 14, 2020 10:33 PM To: <email obscured> <email obscured>> Subject: Re: [ABRF Discussion Forum] What are people's favorite phosphopeptide enrichment methods and protocols? CAUTION: This message originated from a non UMB, UMSOM, FPI, or UMMS email system. Whether the sender is known or not known, hover over any links before clicking and use caution opening attachments. Brian, Thanks again for all the info and discussion. After drying the NH4OH/MeCN eluate, is it sufficiently salt-free for mass spec analysis (I don't use a trap column)? Noah ―― View topic https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flist.abrf.org%2Fr%2Ftopic%2F3q3edFho0HTGIFVj6ln6lL&data=04%7C01%7Cbhampton%40som.umaryland.edu%7C257f5a8e0df244ecf9e508d8a0aa316b%7C717009a620de461a88940312a395cac9%7C0%7C0%7C637436000248526326%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=nREnGGCBTg9J5eil8JDG3SLdhTtKOCLvr3VNZJSWBE4%3D&reserved=0 Leave group <email obscured>?Subject=Unsubscribe