Hi Brian, one thing to watch out for is that the kit requires filters that
don't seem to be standard on a lot of plate readers so you may want to
check before you buy the kit
Cheers
Brett
On Thu, Sep 21, 2017 at 11:24 AM Hampton, Brian <email obscured>>
wrote:
Rest of post
> Hello Brett,
>
> I’ll give this assay kit a try! It turns out that the QuBit has a blue
> and red LED light source. So the Ex/Em wavelengths are limited and not
> compatible with the peptide assay kit you mention. I do have access to a
> fluorescent plate reader so I’m good to go.
>
> Thanks,
>
> Brian
>
> > On Sep 21, 2017, at 11:27 AM, Brett Phinney <email obscured>> wrote:
> >
> > Hi Brian , we have had a lot of success with thermos fluorescent peptide
> assay . We still do traditional AAA so we checked it and it is pretty dead
> on . You need an expensive plate reader but it’s worth it. In fact we use
> this on all of our samples now and it’s improved everything. We know
> exactly how much we are loading on our columns now and how much we lose
> during digestion, peptide fractionation etc..l really recommend it
> >
> > Cheers
> >
> > Brett
> >
> >
> >
> > Sent from my iPad
> >
> >> On Sep 21, 2017, at 5:45 AM, Hampton, Brian <email obscured>>
> wrote:
> >>
> >> Hello all,
> >>
> >> I am considering the QuBit fluorometer for protein and peptide
> quantification to replace NanoDrop measurements at A280 for protein and
> A205 (Scopes) for peptides. The QuBit fluorometric system seems to be more
> sensitive ~250ng. I’m working with small pieces of tissues so I need
> sensitivity. The ability to also measure peptide concentration would be a
> real benefit too. I would appreciate any feed back from labs that use the
> QuBit and others who use other techniques to quantitate protein and
> peptides where sensitivity is an issue.
> >>
> >> Thanks!
> >>
> >> Brian
> >> ――
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