I would do a recovery and a leaching test because it will be dependent on the
specific lot and history of the container and the details of your prep (what
the EVs are in how you recover them). Polyallomer is closest to a material we
all use more frequently than polycarbonate though that has its problems -
additives in either could be an issue though it is true there are ways around
that.
I would also add that doing a comparison with the gel filtration methods (Izon
qEV....) is worthwhile, just in case it is better etc. My submitters seem to
favor this format now. These can be hard samples to prepare and especially
challenging when you are doing quantitative comparisons between two samples and
don't want to end up with false positives coming from small differences in prep
execution.
My 0.11 cents
D. Eric Anderson, Ph.D
Advanced Mass Spectrometry Core Facility, NIDDK
NIH
Building 8 Room B2A19, 9000 Rockville Pike, Bethesda MD, 20892 USA
(301)-496-7546
Rest of post
________________________________________
From: Yan Wang <email obscured>>
Sent: 23 April 2021 09:22
To: ABRF Discussion List
Subject: Re: [ABRF Discussion Forum] polymers and mass spectrometry
I don't know the tube material well, but if you do protein precipitation or
use S-Trap for digestion, polymer contamination at this stage may not
matter. My 1 cent.
Yan
On Fri, Apr 23, 2021 at 9:20 AM turck <email obscured>> wrote:
> We are planning to isolate exosomes by ultracentrifugation. We have tubes
> made from either polycarbonate or polyallomer. Since we want to use mass
> spectrometry, we are worried about polymer contamination. What type of tube
> material do you recommend? Polycarboante or polyallomer or something else
> altogether?
> ββ
> View topic http://list.abrf.org/r/topic/2VMr7ZT5lrqt9F6xgywyFf
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>
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