We are having a technical issue sequencing a repeated symmetric region. This PCR product is 332bp long and it includes three symmetric lacOs without gaps in between. The sequencing results suggest that when it finished the first lacO, it somehow "hopped" to the complement strand and sequenced back to the origin. We see this with both forward and reverse primer sequencing showing this "hopping" effect. Please see our attached figure for more information. We simply cannot figure out what is going on here. The data was verified by resequencing with a different facility. Please let us know if you have also experienced this before and have some solution to resolve issue! See attached simple drawing for example....